Trypsin and Papain Covalently Coupled to Porous Glass: Preparation and Characterization

Abstract

Trypsin and papain have been covalently linked to porous glass particles. The resulting insolubilized enzymes show increased thermal stability and can be employed for extended periods of time without loss of activity.

Biologically Active Catecholamines Covalently Bound to Glass Beads

“…The hydrochloride salts of L-epinephrine (Sigma), DL-isoproterenol (Sigma), propranolol (Ayerst Laboratories) and L-norepinephrine (Sigma) were covalently attached to aryl amine glass (Corning) by a procedure similar to that of Weetall (4 Single beating heart cells from chicken embryos of from 5-18 days were obtained in culture at a density of 4000 cells/mm2. Cells were cultured in 30-ml Falcon flasks in accordance with the dissection and disaggregation procedures described by DeHaan (5).…”

A new method for coupling glucose dehydrogenase to glass tubes activated with titanium tetrachloride

“…However, many studies on enzymes immobilized on controlled-pore glass have appeared in the literature [2][3][4][5][6][7][8]. In these methods the controlled-pore glasses were used in particle form of the size of 40-80 mesh with pore diam.…”

Améliorations des caractéristiques de la présure fixée sur verre poreux

“…[11,7,12] Les billes de verre sont agitées pendant 16 h dans 10 ml de toluène contenant 10 p. 100 de v-aminopropyltriéthoxysilane.…”

“…Par la méthode de fixation utilisant la diazotation sur billes de verre silanisées [11,7,12], nous avons pu immobiliser la présure L'activité résiduelle est en moyenne de 11,7p. 100 et le rendement de fixation est de 96,5 p. 100.…”