Tryptic Soy Bile-Kanamycin Test for the Identification of
            <i>Bacteroides fragilis</i>

All tested strains of halophilic archaebacteria of the genera Halobacterium, Haloarcula, Haloferar, and Natronobacterium lysed in 1% Bacto-Peptone (Difco) containing 25% NaCl, whereas no lysis was observed with other strains belonging to archaebacteria of the genera Halococcus, Natronococcus, and Sulfolobus, methanogenic bacteria, and moderately halophilic eubacteria. Substances in Bacto-Peptone which caused lysis of halobacteria were purified and identified as taurocholic acid and glycocholic acid. High-performance liquid chromatography analyses of peptones revealed that Bacto-Peptone contained nine different bile acids, with a total content of 9.53 mg/g, whereas much lower amounts were found in Peptone Bacteriological Technical (Difco) and Oxoid Peptone. Different kinds of peptones can be used to distinguish halophilic eubacteria and archaebacteria in mixed cultures from hypersaline environments.

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Nutritional Features of Bacteroides fragilis subsp. fragilis

Varel

Bryant

1974

Appl Microbiol

168

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Studies of three reference strains of Bacteroides fragilis subsp. fragilis showed that they grow well in a minimal defined medium containing glucose, hemin, vitamin B 12 , minerals, bicarbonate-carbon dioxide buffer, NH 4 Cl, and sulfide. The vitamin B 12 requirement of 0.1 ng/ml was replaced with 7.5 μg of methionine. Cysteine or sulfide was an excellent source of sulfur, thioglycolate was a poor source, and thiosulfate, methionine, β-mercaptoethanol, dithiothreitol, sulfate, or sulfite did not serve as sole sources of sulfur. Neither single amino acids, nitrate, urea, nor a complex mixture of L-amino acids or peptides effectively replaced ammonia as the nitrogen source. Comparative studies with a few strains of other subspecies of B. fragilis including B. fragilis subsp. vulgatus, B. fragilis subsp. thetaiotaomicron , and B. fragilis subsp. distasonis indicate that they exhibit similar growth responses in the minimal medium. A single strain of B. fragilis subsp. ovatus required other materials. The results indicate the great biosynthetic ability of these organisms and suggest that, in their ecological niche within the large intestine, many nutrients such as amino acids are in very low supply, whereas materials such as ammonia, heme, and vitamin B 12 , or related compounds, must be available during much of the time.

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Tryptic Soy Bile-Kanamycin Test for the Identification of Bacteroides fragilis

Cited by 9 publications

References 10 publications

Effect of selective media on recovery of obligately anaerobic gram-negative rods from human faeces

Effect of selective media on recovery of obligately anaerobic gram-negative rods from human faeces

Lysis of Halobacteria in Bacto-Peptone by Bile Acids

Nutritional Features of Bacteroides fragilis subsp. fragilis

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