TSLP promotes angiogenesis of human umbilical vein endothelial cells by strengthening the crosstalk between cervical cancer cells and eosinophils

Zhang, Bing; Wei, Chunyan; Chang, Kai‐Kai; Yu, Jiajun; Zhou, Wen‐Jie; Yang, Hui‐Li; Shao, Jun; Yu, Jinjin; Li, Ming‐Qing; Xie, Feng

doi:10.3892/ol.2017.7121

Cited by 21 publications

(25 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similarly, increased miR-205 was also previously observed in cervical squamous cell carcinoma cells . Consistently, Zhang et al (2017) revealed that miR-205 inhibited CC cell proliferation via olmesartan, which served as a therapeutic target for CC (Yue, Yun-Shan, & Feng-Xia, 2016). Meanwhile, a previous study demonstrated that poor expression of TSLC1 (also CADM1) was implicated in high risk HPV-mediated CC (Overmeer et al, 2008).…”

Section: Downregulation Of Mir-205 Suppresses Angiogenesis By Elevamentioning

confidence: 85%

RETRACTED: Downregulation of microRNA‐205 inhibits cell invasion and angiogenesis of cervical cancer through TSLC1‐mediated Akt signaling pathway

Zhang

Liu

Xie

2019

Journal Cellular Physiology

View full text Add to dashboard Cite

Cervical cancer (CC) is a common gynecological cancer and a leading cause of cancer‐related deaths in women globally. Therefore, this study explores the action of microRNA‐205 (miR‐205) in the invasion, migration, and angiogenesis of CC through binding to tumor suppressor lung cancer 1 (TSLC1). Initially, the microarray analysis was used to select the candidate gene and the regulatory microRNA. Then, the target relationship between miR‐205 and TSLC1 as well as the expression of miR‐205, TSLC1, and p‐Akt/total Akt in CC cells were determined. Afterwards, CC cell invasion and migration were detected after the treatment of miR‐205 mimics/inhibitors and short hairpin RNA against TSLC1. After coculture of cancer cells and vascular endothelial cells, cell proliferation, tube formation, and microvessel density (MVD) were detected to determine the roles of miR‐205 in angiogenesis. Finally, tumor growth in nude mice was measured in vivo. TSLC1 was determined as the candidate gene, which was found to be targeted and negatively regulated by miR‐205. Then, downregulated miR‐205 or forced TSLC1 expression inhibited invasion, migration, and angiogenesis in CC, corresponding to suppressed cell proliferation, tube formation, and expression of IL‐8, VEGF, and bFGF, as well as the inhibited activation of the Akt signaling pathway. Furthermore, downregulation of miR‐205 was found to exert an inhibitory role in tumor formation and MVD by elevating TSLC1 in CC in vivo. This study demonstrated that downregulated miR‐205 inhibited cell invasion, migration, and angiogenesis in CC by inactivating the Akt signaling pathway via TSLC1 upregulation.

show abstract

Section: Downregulation Of Mir-205 Suppresses Angiogenesis By Elevamentioning

confidence: 85%

RETRACTED: Downregulation of microRNA‐205 inhibits cell invasion and angiogenesis of cervical cancer through TSLC1‐mediated Akt signaling pathway

Zhang

Liu

Xie

2019

Journal Cellular Physiology

View full text Add to dashboard Cite

show abstract

“…Several studies reported a pro-tumor role for TSLP in cervical cancer (36)(37)(38). Tumor cells under hypoxia expressed TSLP, and TSLPR was expressed in both tumor cells and vascular endothelial cells.…”

Section: Cervical Cancermentioning

confidence: 99%

Thymic Stromal Lymphopoietin and Cancer: Th2-Dependent and -Independent Mechanisms

Protti

Monte

2020

Front. Immunol.

View full text Add to dashboard Cite

The thymic stromal lymphopoietin (TSLP) is an IL-7-like cytokine originally cloned from a murine thymic stromal cell line, and subsequently a human homolog was identified using database search methods. Human TSLP is mostly expressed in epithelial cells, among which are keratinocytes as well as stromal cells such as fibroblasts and immune cells. Human TSLP was first described to activate myeloid dendritic cells, which prime naïve T helper cells to produce high concentrations of Th2 cytokines, thus representing a key cytokine in triggering dendritic cells-mediated allergic Th2 inflammation. TSLP and/or its receptor has been shown to be expressed in several tumor types, where TSLP expression is associated with functional activities that can be associated or not with the induction of a Th2-prone tumor microenvironment, i.e., Th2-dependent and Th2-independent mechanisms. These mechanisms involve tissue-and immune cell target-dependent tumor-promoting or tumor-suppressive functions in different or even the same tumor type. Here we report and discuss the Th2-dependent and Th2-independent roles of TSLP in cancer and possible therapeutic targeting.

show abstract

“…Notably, this finding might explain the concomitant presence of circulating DCs polarized into a tolerogenic phenotype, and the expanded T reg pool found in PDCA patients [ 4 , 6 ]. Moreover, TSLP produced by cervical cancer cells may promote angiogenesis [ 24 , 25 ], and down-regulate miR-132 expression, favoring tumor development [ 26 ]. The involvement of TSLP in tumor growth is also supported by several murine experimental studies, in which tumor growth was inhibited in TSLP-deficient hosts [ 27 , 28 ].…”

Section: Discussionmentioning

confidence: 99%

Thymic stromal lymphopoietin in human pancreatic ductal adenocarcinoma: expression and prognostic significance

et al. 2018

View full text Add to dashboard Cite

Thymic stromal lymphopoietin (TSLP) has emerged as an important, but contradictory, player conditioning tumor growth. In certain contexts, by driving T helper (h) 2 responses via tumor-associated OX40 Ligand (OX40L)+ dendritic cells (DCs), TSLP may play a pro-tumorigenic role. The study elucidates the importance of TSPL in pancreatic ductal adenocarcinoma (PDAC), by analyzing: i) TSLP levels in PDAC cell-line supernatants and plasma from patients with locally-advanced/metastatic PDAC, pre- and post-treatment with different chemotherapeutic protocols, in comparison with healthy donors; ii) TSLP and OX40L expression in PDAC and normal pancreatic tissues, by immunohistochemistry; iii) OX40L expression on ex vivo-generated normal DCs in the presence of tumor-derived TSLP, by flow cytometry; iv) clinical relevance in terms of diagnostic and prognostic value and influence on treatment modality and response.Some PDAC cell lines, such as BxPC-3, expressed both TSLP mRNA and protein. Normal DCs, generated ex vivo in the presence of TSLP-rich-cell supernatants, displayed increased expression of OX40L, reduced by the addition of a neutralizing anti-TSLP polyclonal antibody. OX40L+ cells were detected in pancreatic tumor inflammatory infiltrates. Abnormally elevated TSLP levels were detected in situ in tumor cells and, systemically, in locally-advanced/metastatic PDAC patients. Of the chemotherapeutic protocols applied, gemcitabine plus oxaliplatin (GEMOX) significantly increased circulating TSLP levels. Elevated plasma TSLP concentration was associated with shorter overall survival and increased risk of poor outcome. Plasma TSLP measurement successfully discriminated PDAC patients from healthy controls.These data show that TSLP secreted by pancreatic cancer cells may directly impact PDAC biology and patient outcome.

show abstract

TSLP promotes angiogenesis of human umbilical vein endothelial cells by strengthening the crosstalk between cervical cancer cells and eosinophils

Cited by 21 publications

References 39 publications

RETRACTED: Downregulation of microRNA‐205 inhibits cell invasion and angiogenesis of cervical cancer through TSLC1‐mediated Akt signaling pathway

RETRACTED: Downregulation of microRNA‐205 inhibits cell invasion and angiogenesis of cervical cancer through TSLC1‐mediated Akt signaling pathway

Thymic Stromal Lymphopoietin and Cancer: Th2-Dependent and -Independent Mechanisms

Thymic stromal lymphopoietin in human pancreatic ductal adenocarcinoma: expression and prognostic significance

Contact Info

Product

Resources

About