Trypanosoma cruzi: genetic group with peculiar biochemical and biological behavior

Gomes, Mônica Lúcia; Toledo, Max Jean de Ornelas; Nakamura, Celso Vataru; Bittencourt, Nilza de Lucas Rodrigues; Chiari, Égler; Araújo, Silvana Márques de

doi:10.1590/s0074-02762003000500011

Cited by 14 publications

(18 citation statements)

References 27 publications

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“…We found a possible correlation between genetic characteristics (microsatellite genotype 2) and susceptibility to the drug benznidazole, described previously only in South American strains (Murta et al 1998, Gomes et al 2003, Toledo et al 2003.…”

Section: Discussionsupporting

confidence: 67%

“…The association of genetic markers with drug susceptibility in T. cruzi has been studied in some South American strains (Murta et al 1998, Gomes et al 2003. However, only a few studies have focused on the susceptibility of Mexican T. cruzi strains to nifurtimox or benznidazole, and the Mexican T. cruzi strains used in these studies have not been well characterized genetically (Leó n-Pérez et al 2007).…”

Section: Introductionmentioning

confidence: 99%

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Microsatellite and Mini-Exon Analysis of Mexican Human DTU ITrypanosoma cruziStrains and Their Susceptibility to Nifurtimox and Benznidazole

Martı́nez

Nogueda

Martínez‐Hernández

et al. 2013

Vector-Borne and Zoonotic Diseases

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Chagas disease is caused by the protozoan parasite Trypanosoma cruzi, and it affects as many as 10 million people in North and South America, where it represents a major public health problem. T. cruzi is a parasite with high genetic diversity, and it has been grouped into 6 discrete typing units (DTUs), designated as T. cruzi I (TcI) to T. cruzi VI (TcVI). Mexican isolates from humans and from vector insects have been primarily found to be TcI, and these isolates are likely to be the strains that cause the clinical manifestations observed in Mexico. However, genetic characterization and drug susceptibility assays are limited in Mexican TcI strains. In this work, 24 Mexican T. cruzi strains, obtained primarily from humans, were studied with 7 locus microsatellites and mini-exon gene by PCR. Also, drug susceptibility was evaluated by growth and mobility assays. All of the human strains belonged to TcI, and they could be further grouped through microsatellite analysis into 2 subgroups (microsatellite genotypes 1 and 2), which were not related to the host clinical status or biological origin of the strain. Two strains, both from wild mammals, belonged to the TcII-TcVI groups; these strains and the CL Brener strain constituted microsatellite genotype 3. The number of alleles in each locus was lower than reported for South American strains, and a departure from the Hardy-Weinberg equilibrium was observed. The susceptibility of these strains to nifurtimox and benznidazole was heterogeneous. T. cruzi strains characterized as microsatellite genotypes 2 and 3 were significantly more susceptible to benznidazole than strains of microsatellite genotype 1. Only 1 Mexican strain resistant to both drugs was found in this study.

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Section: Discussionsupporting

confidence: 67%

Section: Introductionmentioning

confidence: 99%

Microsatellite and Mini-Exon Analysis of Mexican Human DTU ITrypanosoma cruziStrains and Their Susceptibility to Nifurtimox and Benznidazole

Martı́nez

Nogueda

Martínez‐Hernández

et al. 2013

Vector-Borne and Zoonotic Diseases

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“…T. cruzi causes Chagas' disease, which is characterized by a variable clinical course that ranges from the absence of symptoms to severe disease with cardiac involvement and/or digestive tract damage. This variability has been attributed both to differences in the host response and to the genomic heterogeneity of the parasite (2,7,12,23,25,27,31,33,34,42,44).…”

mentioning

confidence: 99%

“…Many research groups have tried to correlate the genetic variability of the parasite with the clinical manifestation of the disease, but they were unsuccessful (6,16,22,23,26,49). In light of the histotropic-clonal model proposed for Chagas' disease in the beginning of the 1980s, at least two explanations can be devised.…”

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confidence: 99%

Probing Population Dynamics of Trypanosoma cruzi during Progression of the Chronic Phase in Chagasic Patients

et al. 2009

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Our research aimed to characterize the genetic profiles of 102 Trypanosoma cruzi isolates recently obtained from 44 chronic chagasic patients from different regions of the states of Minas Gerais and Goiás in Brazil. At least two isolates were obtained from each patient at different times in order to study the parasite population dynamics during disease progression in the chronic phase. The isolates were characterized molecularly by genotyping the 3 region of the 24S␣ rRNA, the mitochondrial cytochrome oxidase subunit 2 (COII) gene, and the intergenic region of the spliced leader intergenic region (SL-IR) gene. Seventy-seven isolates were analyzed for nine microsatellite loci. The data presented here show a strong correlation between the T. cruzi lineage II (T. cruzi II) and human infection in these regions of Brazil. Interestingly, isolates from two patients were initially characterized (by rRNA genotyping) as T. cruzi I and hybrid strains, but subsequent analyses of the COII and SL-IR genes confirmed that those isolates belonged to T. cruzi III and a hybrid group, respectively. Our results confirm the risk of misclassifying T. cruzi isolates on the basis of analysis of a single molecular marker. The microsatellite profiles showed that different isolates obtained from the same patient were genetically identical and monoclonal. Exceptions were observed for T. cruzi isolates from two patients who presented differences for the SCLE11 locus and also from two other patients who showed amplification of three peaks for a microsatellite locus (TcAAAT6), implying that they were multiclonal. On the basis of the findings of the studies described here, we were not able to establish a correlation between the clinical forms of Chagas' disease and the genetic profiles of the T. cruzi isolates.

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“…cruzi strains present a large biochemical and genetic variability (Gomes et al 2003, Macedo et al 2004), leading to astounding differences in terms of morphology, tissue tropism, virulence and drug susceptibility (de Diego et al 1998, Andrade andMagalhães 1997). Such different characteristics stimulated a search for new molecular markers that allow the correlation between protozoan genotype and clinical manifestations, leading to a more complete diagnosis and better treatment protocols.…”

mentioning

confidence: 99%

Expanding the knowledge of the chemical structure of glycoconjugates from Trypanosoma cruzi TcI genotype. Contribution to taxonomic studies

Fonseca

Garcez

Penha

et al. 2016

An. Acad. Bras. Ciênc.

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One of the main obstacles to the treatment of Chagas disease is the genetic and phenotypical variance displayed by T. cruzi strains, resulting in differences in morphology, virulence, pathogenicity and drug susceptibility. To better understand the role of glycoconjungates in Chagas disease, we performed the molecular characterization of the O-linked chains from mucins and glycoinositolphospholipids (GIPLs) of the Silvio X10 clone 1 strain. We demonstrated the presence of a β-galactofuranose (β-Galf) unity linked to the O-4 position of the α-N-acetylglucosamine (α-GlcNAc)O-4 in Tc-mucins. GIPLs analysis showed that the lipidic portion is exclusively composed of ceramide and the PI-oligossacharidic portion contains the Man4(AEP)GlcN-Ins-PO 4 core, substituted by ethanolamine-phosphate (EtNP) on the third distal mannose from inositol, which may or may not have a terminal β Galf unity. These results confirm the classification of the Silvio X10/1 strain in group T. cruzi I. Again, it is noted that the study of T. cruzi surface glycoconjugates confirm the molecular results and the hypothesis that surface glycoconjugates may be interesting biomarker for the differentiation of trypanosomatid strains.

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Trypanosoma cruzi: genetic group with peculiar biochemical and biological behavior

Abstract: Thirty-two

Cited by 14 publications

References 27 publications

Microsatellite and Mini-Exon Analysis of Mexican Human DTU ITrypanosoma cruziStrains and Their Susceptibility to Nifurtimox and Benznidazole

Microsatellite and Mini-Exon Analysis of Mexican Human DTU ITrypanosoma cruziStrains and Their Susceptibility to Nifurtimox and Benznidazole

Probing Population Dynamics of Trypanosoma cruzi during Progression of the Chronic Phase in Chagasic Patients

Expanding the knowledge of the chemical structure of glycoconjugates from Trypanosoma cruzi TcI genotype. Contribution to taxonomic studies

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