Trypsin inhibitor from tamarindus indica L. seeds reduces weight gain and food consumption and increases plasmatic cholecystokinin levels

Ribeiro, Joycellane Alline do Nascimento Campos; Serquiz, Alexandre Coelho; Silva, Priscila Fabíola dos Santos; Barbosa, Patrícia Batista Barra Medeiros; Sampaio, Tarcísio Bruno Montenegro; Araújo, Raimundo Fernandes de; Oliveira, Adeliana S.; Machado, Richele J. A.; Maciel, Bruna Leal Lima; Uchôa, Adriana Ferreira; Santos, Elisama Vieira dos; Morais, Ana Heloneida de Araújo

doi:10.6061/clinics/2015(02)11

Cited by 42 publications

(73 citation statements)

References 28 publications

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“…Recent research by our group demonstrated that an isolated trypsin inhibitor from Tamarindus indica L. seed (TTI) presents anti-obesity and anti-inflammatory activities [28, 29]. Ribeiro et al [28] analyzed the influence of TTI in an experimental model of satiety and found that the inhibitor was able to reduce food intake and weight gain of eutrophic animals, possibly by raising plasma CCK.…”

Section: Introductionmentioning

confidence: 99%

Satietogenic Protein from Tamarind Seeds Decreases Food Intake, Leptin Plasma and CCK-1r Gene Expression in Obese Wistar Rats

et al. 2018

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Objective: This study evaluated the effect of a protein, the isolated Trypsin Inhibitor (TTI) from Tamarindus indica L. seed, as a CCK secretagogue and its action upon food intake and leptin in obese Wistar rats. Methods: Three groups of obese rats were fed 10 days one of the following diets: Standard diet (Labina®) + water; High Glycemic Index and Load (HGLI) diet + water or HGLI diet + TTI. Lean animals were fed the standard diet for the 10 days. Food intake, zoometric measurements, plasma CCK, plasma leptin, relative mRNA expression of intestinal CCK-related genes, and expression of the ob gene in subcutaneous adipose tissue were assessed. Results: TTI decreased food intake but did not increase plasma CCK in obese animals. On the other hand, TTI treatment decreased CCK-1R gene expression in obese animals compared with the obese group with no treatment (p = 0.027). Obese animals treated with TTI presented lower plasma leptin than the non-treated obese animals. Conclusion: We suggest that TTI by decreasing plasma leptin may improve CCK action, regardless of its increase in plasma from obese rats, since food intake was lowest.

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Section: Introductionmentioning

confidence: 99%

Satietogenic Protein from Tamarind Seeds Decreases Food Intake, Leptin Plasma and CCK-1r Gene Expression in Obese Wistar Rats

et al. 2018

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“…The low body weight gain in the 1% OM diet group may have caused pancreatic hypersecretion leading to energy metabolism-promoting actions. A suppression of body weight gain also has been reported for other trypsin inhibitors (23,24). Thus, it was suggested that the low body weight gain in the OIT group caused the trypsin inhibition of OM.…”

Section: Discussionmentioning

confidence: 77%

Intake of Diet Including 1% Ovomucoid for 4 Weeks Induces Oral Desensitization in Ovomucoid-Specific Allergic Mouse Model

Maeta

Sakamoto

Yuki

et al. 2017

J Nutr Sci Vitaminol

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SummaryWe propose a new oral immunotherapy (OIT) method that includes a small amount of a food allergen in the diet. However, it is not clear whether this method will induce oral desensitization and immune tolerance. Therefore, we investigated the therapeutic effectiveness using a 1% food allergen diet in an allergic mouse model. C3H/HeJ mice were sensitized to ovomucoid (OM) in alum four times at 12-d intervals. Sensitized mice were divided into two groups: the OIT group (19% casein diet with 1% OM) and the non-treated group (20% casein diet without OM). The non-sensitized mice served as the non-allergy group. The OIT treatment was performed for 4 wk. To assess desensitization and immune tolerance, we performed oral and intraperitoneal OM challenges, assessed vascular permeability of the dorsal skin, and measured allergic biomarkers. The OIT group exhibited significantly lower oral symptom scores and vascular permeability than the non-treated group, but the two groups did not differ in intraperitoneal allergy symptom scores. Furthermore, the OIT group had significantly higher OM-specific IgA levels in their plasma than the non-treated group. However, the plasma levels of OM-specific IgE, IgG1, and IgG2a were not significantly different between the OIT and the non-treated groups. These results suggest that the proposed OIT using an OM-supplemented diet may induce desensitization, but not immune tolerance, in an OM allergic mouse model. Key Words oral immunotherapy, ovomucoid, food allergy, diet Globally, 240-550 million people may suffer from food allergies (1). Recently, the prevalence of food allergies has increased by 18% among children under 18 y of age (2, 3). Dietary avoidance of specific allergens is recommended as the first-line approach in the management of food allergy (4). In Japan, egg allergy is the most prevalent food hypersensitivity among the pediatric population, exceeding cow's milk allergy (4). A major cause of egg allergy is ovomucoid (OM), which constitutes approximately 11% of egg white protein (5, 6). Oral immunotherapy (OIT) is a new treatment for food allergies (4, 7). This treatment involves inducing desensitization through continuous food-allergen intake. However, according to the Japanese pediatric guidelines for food-allergy treatment, OIT is not often recommended because of the risk of inducing the allergic symptoms during the treatment (4). Moreover, current data are inadequate to establish proper OIT protocols, such as allergen dose and timing, need for escalation, therapy duration, and routes of administration.There are some reports of the OIT protocol in an allergic mouse model (8)(9)(10)(11)(12). We propose a new OIT regimen that involves mixing a small amount of allergen powder in the diet. Some merits of this OIT method are: 1) the allergen dose is divided, 2) allergen doses ingested can be easily adjusted, and 3) the taste and smell of the allergen is masked. Few reports exist regarding the relationship between oral desensitization and an allergencontaining diet in allergic ...

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“…Ribeiro et al; 21 Serguiz et al; 22 Lima et al; 23 Carvalho et al 24 também isolaram inibidores de tripsina do partido da F2.…”

Section: Figuraunclassified

“…37 Para a nutrição, diferente do estigma que os relacionam aos efeitos antinutricionais, esses inibidores possuem papel heterólogo e bioativo no processo de digestão de proteínas, apresentando efeito no controle da saciedade com consequente redução de peso em modelo experimental, tendo em vista que, a partir da diminuição da atividade de tripsina livre e consequente redução da quebra de proteínas e peptídeos, há elevação da concentração do hormônio sacietogênico CCK. 21,22,[38][39][40][41][42] A detecção da atividade antitríptica e o isolamento de um inibidor de tripsina em sementes de chia são achados inéditos, uma vez que não foi encontrado, na busca da literatura científica realizada nas bases de dados, estudos anteriores que tenham analisado a presença desses inibidores. As proteínas da chia têm sido avaliadas quanto possíveis atividades biológicas, 43,44 sendo relatada inclusive a presença de peptídeos com ação inibitória sobre a enzima conversora de angiotensina.…”

Section: Figuraunclassified

“…18 Porém, quanto aos inibidores enzimáticos, tem-se observado um potencial terapêutico em sua utilização heteróloga para o tratamento de alguns estados de doença. [19][20][21][22][23][24] Embora os inibidores de tripsina estejam presentes naturalmente no reino vegetal, nas sementes de chia não existe relato na literatura sobre tal presença. Além disso, é importante investigar se esses inibidores comuns em sementes, também se apresentam e se mantêm nos seus produtos, como na farinha.…”

Section: Introductionunclassified

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Atividade Antitríptica Em Semente E Produto Alimentício De Chia (Salvia Hispanica L.)

Souza

Nascimento

Lima

et al. 2017

DEMETRA

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Anti-tryptic activity in seed and food product of chia (Salvia hispanica L.) ResumoObjetivo: Este estudo objetiva detectar a atividade antitríptica em semente e farinha comerciais de chia e isolar um inibidor de tripsina presente na semente. Metodologia: Foram realizados extração de proteínas, fracionamento com sulfato de amônio, cromatografia de afinidade, ensaio de inibição sobre tripsina, quantificação de proteínas e eletroforese em gel de poliacrilamida desnaturante. Resultados e discussão: A partir desses experimentos, detectou-se a atividade antitríptica no pico proteico retido na coluna de afinidade (RT), da farinha e semente, apresentando 51% e 64%, respectivamente. Porém, não foram verificadas grandes quantidades de proteínas solúveis no RT, tanto da semente quanto da farinha, quando comparadas ao extrato bruto e frações proteicas. O inibidor de tripsina de semente foi isolado, indicando massa molecular estimada em, aproximadamente, 14,4 kDa. Conclusões: Diante dos resultados relativos à detecção da atividade antitrípica em semente e farinha comercial de chia, e isolamento do inibidor na semente, faz-se necessário o aprofundamento nas pesquisas acerca dessa molécula quanto as suas propriedades bioativas e segurança do consumo.Palavras-chaves: Farinha. Sementes. Inibidores de Proteases. Tripsina. AbstractObjective: This study aimed to detect anti-tryptic activity in commercial chia seed and flour and to isolate a trypsin inhibitor present in the seed. Methodology: Protein extraction, ammonium

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Trypsin inhibitor from tamarindus indica L. seeds reduces weight gain and food consumption and increases plasmatic cholecystokinin levels

Cited by 42 publications

References 28 publications

Satietogenic Protein from Tamarind Seeds Decreases Food Intake, Leptin Plasma and <b><i>CCK-1r</i></b> Gene Expression in Obese Wistar Rats

Satietogenic Protein from Tamarind Seeds Decreases Food Intake, Leptin Plasma and <b><i>CCK-1r</i></b> Gene Expression in Obese Wistar Rats

Intake of Diet Including 1% Ovomucoid for 4 Weeks Induces Oral Desensitization in Ovomucoid-Specific Allergic Mouse Model

Atividade Antitríptica Em Semente E Produto Alimentício De Chia (Salvia Hispanica L.)

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