Trypsin inhibitor activity in mature tobacco and tomato plants is mainly induced locally in response to insect attack, wounding and virus infection

Jongsma, Maarten A.; Bakker, Petra L.; Visser, Bert; Stiekema, Willem J.

doi:10.1007/bf00206288

Cited by 88 publications

(75 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To evaluate if the substantially elevated herbivore resistance of irJAZh plants was due to higher levels of toxic defense metabolites in these plants, we examined trypsin protease inhibitor (TPI) activity (Jongsma et al, 1994(Jongsma et al, , 1995Habib and Fazili, 2007) and the accumulation of the secondary metabolites nicotine (Shoji et al, 2000;Steppuhn et al, 2004) and 17-hydroxygeranyllinalool diterpene glycosides (DTGs; Jassbi et al, 2008;Heiling et al, 2010) in W+OS-elicited irJAZh and wild-type plants (Fig. 6).…”

Section: Silencing Of Najazh Alters the Expression Of Other Jaz Genesmentioning

confidence: 99%

“…plants produce nicotinic alkaloids to ward off attack from feeding herbivores (Baldwin et al, 1997;Wink and Roberts, 1998;Shoji et al, 2000;Steppuhn et al, 2004). In addition, most plants produce protease inhibitors in response to herbivory, which inhibit proteolysis and negatively affect the digestibility of ingested plant material in insect guts (Jongsma et al, 1994(Jongsma et al, , 1995Koiwa et al, 1997;Zavala et al, 2004a;Habib and Fazili, 2007;Hartl et al, 2010). Green leaf volatiles (GLVs) and volatile organic compounds (VOCs) constitute another important plant defense mechanism to attract predators of herbivores; this strategy is also known as indirect plant defense (Halitschke et al, 2000;Kessler and Baldwin, 2001;Baldwin et al, 2002;Allmann and Baldwin, 2010).…”

mentioning

confidence: 99%

See 1 more Smart Citation

NaJAZh Regulates a Subset of Defense Responses against Herbivores and Spontaneous Leaf Necrosis inNicotiana attenuataPlants

Baldwin

Gàlis

2012

Plant Physiology

View full text Add to dashboard Cite

The JASMONATE ZIM DOMAIN (JAZ) proteins function as negative regulators of jasmonic acid signaling in plants. We cloned 12 JAZ genes from native tobacco (Nicotiana attenuata), including nine novel JAZs in tobacco, and examined their expression in plants that had leaves elicited by wounding or simulated herbivory. Most JAZ genes showed strong expression in the elicited leaves, but NaJAZg was mainly expressed in roots. Another novel herbivory-elicited gene, NaJAZh, was analyzed in detail. RNA interference suppression of this gene in inverted-repeat (ir)JAZh plants deregulated a specific branch of jasmonic acid-dependent direct and indirect defenses: irJAZh plants showed greater trypsin protease inhibitor activity, 17-hydroxygeranyllinalool diterpene glycosides accumulation, and emission of volatile organic compounds from leaves. Silencing of NaJAZh also revealed a novel cross talk in JAZ-regulated secondary metabolism, as irJAZh plants had significantly reduced nicotine levels. In addition, irJAZh spontaneously developed leaf necrosis during the transition to flowering. Because the lesions closely correlated with the elevated expression of programmed cell death genes and the accumulations of salicylic acid and hydrogen peroxide in the leaves, we propose a novel role of the NaJAZh protein as a repressor of necrosis and/or programmed cell death during plant development.

show abstract

Section: Silencing Of Najazh Alters the Expression Of Other Jaz Genesmentioning

confidence: 99%

mentioning

confidence: 99%

NaJAZh Regulates a Subset of Defense Responses against Herbivores and Spontaneous Leaf Necrosis inNicotiana attenuataPlants

Baldwin

Gàlis

2012

Plant Physiology

View full text Add to dashboard Cite

show abstract

“…Harvested leaves were ground in liquid nitrogen individually and total protein was extracted using 2 mL of protein extraction buffer (Jongsma et al, 1994) per milligram plant tissue, followed by vortexing and centrifugation as described in Van Dam et al (2001). Protein content of the samples was determined in technical triplicate using the method of Bradford (1976) with an IgG-based (Sigma) standard curve.…”

Section: Tpi Assaymentioning

confidence: 99%

Systemin in Solanum nigrum. The Tomato-Homologous Polypeptide Does Not Mediate Direct Defense Responses

Schmidt

Baldwin

2006

Plant Physiology

View full text Add to dashboard Cite

We extend Ryan's seminal work on the 18-amino acid polypeptide systemin in tomato's (Solanum lycopersicum) systemic wound response to the closely related solanaceous species Solanum nigrum. We compared wild-type plants to plants transformed with an inverted repeat prosystemin construct (IRSys) to silence the expression of the endogenous S. nigrum prosystemin gene. In wild-type plants elicited with wounding 1 oral secretions from Manduca sexta larvae, trypsin-proteinase inhibitors (TPIs) accumulated even though prosystemin transcripts were down-regulated. Neither reducing the endogenous systemin levels by RNAi nor complementing the plants with systemin by exogenously supplying the polypeptide through excised stems significantly increased TPI activity, indicating that systemin and TPIs are not correlated in S. nigrum. The performance of two herbivore species from two feeding guilds, M. sexta larvae and Myzus persicae nicotianae, did not differ between wild-type and IRSys plants, demonstrating that varying endogenous systemin levels do not alter the direct defenses of S. nigrum. Field experiments with wild-type and IRSys plants and the flea beetle Epitrix pubescens supported these glasshouse data. That levels of oral secretion-elicited jasmonic acid did not differ between wild-type and IRSys plants suggests that systemin is unlikely to mediate jasmonate signaling in S. nigrum as it does in tomato. We conclude that the tomato-homologous polypeptide does not mediate direct defense responses in S. nigrum.

show abstract

“…The induced PIs represent either local [7,17] or systemic [18][19][20][21] responses, and either age-dependent [22] or organ-specific [8,18] traits. Application of chemical signals, such as jasmonic acid (JA), abscisic acid (ABA) and ethylene or salicylic acid (SA) can manipulate the production of PIs in plants [13,14,23,24], but the effects of each compound differ for different PIs.…”

mentioning

confidence: 99%

Salicylic acid and ethylene signaling pathways are involved in production of rice trypsin proteinase inhibitors induced by the leaf folder Cnaphalocrocis medinalis (Guenée)

Wang

Zhou

et al. 2011

Chin. Sci. Bull.

View full text Add to dashboard Cite

The roles of signaling pathways in the production of trypsin proteinase inhibitors (TrypPIs) in rice infested by the leaf folder (LF) Cnaphalocrocis medinalis were studied. Infestation by LF increased TrypPI levels in the leaves of rice plants at the tillering, booting and flowering stages but decreased TrypPI levels at the ripening stage; TrypPI levels in rice stems did not increase at any developmental stage. Infestation by LF at the tillering stage systemically increased TrypPI levels in leaves but not in stems; it also enhanced salicylic acid (SA) levels in leaves and stems, and the ethylene level released from plants. However, LF infestation did not increase JA concentrations. Exogenous application of SA or ethylene enhanced TrypPI levels in the leaves and stems of plants at the tillering stage, whereas treatment with both SA and ethylene induced lower levels of TrypPIs than treatment with SA or ethylene alone, suggesting an antagonistic effect of SA and ethylene on TrypPIs induction. The results suggest that both SA and ethylene signaling pathways are involved in the production of TrypPIs in rice induced by LF; moreover, the antagonistic effect of SA and ethylene may explain the changes in TrypPI levels seen at different plant developmental stages and in different organs.rice, Cnaphalocrocis medinalis, jasmonic acid, salicylic acid, ethylene, trypsin proteinase inhibitor, herbivore-induced defense response Citation:Wang X, Hu L C, Zhou G X, et al. Salicylic acid and ethylene signaling pathways are involved in production of rice trypsin proteinase inhibitors induced by the leaf folder Cnaphalocrocis medinalis (Guenée).

show abstract

Trypsin inhibitor activity in mature tobacco and tomato plants is mainly induced locally in response to insect attack, wounding and virus infection

Cited by 88 publications

References 23 publications

NaJAZh Regulates a Subset of Defense Responses against Herbivores and Spontaneous Leaf Necrosis inNicotiana attenuataPlants

NaJAZh Regulates a Subset of Defense Responses against Herbivores and Spontaneous Leaf Necrosis inNicotiana attenuataPlants

Systemin in Solanum nigrum. The Tomato-Homologous Polypeptide Does Not Mediate Direct Defense Responses

Salicylic acid and ethylene signaling pathways are involved in production of rice trypsin proteinase inhibitors induced by the leaf folder Cnaphalocrocis medinalis (Guenée)

Contact Info

Product

Resources

About