Trypanosoma cruzi: Conformational Preferences of Antigenic Peptides Bearing the Immunodominant Epitope of the B13 Antigen

Duranti, M A; Franzoni, L.; Sartor, Giorgio; Benedetti, Arianna; Iwai, Leo Kei; Gruber, Arthur; Zingales, Bianca; Guzmán, Fanny; Kalil, Jorge; Spisni, Alberto; Cunha-Neto, Edécio

doi:10.1006/expr.1999.4428

Cited by 10 publications

(11 citation statements)

References 24 publications

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“…Serological tests have high sensitivity but lack specificity due to the antigenic cross-reactivity with parasite. Several studies have been developed to find a more specific protein [4,5] and different approaches have also been proposed for diagnosing the Chagas' disease like impedimetric evaluation [6], polymerase chain reaction [7,8], or amperometric immunosensor [9].…”

Section: Introductionmentioning

confidence: 99%

Investigation of the interaction between Tc85-11 protein and antibody anti-T. cruzi by AFM and amperometric measurements

Ferreira

Colli²,

Alves³

et al. 2006

Electrochimica Acta

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Section: Introductionmentioning

confidence: 99%

Investigation of the interaction between Tc85-11 protein and antibody anti-T. cruzi by AFM and amperometric measurements

Ferreira

Colli²,

Alves³

et al. 2006

Electrochimica Acta

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“…This has also been reported for a few other recombinant T. cruzi antigens, such as antigens JL5, JL9, and the C-terminal region of T. cruzi HSP70 [27]. B13 and JL5 have been described as cross-reactive with cardiac myosin [28–30] and cardiovascular beta-adrenergic and cholinergic receptors respectively [31]. It is possible that the higher levels displayed in sera from CCC patients of these two potentially pathogenic cross-reactive antibody systems are biologically relevant in the generation of pathological autoimmunity.…”

Section: Discussionmentioning

confidence: 52%

Evidence for T Cell Help in the IgG Response against Tandemly RepetitiveTrypanosoma cruziB13 Protein in Chronic Chagas Disease Patients

Duranti

Camargo

Victora

et al. 2012

Journal of Parasitology Research

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The tandemly repetitive Trypanosoma cruzi B13 protein is an immunodominant antigen among Chagas disease patients. Such repetitive domains may behave as T-independent antigens. However, T cells can recognize B13 epitopes in an HLA class II-restricted fashion and could potentially provide cognate T cell help and boost antibody titers. We assessed whether the presence of HLA class II molecules able to present B13 epitopes to T cells could affect anti-B13 IgG levels in a cognate fashion, in both major clinical forms of chronic Chagas disease. We found no difference between anti-B13 IgG antibody levels between patients carrying HLA class II molecules associated to T cell responses or other alleles. The predominant anti-B13 IgG subclass was IgG1, with negligible IgG2, suggesting a T-dependent, noncognate help for antibody production. In addition, the finding of increased anti-B13 IgG levels in sera from CCC patients indicates that clinical presentation is associated with increased anti-B13 antibody levels.

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“…Previous work indicated that the sequence FGQAAAGDK (peptide S4) carries the immunodominant epitope of B13 antigen and that the sequence AAAGDK should be the core of the epitope [6,8]. B13.1–LamB and B13.2–LamB constructs display different antigenic activities, confirming that the residues flanking the core AAAGDK modulate the interaction of the human antibodies with B13 antigen.…”

Section: Resultsmentioning

confidence: 90%

“…Peptides encompassing linear epitopes of B13 antigen (Table 1) were synthesized by solid‐phase Merrifield “teabag” technology from t‐Boc derivatives, HPLC purified, and checked by mass spectrometry [6,8].…”

Section: Methodsmentioning

confidence: 99%

“…This analysis indicated that the peptide FGQAAAGDK contained the immunodominant epitope of B13 and that the 7‐mer sequence QAAAGDK should be the core of that epitope [6,7]. Conformational preferences of synthetic peptides bearing the immunodominant epitope of the B13 antigen have been investigated by our group by circular dichroism and 1 H nuclear magnetic resonance [8]. The data suggested a correlation between the propensity of the peptides to adopt a helical configuration centered in the AAAGDK sequence and their antigenicity.…”

Section: Introductionmentioning

confidence: 92%

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Epitope mapping of a single repetitive unit of the B13Trypanosoma cruziantigen as fusions toEscherichia coliLamB protein

Pereira¹,

Silveira²,

Duranti³

et al. 2004

FEMS Microbiology Letters

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B13, one of the immunodominant antigens of Trypanosoma cruzi, is composed of repeats of a 12-amino-acid motif. Using synthetic peptides, the sequence FGQAAAGDK was previously shown to contain the B13 immunodominant epitope recognized by chagasic patients sera. To investigate the effects of neighboring sequences in the immunodominance, we tested serum recognition of two B13 sequences fused to LamB. GDKPSPFGQAAA-LamB and FGQAAAGDKPSP-LamB were recognized, respectively, by 15% and 80% of 80 sera reactive to B13 antigen. Recognition of FGQAAAGDKPSP-LamB was inhibited by AAAGDK-containing synthetic peptides. FGQAAAGDKPSP-LamB competed with a B13 recombinant protein containing 16.6 repeats for binding to chagasic antibodies. These results strengthen previous conclusions on the immunodominant epitope of B13 and provide a comparison of two methods for epitope mapping.

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Trypanosoma cruzi: Conformational Preferences of Antigenic Peptides Bearing the Immunodominant Epitope of the B13 Antigen

Cited by 10 publications

References 24 publications

Investigation of the interaction between Tc85-11 protein and antibody anti-T. cruzi by AFM and amperometric measurements

Investigation of the interaction between Tc85-11 protein and antibody anti-T. cruzi by AFM and amperometric measurements

Evidence for T Cell Help in the IgG Response against Tandemly RepetitiveTrypanosoma cruziB13 Protein in Chronic Chagas Disease Patients

Epitope mapping of a single repetitive unit of the B13Trypanosoma cruziantigen as fusions toEscherichia coliLamB protein

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