Tryptic Soy Bile-Kanamycin Test for the Identification of Bacteroides fragilis

Vargo, Valerie; Korzeniowski, Myroslawa; Spaulding, Earle H.

doi:10.1128/aem.27.3.480-483.1974

Cited by 11 publications

(11 citation statements)

References 11 publications

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“…This information could be used together with information on relative sensitivity to antibiotics, etc. (23), for development of truly selective methods of isolation and enumeration. The minimal medium has value in genetic studies and can be useful in systematic studies for separation of B. fragilis from many other species in the family Bacteriodaceae.…”

Section: Discussionmentioning

confidence: 99%

Nutritional Features of Bacteroides fragilis subsp. fragilis

Varel

Bryant

1974

Applied Microbiology

125

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Studies of three reference strains of Bacteroides fragilis subsp. fragilis showed that they grow well in a minimal defined medium containing glucose, hemin, vitamin B,2, minerals, bicarbonate-carbon dioxide buffer, NHCl, and sulfide. The vitamin B,2 requirement of 0.1 ng/ml was replaced with 7.5 ,g of methionine. Cysteine or sulfide was an excellent source of sulfur, thioglycolate was a poor source, and thiosulfate, methionine, P-mercaptoethanol, dithiothreitol, sulfate, or sulfite did not serve as sole sources of sulfur. Neither single amino acids, nitrate, urea, nor a complex mixture of L-amino acids or peptides effectively replaced ammonia as the nitrogen source. Comparative studies with a few strains of other subspecies of B. fragilis including B. fragilis subsp. vulgatus, B. fragilis subsp. thetaiotaomicron, and B. fragilis subsp. distasonis indicate that they exhibit similar growth responses in the minimal medium. A single strain of B. fragilis subsp. ovatus required other materials. The results indicate the great biosynthetic ability of these organisms and suggest that, in their ecological niche within the large intestine, many nutrients such as amino acids are in very low supply, whereas materials such as ammonia, heme, and vitamin B,2, or related compounds, must be available during much of the time.

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Section: Discussionmentioning

confidence: 99%

Nutritional Features of Bacteroides fragilis subsp. fragilis

Varel

Bryant

1974

Applied Microbiology

125

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“…MacConkey lactose bile salts agar for the isolation of organisms of the colon-typhoid group originally contained 5 mg of sodium glycocholate per ml (3,9). TS bile agar, used for the identification of the anaerobe Bacteroides fragilis, consists of tryptic soy agar containing 2% oxgall equivalent to 20% bile (26), and the effects of bile and deoxycholate on some gram-negative anaerobes have been investigated (21). Thus, the lysis of some bacteria with bile acids has long been known.…”

Section: Discussionmentioning

confidence: 99%

Lysis of Halobacteria in Bacto-Peptone by Bile Acids

Kamekura

Oesterhelt

Wallace

et al. 1988

Appl Environ Microbiol

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All tested strains of halophilic archaebacteria of the genera Halobacterium, Haloarcula, Haloferar, and Natronobacterium lysed in 1% Bacto-Peptone (Difco) containing 25% NaCl, whereas no lysis was observed with other strains belonging to archaebacteria of the genera Halococcus, Natronococcus, and Sulfolobus, methanogenic bacteria, and moderately halophilic eubacteria. Substances in Bacto-Peptone which caused lysis of halobacteria were purified and identified as taurocholic acid and glycocholic acid. High-performance liquid chromatography analyses of peptones revealed that Bacto-Peptone contained nine different bile acids, with a total content of 9.53 mg/g, whereas much lower amounts were found in Peptone Bacteriological Technical (Difco) and Oxoid Peptone. Different kinds of peptones can be used to distinguish halophilic eubacteria and archaebacteria in mixed cultures from hypersaline environments.

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“…BFG strains were separated from other anaerobic gramnegative rods by kanamycin resistance (1,000-Mg disc) and growth on trypticase soy agar containing 20% bile. 28 Speciation was based on indole production and fermentation of glucose, trehalose, mannitol, and rhamnose. 25 Organisms not tested immediately were stored at -60°C in fetal calf serum.…”

Section: Bacterial Strains and Growth Conditionsmentioning

confidence: 99%

Demonstration of Bacteroides Capsules by Light Microscopy and Ultrastructural Cytochemistry

Strohm

Payne

Ryan

1983

American Journal of Clinical Pathology

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Forty-six anaerobic gram-negative bacilli, including 26 members of the Bacteroides fragilis group (BFG), were examined for capsules by the India ink technic. Thirty-five were encapsulated, including all the BFG strains. As a follow-up, seven of these isolates and two previously studied reference strains (B. fragilis ATCC 23745 and Bacteroides vulgatus ATCC 8482) were examined for capsules by ultrastructural cytochemistry. Using the periodic acid thiocarbohydrazide silver proteinate (PATCSP) method of Thiéry, all the BFG examined were encapsulated. In addition to the reference strains, this included one strain of B. fragilis and four of Bacteroides thetaiotaomicron. One non-BFG strain showed no capsular material. Differences between these results and those reported previously with the ruthenium red technic may reflect species differences in the chemical composition of Bacteroides capsules.

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Tryptic Soy Bile-Kanamycin Test for the Identification of Bacteroides fragilis

Cited by 11 publications

References 11 publications

Nutritional Features of Bacteroides fragilis subsp. fragilis

Nutritional Features of Bacteroides fragilis subsp. fragilis

Lysis of Halobacteria in Bacto-Peptone by Bile Acids

Demonstration of Bacteroides Capsules by Light Microscopy and Ultrastructural Cytochemistry

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