Trypsin from zebra blenny (Salaria basilisca) viscera: Purification, characterisation and potential application as a detergent additive

Study on enzymes of industrial interest in digestive viscera: Greater amberjack (Seriola dumerili)

“…Optimum pH of trypsin is consistent with that reported by Ben Khaled et al [10], Freitas-Junior et al [8], Ktari et al [11] and Sila et al [12] for the fish species sardinelle S. aurita, pirarucu A. gigas, zebra blenny S. basilisca and barbel B. callensis, respectively, indicating an optimum pH range between 8.0 and 10. In this study, peak activity of trypsin was found in the pH 9.0.…”

“…The results of in vitro exposure to metal ions for trypsin are in accordance with those obtained for zebra blenny Salaria basilisca [11]. Costa et al [1] [27] for crucian carp C.…”

“…The results for trypsin showed similar values to that of alkaline trypsin from silver mojarra D. rhombeus [7], pirarucu A. gigas [8] and crevalle jack C. hippos [1]; while chymotrypsin values diverged from that reported by Castillo-Yañez et al [25]. The effect of temperature on the activity of trypsin in this work was similar to that of Brownstripe red snapper Lutjanus vitta [26] and zebra blenny Salaria basilisca [11]. The loss of activity presumably was due to heat treatment.…”

“…The activity of the trypsin from crude extract tested in the present study was higher than that from crude extract of Japanese sea bass Lateolabrax japonicus (0.30 IU/mg) [20], zebra blenny Salaria basilisca (0.12 IU/mg) [11], pirarucu A. gigas (0.37 IU/mg [8], tropical gar A. tropicus (0.000 006 IU/mg) [13] and hybrid catfish (Clarias microcephalus x Clarias gariepinus) (0.18 IU/mg) [21].…”

“…Digestive serine proteases have already been extracted from the guts of different types of fishes, such as trypsin from the Silver mojarra Diapterus rhombeus [7], sardinelle Sardinella aurita [10], zebra blenny Salaria basilisca [11], barbel Barbus callensis [12], pirarucu Arapaima gigas [8], crevalle jack Caranx hippos [1], tropical gar Atractosteus tropicus [13] and Nile tilapia Oreochromis niloticus [14]; and chymotrypsin from the cuttlefish Sepia officinalis [15], monterey sardine Sardinops sagax caerulea [16], tropical gar A. tropicus [13] and Nile tilapia O. niloticus [9].…”