Trypanosoma rangeli: Differential expression of cell surface polypeptides and ecto-phosphatase activity in short and long epimastigote forms

Gomes, Sérgio Bittencourt Varella; Souza, André Luíz Fonseca de; Silva, Bianca A.; Kiffer-Moreira, Tina; Santos-Mallet, Jacenir Reis dos; Santos, André Luis Souza dos; Meyer‐Fernandes, José Roberto

doi:10.1016/j.exppara.2005.11.015

Cited by 44 publications

(28 citation statements)

References 45 publications

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“…Short epimastigotes were more resistant to lysis and stimulated greater superoxide and prophenoloxidase responses than long form epimastigotes of T. rangeli (32,33). Moreover, the ecto-phosphatase activity of short forms was more sensitive than that found in the long form (34,35). Finally, long but not short epimastigotes adhered to the gland cells and the strength of interaction correlated with the enzyme activity levels (36).…”

Section: Discussionmentioning

confidence: 78%

Susceptibilidad de diferentes especies de Rhodnius (Hemiptera, Reduviidae, Triatominae) a una cepa brasileña de Trypanosoma rangeli (SC58/KP1-)

et al. 2014

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Introduction: Specific host-parasite associations have been detected experimentally and suggest that triatomines of the genus Rhodnius act as biological filters in the transmission of Trypanosoma rangeli. Objective: To analyze the susceptibility of four Rhodnius species (Rhodnius robustus, Rhodnius neglectus, Rhodnius nasutus and Rhodnius pictipes) to a Brazilian strain of T. rangeli (SC-58/KP1-). Materials and methods:We selected thirty nymphs of each species, which were fed on blood infected with T. rangeli. Periodically, samples of feces and hemolymph were analyzed. Triatomines with T. rangeli in their hemolymph were fed on mice to check for transmission by bites. Later, the triatomines were dissected to confirm salivary gland infection. Results: Specimens of R. pictipes showed higher rates of intestinal infection compared to the other three species. Epimastigotes and trypomastigotes were detected in hemolymph of four species; however, parasitism was lower in the species of the R. robustus lineage. Rhodnius robustus and R. neglectus specimens did not transmit T. rangeli by bite; after dissection, their glands were not infected. Only one specimen of R. nasutus and two of R. pictipes transmitted the parasite by bite. The rate of salivary gland infection was 16% for R. pictipes and 4% for R. nasutus. Conclusions: Both infectivity (intestinal, hemolymphatic and glandular) and transmission of T. rangeli (SC58/KP1-) were greater and more efficient in R. pictipes. These results reinforce the hypothesis that these triatomines may act as biological filters in the transmission of T. rangeli.

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Section: Discussionmentioning

confidence: 78%

Susceptibilidad de diferentes especies de Rhodnius (Hemiptera, Reduviidae, Triatominae) a una cepa brasileña de Trypanosoma rangeli (SC58/KP1-)

et al. 2014

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“…Only a few soluble enzymes have been detected in the acidocalcisomal matrix, including an exopolyphosphatase (PPX) in Leishmania major and Trypanosoma cruzi (Fang et al 2007b), a soluble inorganic pyrophosphatase in Trypanosoma brucei (Lemercier et al 2004), and a metacaspase in Leishmania donovani (Lee et al 2007). Acid phosphatase activity has also been detected using cytochemical methods (Gomes et al 2006).…”

Section: Structural Characteristics and Composition Of Acidocalcisomesmentioning

confidence: 99%

Evolution of acidocalcisomes and their role in polyphosphate storage and osmoregulation in eukaryotic microbes

Docampo

Ulrich

Moreno

2010

Phil. Trans. R. Soc. B

102

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Acidocalcisomes are acidic electron-dense organelles, rich in polyphosphate (poly P) complexed with calcium and other cations. While its matrix contains enzymes related to poly P metabolism, the membrane of the acidocalcisomes has a number of pumps (Ca 2+ -ATPase, V-H + -ATPase, H + -PPase), exchangers (Na + /H + , Ca 2+ /H + ), and at least one channel (aquaporin). Acidocalcisomes are present in both prokaryotes and eukaryotes and are an important storage of cations and phosphorus. They also play an important role in osmoregulation and interact with the contractile vacuole complex in a number of eukaryotic microbes. Acidocalcisomes resemble lysosome-related organelles (LRO) from mammalian cells in many of their properties. They share similar morphological characteristics, acidic properties, phosphorus contents and a system for targeting of their membrane proteins through adaptor complex-3 (AP-3). Storage of phosphate and cations may represent the ancestral physiological function of acidocalcisomes, with cation and pH homeostasis and osmoregulatory functions derived following the divergence of prokaryotes and eukaryotes.

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“…The first study on PTPs in T. rangeli showed that there are differences in tyrosine phosphatase activity between the long and short epimastigote forms (Gomes et al 2006). The importance of this group of proteins for the T. rangeli life cycle was reaffirmed by the finding that inhibition of surface ectophosphatases impaired parasite proliferation (Fonsecade-Souza et al 2009).…”

mentioning

confidence: 79%

Trypanosoma rangeli protein tyrosine phosphatase is associated with the parasite's flagellum

Prestes¹,

Bayer-Santos

Stoco

et al. 2012

Mem. Inst. Oswaldo Cruz

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Trypanosoma rangeli: Differential expression of cell surface polypeptides and ecto-phosphatase activity in short and long epimastigote forms

Cited by 44 publications

References 45 publications

Susceptibilidad de diferentes especies de Rhodnius (Hemiptera, Reduviidae, Triatominae) a una cepa brasileña de Trypanosoma rangeli (SC58/KP1-)

Susceptibilidad de diferentes especies de Rhodnius (Hemiptera, Reduviidae, Triatominae) a una cepa brasileña de Trypanosoma rangeli (SC58/KP1-)

Evolution of acidocalcisomes and their role in polyphosphate storage and osmoregulation in eukaryotic microbes

Trypanosoma rangeli protein tyrosine phosphatase is associated with the parasite's flagellum

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