Trypanosomiasis in camels: Its detection by complement fixation

Schoening, H. W.

doi:10.1093/infdis/34.6.608

Cited by 2 publications

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“…In order to improve diagnostic efficiency, several serological tests have been used to detect T. evansi antibodies in the camel. These include the complement fixation test (Schoening, 1924), indirect fluorescent antibody assays (Luckins et al 1979) and indirect haemagglutination tests (Wilson et al 1983).…”

Section: P F Rae and Othersmentioning

confidence: 99%

Evaluation of enzyme immunoassays in the diagnosis of camel (Camelus dromedarius) trypanosomiasis: a preliminary investigation

Rae¹,

Thrusfield²,

Higgins³

et al. 1989

Epidemiol. Infect.

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SUMMARYThree enzyme immunoassays were used for the serodiagnosis of Trypan8osoma evansi in camels in the Sudan in order to evaluate their ability to discriminate between infected and non-infected animals. Two assays were used for the detection of trypanosomal antibodies, one using specific anti-camel IgG conjugate and another using a non-specific Protein A conjugate. The third assay detected the presence of trypanosomal antigens using anti-T. evansi antibodies in a double antibody sandwich assay. Inspection of the frequency distribution of assay results suggested that the ELISA for circulating trypanosomal antibodies using specific antisera and the ELISA for circulating antigens can distinguish between noninfected camels and infected camels exhibiting patent infections or not. The ELISA using Protein A conjugate to bind non-specifically to camel immunoglobulin did not appear to discriminate between infected and non-infected animals.

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Section: P F Rae and Othersmentioning

confidence: 99%

Evaluation of enzyme immunoassays in the diagnosis of camel (Camelus dromedarius) trypanosomiasis: a preliminary investigation

Rae¹,

Thrusfield²,

Higgins³

et al. 1989

Epidemiol. Infect.

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Serodiagnosis of infection withTrypanosoma evansi in camels in the Sudan

Luckins

Boid

Rae

et al. 1979

Trop Anim Health Prod

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Five diagnostic tests for infection with Trypanosoma evansi have been compared in groups of camels experimentally infected or exposed to natural infection in the Sudan. The correlation of positive results obtained by assays of IgM levels, the mercuric chloride test and the formol gel test with the presence of active infection was unsatisfactory, but there was a good correlation between results obtained using IFAT and ELISA and proven infection. Sera from a high proportion of apparently uninfected camels from endemic areas gave positive reactions with all 5 tests, possibly indicating inadequate parasitological diagnosis or persistence of antibody after unsatisfactory chemotherapy. It was concluded that serological tests using trypanosomal antigens to detect antibodies were more sensitive for diagnosis than indirect tests based on raised euglobulin levels. Serodiagnostic tests may therefore have a place in future programmes for surveillance and control of T. evansi infections in camels.

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Trypanosomiasis in camels: Its detection by complement fixation

Cited by 2 publications

References 0 publications

Evaluation of enzyme immunoassays in the diagnosis of camel (Camelus dromedarius) trypanosomiasis: a preliminary investigation

Evaluation of enzyme immunoassays in the diagnosis of camel (Camelus dromedarius) trypanosomiasis: a preliminary investigation

Serodiagnosis of infection withTrypanosoma evansi in camels in the Sudan

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