TSH and TSH Receptor Antibody-Binding Sites in Fibroblasts of Pretibial Myxedema Are Related to the Extracellular Domain of Entire TSH Receptor

Chang, Tien–Chun; Wu, Shih-Lu; Hsiao, Yung-Lien; Kuo, Shu-Ting; Chien, Li-Fei; Kuo, Yea-Fhey; Chang, Chih‐Cheng; Chang, Tien‐Jye

doi:10.1006/clin.1994.1059

Cited by 43 publications

(23 citation statements)

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“…Liquid hybridization analysis and quantitation of TSHR mRNA Strongly positive protected bands at 298 bp and 217 bp, indicating the presence of intact (2.4 kb) and variant (1.3 kb) TSHR mRNA, respectively, were apparent in LHA gel lanes corresponding to normal thyroid, Graves (1) undigested probe: (2) digested probe (321 bp); (3)(4)(5)(6)(7)(8) standard curve; (9) thymus; (10) adrenal; (11) kidney; (12) testis; (13) normal thyroid; (14) Graves' thyroid; (15) molecular standards. Positive protected bands at 298 bp (corresponding to 2.4 kb intact TSHR) and 217 bp (corresponding to 1.3-kb variant form TSHR) are ap¬ parent in lanes containing RNA from Graves' thyroid, normal thyroid, kidney (faintly positive), adrenal, and thymus.…”

Section: Resultsmentioning

confidence: 99%

“…The negative Northern blotting re¬ sults in adrenal and kidney (despite the presence of bands corresponding to these tissues on LHA) are in keeping with the lesser sensitivity of Northern blotting as compared with LHA for detection of TSHR. (6) kidney; (7) thymus; (8) liver; (9) skeletal muscle; (10) testis; (11) brain; (12) normal thyroid; (13) Graves' thyroid. Positive protected bands at 316 bp, indicating the presence of equal quantities of GADPH RNA, are apparent in lanes 3-13.…”

Section: Northern Blot Analysismentioning

confidence: 99%

“…Because this receptor is considered a candidate antigen involved in the extrathyroidal manifestations of Graves' disease, its presence has been sought in human orbital and skin tis¬ sues (1). Both full-length TSHR mRNA and a 1.3-kb vari¬ ant TSHR transcript have been detected in normal and Graves' orbital connective tissues using reverse-transcriptase polymerase chain reaction (RT-PCR) (2)(3)(4)(5)(6)(7), and thy¬ rotropin (TSH)-like immunoreactivity has been reported in these tissues (8,9). In addition, two recent studies have demonstrated TSHR mRNA in normal thymus using Northern blotting or RT-PCR (10,11).…”

Section: Introductionmentioning

confidence: 99%

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Thyrotropin Receptor Expression in Adrenal, Kidney, and Thymus

Dutton

Joba

Spitzweg

et al. 1997

Thyroid

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Because the thyrotropin receptor (TSHR) has long been considered a thyroid-specific protein, its presence in extrathyroidal tissues has been controversial. In this study, we sought to detect and quantify this potentially low abundance mRNA in various extrathyroidal tissues using liquid hybridization analysis (LHA) and to detect protein with immunohistochemical studies. Strongly positive protected bands, indicating the presence of both intact (2.4 kb) and variant (1.3 kb) TSHr mRNA, were apparent in LHA gel lanes corresponding to normal thyroid, Graves' thyroid, and thymus. Less abundant protected bands of the same sizes were present in lanes corresponding to normal adrenal, and samples from normal kidney were faintly positive. The full-length transcript:variant transcript ratio was approximately 1:1 in all positive tissues. Immunohistochemical analysis of TSHR-like reactivity in paraffin-embedded thymus, adrenal, and kidney revealed specific staining in each of these tissues. No TSHR mRNA or TSHR-like immunoreactivity was detected in samples from several other normal human tissues. We conclude that measurable TSHR mRNA and protein expression is not restricted to the thyroid gland. Further study is warranted to determine whether these extrathyroidal receptors play a role in normal physiology or in disease.

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Section: Resultsmentioning

confidence: 99%

Section: Northern Blot Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Thyrotropin Receptor Expression in Adrenal, Kidney, and Thymus

Dutton

Joba

Spitzweg

et al. 1997

Thyroid

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“…Histology reveals an excessive dermal and subcutaneous deposition of glycosaminoglycans, the mechanism of which is not understood. There is, however, some in vitro evidence that anti-thyroid stimulating hormone receptor antibodies (anti-TSH receptor) may be directed against thyroid antigens on pretibial skin fibroblasts and adipocytes stimulating them to secrete large amounts of glycosaminoglycans [47,48].…”

Section: Pretibial Myxedemamentioning

confidence: 99%

Use of IGIV in the treatment of atopic dermatitis, urticaria, scleromyxedema, pyoderma gangrenosum, psoriasis, and pretibial myxedema

Jolles

Hughes

2006

International Immunopharmacology

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“…DUNTAS AND COOPER been localized to adipocytes, lymphocytes, fibroblasts (77,78), and erythrocytes (79). Recently, TSH has been found to regulate the secretion of interleukin-6 (IL-6) from human abdominal fat cells, and rhTSH has been shown to mediate IL-6 release from fat cells in thyroidectomized patients (80).…”

mentioning

confidence: 99%

Review on the Occasion of a Decade of Recombinant Human TSH: Prospects and Novel Uses

Duntas

Cooper

2008

Thyroid

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The introduction of recombinant human thyroid-stimulating hormone (rhTSH) almost a decade ago represents a remarkable achievement in the history of clinical thyroidology. rhTSH now contributes substantially to the diagnostic approach to thyroid cancer, offering a reliable and safe alternative to thyroid hormone withdrawal by avoiding the morbidity of hypothyroidism. Several recent studies have also demonstrated the efficacy of radioiodine ablation of thyroid remnants after preparation with rhTSH. Moreover, the use of rhTSH in this context is associated with a lower whole body exposure to radiation compared to thyroid hormone withdrawal. Although not approved officially, rhTSH-assisted treatment of locoregional or distant metastatic disease may be the treatment of choice for patients in whom hypothyroidism may be relatively contraindicated, such as the very young and the aged, although additional efficacy studies are necessary. The compound has also been shown to be useful in the treatment of nontoxic multinodular goiter, especially when the radioiodine uptake is low. Finally, rhTSH may prove to be useful in studying the functional reserve of thyroid in the aging process, as well as the putative role of the TSH receptor in extrathyroidal tissue, such as lymphocytes, osteocytes, and adipocytes.

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TSH and TSH Receptor Antibody-Binding Sites in Fibroblasts of Pretibial Myxedema Are Related to the Extracellular Domain of Entire TSH Receptor

Cited by 43 publications

References 0 publications

Thyrotropin Receptor Expression in Adrenal, Kidney, and Thymus

Thyrotropin Receptor Expression in Adrenal, Kidney, and Thymus

Use of IGIV in the treatment of atopic dermatitis, urticaria, scleromyxedema, pyoderma gangrenosum, psoriasis, and pretibial myxedema

Review on the Occasion of a Decade of Recombinant Human TSH: Prospects and Novel Uses

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