Trypanosoma cruzi infectivity assessment in “in vitro” culture systems by automated cell counting

Caspase-8 activity is part of the BeWo trophoblast cell defense mechanisms against Trypanosoma cruzi infection

“…The cells were analyzed at 48 hours post-infection. For T. cruzi amastigote identification, infected cells were processed for DAPI (4, 6-diamidino-2-phenylindole, Molecular Probes; 1ug DAPI/ml) nuclear staining and amastigotes were recognized by their nuclear size and presence of kinetoplast ( Fig 4C) and quantified using MATLAB® software (Liempi et al 2015).…”