Tryptophan hydroxylase (TRH) loss of function mutations induce growth and behavioral defects in Daphnia magna

Rivetti, Claudia; Campos, Bruno; Piña, Benjamı́n; Raldúa, Demetrio; Kato, Yasuhiko; Watanabe, Hidenobu; Barata, Carlos

doi:10.1038/s41598-018-19778-0

Cited by 34 publications

(17 citation statements)

References 59 publications

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“…magna individuals chronically exposed to low levels of fluoxetine had higher amounts of serotonin in the brain and reproduced to a greater extent . Conversely, individuals having the tryptophan hydrolase gene mutated (which is the rate-limiting enzyme for serotonin biosynthesis) lacked serotonin and showed decreased growth and reproductive rates . In the present study, genes belonging to the dopaminergic and serotonin signaling pathways were particularly enriched in cluster B followed by clusters A and C in decreasing order.…”

Section: Discussionmentioning

confidence: 41%

Effects of Single and Combined Low Concentrations of Neuroactive Drugs on Daphnia magna Reproduction and Transcriptomic Responses

Fuertes

Campos

Rivetti

et al. 2019

Environ. Sci. Technol.

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Assessing the risk of neuroactive pharmaceuticals in the environment requires an understanding of their joint effects at low concentrations across species. Here, we assessed reproductive and transcriptional effects of single and ternary equi-effective mixture exposure to propranolol, diazepam, and carbamazepine on the crustacean Daphnia magna at environmentally relevant concentrations. The three compounds enhanced reproduction in adults and induced specific transcriptome changes in preadolescent individuals. Comparison of the results from single exposures to a ternary equi-effective mixture of the three compounds showed additive action. Transcriptomic analyses identified 3248 genes affected by at least one of the treatments, which were grouped into four clusters. Two clusters (1897 gene transcripts in total) behaved similarly, appearing either over-or under-represented relative to control, in all single and mixture treatments. The third and fourth clusters grouped genes differently transcribed upon exposure to diazepam and propranolol, respectively. Functional transcriptomics analysis indicated that the four clusters shared major deregulated signaling pathways implicated on energy, growth, reproduction, and neurologically related processes, which may be responsible for the observed reproductive effects. Thus, our study showed additive effects at the transcriptional and physiological level and provides a novel approach to the analysis of environmentally relevant mixtures of neuroactive compounds.

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Section: Discussionmentioning

confidence: 41%

Effects of Single and Combined Low Concentrations of Neuroactive Drugs on Daphnia magna Reproduction and Transcriptomic Responses

Fuertes

Campos

Rivetti

et al. 2019

Environ. Sci. Technol.

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“…Fluoxetine enhances brain serotonin activity in Daphnia (Campos et al, 2016), increases development and reproductive rates (Campos et al, 2012) and alters phototaxis behavior. Recent studies using knockout Daphnia individuals lacking serotonin showed that these animals had the opposite phenotype as those exposed to fluoxetine: animals matured latter, reproduced less and were more mobile than wild type animals (Rivetti et al, 2018). There is thus a neurofunctional link between fluoxetine, its pharmacological target serotonin and effects (life-history and behavioral changes).…”

Section: Discussionmentioning

confidence: 99%

Using a new high-throughput video-tracking platform to assess behavioural changes in Daphnia magna exposed to neuro-active drugs

Simão

Martínez‐Jerónimo

Blasco

et al. 2019

Science of The Total Environment

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One of the major challenges that faces today regulatory risk assessment is to speed up the way of assessing threshold sublethal detrimental effects of existing and new chemical products. Recently advances in imaging allows to monitor in real time the behaviour of individuals under a given stress. Light is a common stress for many different organisms. Fish larvae and many invertebrate species respond to light altering their behaviour. The water flea Daphnia magna as many other zooplanktonic species has a marked diel vertical phototactic swimming behaviour against light due to fish predation. The aim of this study was to develop a highthroughput image analysis to study changes in the vertical swimming behaviour to light of D. magna first reproductive adult females exposed to 0.1 and 1 µg/L of four psychiatric drugs: diazepam, fluoxetine, propranolol and carbamazepine during their entire life. Experiments were conducted using a new custom designed vertical oriented four 50 mL chamber device controlled by the Noldus software (Netherlands). Changes in speed, preferred area (bottom vs upper areas) and animal aggregation were analysed using groups of animals under consecutive periods of dark and apical light stimulus of different intensities. Obtained results indicated that light intensity increased the speed but low light intensities allowed to better discriminate individual responses to the studied drugs. The four tested drugs decreased the response of exposed organisms to light: individuals move less, were closer to the bottom and at low light intensities were closer each other. At high light intensities, however, exposed individuals were less aggregated. Propranolol, carbamazepine and fluoxetine were the compounds effecting most the behaviour. Our results indicated that psychiatric drugs at environmental relevant concentrations alter the vertical phototactic behaviour of D. magna individuals and that it is possible to develop appropriate high-throughput image analysis devices to measure those responses.

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“…(3) Role of hubgenes: on a functional level, recent advantages in molecular techniques now allow for a detailed testing of network structures to confirm if molecular cascades collapse when predicted hub-genes are modified via gene editing approaches (e.g., RNAi or CRISPr and Talen techniques 59-61 ).…”

Section: Discussionmentioning

confidence: 99%

Dissecting the transcriptomic basis of phenotypic evolution in an aquatic keystone grazer

Frisch

Becker

Wojewodzic

2019

Preprint

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26Cultural eutrophication is one of the largest threats to aquatic ecosystems with 27 devastating ecological and economic consequences. Although Daphnia, a keystone 28 grazer crustacean, can mitigate some negative effects of eutrophication in freshwater 29 habitats, it is itself affected by changes in nutrient composition. Previous studies have 30 shown evolutionary adaptation of Daphnia to environmental change, including high 31 phosphorus levels. While phosphorus is an essential macronutrient, it negatively 32affects Daphnia life history when present in excessive concentrations. Here, we map 33 weighted gene co-expression networks to phosphorus-related phenotypic traits in 34 centuries-old ancestral Daphnia and their modern descendants, contrasting pre-and 35 post-eutrophication environments. We find that evolutionary fine-tuning of 36 transcriptional responses is manifested at a basic (cellular) phenotypic level, and is 37 strongly correlated to an evolved plasticity of gene expression. At a higher phenotypic 38 level, this contributes to the maintenance of physiological homeostasis, and a robust 39 preservation of somatic phosphorus concentration. 40 41 3 Introduction 42Cultural eutrophication is one of the largest threats to aquatic ecosystems with 43 devastating ecological and economic effects 1 . Rising phosphorus (P) concentrations in 44 surface water result in high algal biomasses that pose a serious ecological threat to 45 aquatic habitats, impacting biodiversity, drinking water quality, recreational 46 resources, and fisheries 1-2 . One of the major P-sources of eutrophication is the use of 47 phosphate fertilizers, for which the global consumption has increased from 1 to 15 Mt 48 P year -1 in the 20th century 3 . Vital ecosystem services provided by Daphnia, a 49 keystone herbivore crustacean in freshwater habitats, can effectively mitigate some of 50 these negative impacts by decimating phytoplankton biomass 4 . Although P is a vital 51 element for many cellular components, including RNA, DNA and ATP, excessive P-52 concentrations typical of eutrophication can negatively affect somatic and population 53 growth of Daphnia 5-6 . In addition, algal blooms often involve toxin-producing 54 cyanobacteria that can negatively affect the growth rate of Daphnia, although local 55 adaptation to these toxins has been observed 7-9 . Insights from resurrection ecology 10 56 suggest that Daphnia can adapt to changing environmental conditions, such as climate 57 change 11 , anthropogenic stressors 12 , toxic algae 13 , and phosphorus supply 14 . Although 58 previous analyses have examined the functional genome underlying Daphnia 59 phenotypes (e.g. [15][16][17][18][19] ), the link between phenotypic adaptation and the evolution of 60 gene expression reaction norms in this keystone species is poorly understood. 61Adaptation to environmental change has been associated with divergent gene 62 expression patterns 20 and the maintenance or evolution of gene expression reaction 63 norms [21][22] . However, directly linking...

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Tryptophan hydroxylase (TRH) loss of function mutations induce growth and behavioral defects in Daphnia magna

Cited by 34 publications

References 59 publications

Effects of Single and Combined Low Concentrations of Neuroactive Drugs on Daphnia magna Reproduction and Transcriptomic Responses

Effects of Single and Combined Low Concentrations of Neuroactive Drugs on Daphnia magna Reproduction and Transcriptomic Responses

Using a new high-throughput video-tracking platform to assess behavioural changes in Daphnia magna exposed to neuro-active drugs

Dissecting the transcriptomic basis of phenotypic evolution in an aquatic keystone grazer

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