Trypanosoma rangeli: un protozoo infectivo y no patógeno para el humano que contribuye al entendimiento de la transmisión vectorial y la infección por Trypanosoma cruzi, agente causal de la enfermedad de Chagas

Vallejo, Gustavo Adolfo Calderón; Suárez, Yazmín; Olaya, Jenny Lorena; Gutiérrez, Sneider Alexander; Carranza, Julio César

doi:10.18257/raccefyn.143

Cited by 22 publications

(35 citation statements)

References 47 publications

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“…It is interesting to note that T. rangeli stocks from Rhodnius spp. (Pallescens lineage) were identified in the KP1(-) group and lineage C, an association that has been proposed (Vallejo et al 2015). However, the reference strain R1625 (human origin) was classified in the lineage C (Maia-da-Silva et al 2007), although being KP1(+), as reported by Sincero et al (2015) and confirmed by our results.…”

Section: Biochemical and Molecular Analysessupporting

confidence: 89%

“…As far we know, KP1(+) and KP1(-) T. rangeli strains have not yet been compared by their morphological and biological features, and the present paper evidenced other peculiarities of the SC-58 and SC-61 isolates from P. dasythrix from Santa Catarina Island. Recent molecular studies (Sincero et al 2015) point to a subdivision within the KP1(-) group, which corroborate the uniqueness of these isolates, besides others from wild Panstrongylus megistus from the same region (Vallejo et al 2002(Vallejo et al , 2015. This may reflect a process of speciation within T. rangeli, as advanced by some authors (Macedo et al 1993, closely associated to the vector lineages (Machado et al 2001;Vallejo et al 2002Vallejo et al , 2015Urrea et al 2005Urrea et al , 2011Maia-da-Silva et al 2007;Salazar-Antón et al 2009;Barreto-Santana et al 2015;Sincero et al 2015).…”

Section: Discussionsupporting

confidence: 61%

“…Recent molecular studies (Sincero et al 2015) point to a subdivision within the KP1(-) group, which corroborate the uniqueness of these isolates, besides others from wild Panstrongylus megistus from the same region (Vallejo et al 2002(Vallejo et al , 2015. This may reflect a process of speciation within T. rangeli, as advanced by some authors (Macedo et al 1993, closely associated to the vector lineages (Machado et al 2001;Vallejo et al 2002Vallejo et al , 2015Urrea et al 2005Urrea et al , 2011Maia-da-Silva et al 2007;Salazar-Antón et al 2009;Barreto-Santana et al 2015;Sincero et al 2015). At present, with so much available knowledge on T. rangeli genetic diversity, we question whether the KP1(-) group from southern Brazil could be a subspecies.…”

Section: Discussionmentioning

confidence: 52%

“…The stocks of T. rangeli were subsequently denoted as KP1(+) and KP1(-) according to the presence or absence of this minicircle class (Vallejo et al 2002). Studies on the PCR products from the spliced-leader intergenic region (SL-IR) confirmed the dichotomy in T. rangeli and expanded the knowledge on their geographic distribution in the Latin America (Grisard et al 1999a;Vallejo et al 2003Vallejo et al , 2015Urrea et al 2005Urrea et al , 2011Salazar-Antón et al 2009). Several authors reported associations between the two main T. rangeli groups and their regional vectors, besides their development pattern within these insects (Machado et al 2001;Vallejo et al 2003Vallejo et al , 2015Urrea et al 2005Urrea et al , 2011Salazár-Antón et al 2009).…”

Section: Introductionmentioning

confidence: 99%

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Peculiarities of Trypanosoma rangeli KP1(-) Strains Isolated from the Wild Rodent Phyllomys dasythrix (Santa Catarina, Brazil): Comparisons with T. rangeli KP1(+) strains and Trypanosoma lewisi (Kinetoplastea, Trypanosomatidae)

Sousa

Santos

Carvalhal

et al. 2017

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Two KP1(-) strains of Trypanosoma rangeli (SC-58, SC-61) isolated from the wild rodent Phyllomys dasythrix from Santa Catarina (Brazil) were compared with some KP1(+) reference stocks from different Latin America countries, and also with Trypanosoma lewisi. The strains were analyzed by some morphological and biological features, and by biochemical and molecular techniques. The mean total length (TL) of the bloodstream trypomastigotes of T. rangeli varied between 31.3-33.0 µm, and those of T. lewisi (adult forms) was 28.2 µm, values within the variation known for each species. In T. rangeli KP1(+) and T. lewisi, the nucleus was located in the anterior portion of the body, with nuclear indexes (NI) ≥ 1.2, as typically described for both species. Differently, most trypomastigotes of the KP1(-) stocks presented NI ≤ 1.0. Another striking feature of the KP1(-) strains was their very fastidiously growth in axenic cultures when compared with the KP1(+) stocks and T. lewisi. Three isoenzyme loci (MDH, IDH and PGM) clearly distinguished T. rangeli and T. lewisi, and the distinction between the KP1(+) and KP1(-) strains was possible at MDH, PGM and GPI loci. All T. rangeli strains presented the typical 760 bp amplicon derived from their KP2 minicircles. However, the KP3 products of the KP1(+) strains were a single large band (~330bp), whereas those of the KP1(-) had two distinct bands (350 and 300 bp). T. lewisi presented 700 and 400 bp amplicons, as previously reported. The peculiarities of T. rangeli isolates from P. dasythrix corroborate a possible speciation process within this taxon.

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Section: Biochemical and Molecular Analysessupporting

confidence: 89%

Section: Discussionsupporting

confidence: 61%

Section: Discussionmentioning

confidence: 52%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Peculiarities of Trypanosoma rangeli KP1(-) Strains Isolated from the Wild Rodent Phyllomys dasythrix (Santa Catarina, Brazil): Comparisons with T. rangeli KP1(+) strains and Trypanosoma lewisi (Kinetoplastea, Trypanosomatidae)

Sousa

Santos

Carvalhal

et al. 2017

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“…It is recognized that the species of triatominae belonging to the genus Rhodnius are susceptible to infection by Trypanosoma rangeli, and the occurrence has already been recorded in the following species: Rhodnius domesticus, Rhodnius nasutus, Rhodnius neglectus 4 8 .…”

mentioning

confidence: 99%