Trypanosoma cruzi: Effect of the infection on the 20S proteasome in non-immune cells

Faria, Líliam Oliveira; Lima, Beatriz Dolabela de; Sá, Cezar Martins de

doi:10.1016/j.exppara.2008.08.003

Cited by 6 publications

(8 citation statements)

References 35 publications

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“…Taken together, the results of mRNA and protein expression indicate that the biogenesis of the constitutive proteasome is not affected by the parasite T. cruzi . These data are consistent with our previous results [40].…”

Section: Resultssupporting

confidence: 94%

“…Trypomastigotes were incubated with HeLa cells at 37°C for 24 h or longer at a parasite-host cell ratio of 40:1. At this ratio, 80–100% of the host cells presented amastigotes inside their cytoplasm after 24 h of parasite inoculation [40].…”

Section: Methodsmentioning

confidence: 95%

“…[40]. Primers used in real-time RT-qPCR for amplification of GAPDH , HPRT1 and for the small subunit of the MHC class I molecule called β2-microglobulin (β2M) are described in Ref.…”

Section: Methodsmentioning

confidence: 99%

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Trypanosoma cruzi Infection Down-Modulates the Immunoproteasome Biosynthesis and the MHC Class I Cell Surface Expression in HeLa Cells

et al. 2014

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Generally, Trypanosoma cruzi infection in human is persistent and tends to chronicity, suggesting that the parasite evade the immune surveillance by down regulating the intracellular antigen processing routes. Within the MHC class I pathway, the majority of antigenic peptides are generated by the proteasome. However, upon IFN-γ stimulation, the catalytic constitutive subunits of the proteasome are replaced by the subunits β1i/LMP2, β2i/MECL-1 and β5i/LMP7 to form the immunoproteasome. In this scenario, we analyzed whether the expression and activity of the constitutive and the immunoproteasome as well as the expression of other components of the MHC class I pathway are altered during the infection of HeLa cells with T. cruzi. By RT-PCR and two-dimensional gel electrophoresis analysis, we showed that the expression and composition of the constitutive proteasome is not affected by the parasite. In contrast, the biosynthesis of the β1i, β2i, β5i immunosubunits, PA28β, TAP1 and the MHC class I molecule as well as the proteasomal proteolytic activities were down-regulated in infected-IFN-γ-treated cell cultures. Taken together, our results provide evidence that the protozoan T. cruzi specifically modulates its infection through an unknown posttranscriptional mechanism that inhibits the expression of the MHC class I pathway components.

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Section: Resultssupporting

confidence: 94%

Section: Methodsmentioning

confidence: 95%

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Trypanosoma cruzi Infection Down-Modulates the Immunoproteasome Biosynthesis and the MHC Class I Cell Surface Expression in HeLa Cells

et al. 2014

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“…There is one result regarding lymphocyte infection in studies using PBMCs [28]. In a similar fashion to primary human monocytes/macrophages, HeLa cells and derivatives (HEp-2) were highly susceptible to T. cruzi infection (80% of infected cells at 24 h) regardless of the parasite strain or MOI used [8, 19, 26]. Similar percentages of infected cells (approximately 80%) were observed when HUVEC or HUSMC (human umbilical smooth muscle cells) cells were used.…”

Section: Resultsmentioning

confidence: 99%

Mammalian cellular culture models ofTrypanosoma cruziinfection: a review of the published literature

et al. 2014

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Cellular culture infection with Trypanosoma cruzi is a tool used to dissect the biological mechanisms behind Chagas disease as well as to screen potential trypanocidal compounds. Data on these models are highly heterogeneous, which represents a challenge when attempting to compare different studies. The purpose of this review is to provide an overview of the cell culture infectivity assays performed to date. Scientific journal databases were searched for articles in which cultured cells were infected with any Trypanosoma cruzi strain or isolate regardless of the study’s goal. From these articles the cell type, parasite genotype, culture conditions and infectivity results were extracted. This review represents an initial step toward the unification of infectivity model data. Important differences were detected when comparing the pathophysiology of Chagas disease with the experimental conditions used in the analyzed studies. While Trypanosoma cruzi preferentially infects stromal cells in vivo, most of the assays employ epithelial cell lines. Furthermore, the most commonly used parasite strain (Tulahuen-TcVI) is associated with chagasic cardiomyopathy only in the Southern Cone of South America. Suggestions to overcome these discrepancies include the use of stromal cell lines and parasite genotypes associated with the known characteristics of the natural history of Chagas disease.

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“…This could be the explanation for the chronic phase of the Chagas' disease in the same way that certain viruses have developed strategies to evade class Irestricted antigen processing and presentation [126]. When Faria et al [127] compared T. cruzi-infected HeLa cells with non-infected cells, no differences between the composition of 20S proteasome and expression of its subunits were observed. This included the interferon-inducible subunits and failed to induce the formation of immunoproteasome.…”

Section: Proteasomementioning

confidence: 99%

Biological Roles of Peptidases in Trypanosomatids~!2009-11-26~!2010-02-15~!2010-03-18~!

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Branquinha²,

d’Avila-Levy³

et al. 2010

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Abstract:In this review, we report the recent developments in the characterization of peptidases and their possible biological functions in the Trypanosomatidae family. The focus will be on peptidases from Trypanosoma cruzi, Leishmania spp., African trypanosomes and plant and insect trypanosomatids. There are numerous events in parasite development where the involvement of peptidases has been established, and they will be approached in the present review. Also in this review we will discuss the central roles have been proposed for peptidases in diverse processes such as virulence, host cell interaction and invasion, catabolism of host proteins, differentiation, cell cycle progression and both stimulation and evasion of host immune responses.

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Trypanosoma cruzi: Effect of the infection on the 20S proteasome in non-immune cells

Cited by 6 publications

References 35 publications

Trypanosoma cruzi Infection Down-Modulates the Immunoproteasome Biosynthesis and the MHC Class I Cell Surface Expression in HeLa Cells

Trypanosoma cruzi Infection Down-Modulates the Immunoproteasome Biosynthesis and the MHC Class I Cell Surface Expression in HeLa Cells

Mammalian cellular culture models ofTrypanosoma cruziinfection: a review of the published literature

Biological Roles of Peptidases in Trypanosomatids~!2009-11-26~!2010-02-15~!2010-03-18~!

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