Trypanosoma cruzi infection induces morphological reorganization of the myocardium parenchyma and stroma, and modifies the mechanical properties of atrial and ventricular cardiomyocytes in rats

Curcumin Enhances the Anti-Trypanosoma cruzi Activity of Benznidazole-Based Chemotherapy in Acute Experimental Chagas Disease

“…Histological sections (3 m thick) were obtained with a rotary microtome and glass knives. The sections were evaluated in semiseries (using one out of every 20 sections) and were stained with hematoxylin and eosin (32). For each histological section, six microscope images were captured at ϫ400 magnification (Axioscope A1; Zeiss, Germany).…”

“…The intensity of myocardial inflammation was assessed by the stereological method (32). For this, a quadratic test system with a standardized test area (A t ) (A t ϭ 0.0423 mm 2 ) was applied to the histological images.…”

Euterpe edulis extracts positively modulates the redox status and expression of inflammatory mediators

“…For stereology analysis, a 300-point test system was used in a standard test area (At) of 73 × 103 µm 2 . The volume densities (vv) were estimated by counting points using the following formula: vv = PP[structure]/PT; where PP is the number of points located along the structure in question and PT are the total test points Novaes et al, 2013). The volume of 50 hepatocyte nuclei for each animal was determined according to Novaes et al (2013).…”

“…The volume densities (vv) were estimated by counting points using the following formula: vv = PP[structure]/PT; where PP is the number of points located along the structure in question and PT are the total test points Novaes et al, 2013). The volume of 50 hepatocyte nuclei for each animal was determined according to Novaes et al (2013). All morphological analyses were performed using the Pro-Plus image analysis software 4.5 (Media Cybernetics, Silver Spring, MD, USA).…”

Rescue Therapy with Nifurtimox and Dipyridamole for Severe Acute Chagas Myocarditis with Congestive Heart Failure in NMRI Albino Mice

“…Improvement of intraventricular conduction may reflect an inflammation and blood flow restoration in a reversible morphofunctional substrate, while irreversible AV conduction disorders, including arrhythmias such as atrial fibrillation, which seems to be a reflection of an irreversible damage, product of intense inflammation, remodeling and fibrosis of the atria, usually observed in ChD. 29 Likewise, the R wave amplitude was unchanged, which might be due to irreversible damage to the myocardial structure or it could reflect a net pharmacological effect due to dipyridamole-induced negative inotropic effect mediated by adenosine acting on A1 receptors. 9 Mast cell density in heart tissue histological sections was higher in the CC group when compared to the NifDip group.…”