Trypanosoma Cruzi: a Carbohydrate Epitope Defined by a Monoclonal Antibody as a Possible Marker of the Acute Phase of Human Chagas' Disease

Trypanosoma cruzi high infectivity in vitro is related to cardiac lesions during long-term infection in Beagledogs

“…Previous studies with acutely infected chagasic patients had shown the presence of IgA, IgM, and IgG antibodies. Also it has been shown that IgM and IgG are detected in the beginning of the chronic phase, but only IgG is present along the chronic phase (Magnani et al 1973, Camargo & Amato Neto 1974, Scott & GossSampson 1984, Ouaissi et al 1991, Grauert et al 1993, Medrano-Mercado et al 1996. Concordant results were observed in experimentally infected dogs with T. cruzi (Andrade et al 1981, Bambirra et al 1984, Barr et al 1991, Guedes et al 2002.…”

“…Besides IgA antibody levels decreased (15 weeks after infection) earlier than the IgM isotype (20 weeks after infection). Since IgM antibodies have been detected for longer periods in patients (Ouaissi et al 1991) and in the canine model during the initial chronic phase of the infection (Lana et al 1992), we suggest here that the association of IgA and IgM antibody levels are certainly useful specific markers for the acute chagasic infection. As expected IgG isotype levels were higher during the chronic phase.…”

Evaluation of the western blot in the confirmatory serologic diagnosis of Chagas' disease.

“…17 The antigen was prepared by previously published methods, with some modifications. 8,12,18,19 The cells were pelleted by centrifugation at 3,200 ϫ g for 10 min at 4ЊC, and washed three times with 150 mM phosphate-buffered saline (PBS), pH 7.2, by centrifugation at 3,200 ϫ g for 10 min at 4ЊC. The sediment was resuspended in 20 mM Tris-HCl, pH 7.5, containing 1 mM phenylmethylsulfonyl fluoride, 1 mM o-phenanthroline, and 10 g/ml of L-1-tosylamide 2,2-phenylethylchloromethylketone.…”

A century of research: what have we learned about the interaction of Trypanosoma cruzi with host cells?

“…The parasite infective forms considered herein are metacyclic (MT) and tissue-culture derived trypomastigotes (TCTs), as well as extracellular amastigotes (Fig. 2) 19�4, 19�5), de Lederkremer et al (1980), Lima and Kierszenbaum (1982), Zingales et al (1982), de Lederkremer and Colli (1995); d: Brener (19�9), Silva and Nussenzweig (195�), Howells and Chiari (19�5), Brener (19��), Melo and Brener (19�8), Bice and Zeledon (19�0); e: Miles (19�4),Brener (19��), �lcantara and Brener (19�8), Melo and Brener (19�8), Souto et al (199�) Ouaissi et al (1991), Travassos and �lmeida (199�), Previato et al (1994), Souto-Padrón et al (1994), Parodi et al (1995), Kahn et al (199�), Silva et al (1998, 200�); g: Nogueira and Cohn (19��), Henriquez et al (1981), Lima and Kierszenbaum (1982), Piras et al (1982Piras et al ( , 198�, 1985, Nogueira (198�), Villalta and Kierszenbaum (198�, 1984), Ouaissi et al (1985), Snary (1985), Schenkman et al (1991a), Schenkman and Mortara (1992); h: Piras et al (1985), de Titto and �raujo (198�), Mbawa et al (1991), McKerrow (1991, Schenkman et al (1991b, Eakin et al (1992), Meirelles et al (1992), Chaves et al (199�),Colli (199�), Cross and Takle (199�), Burleigh et al (199�), S...…”