Trypanosoma congolense: Specific transformation in vitro of leukocytes from infected or immunized cattle

Emery, D.L.; Wells, P.W.; Tenywa, Tom

doi:10.1016/0014-4894(80)90039-9

Cited by 18 publications

(9 citation statements)

References 16 publications

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“…Earlier parasite-specific antibody levels in trypanotolerant cattle have been reported by some groups (Akol et al 1986, Pinder et al 1988), but have not been detected by others . In contrast, humoral immune responses are known to play an important role in limiting homologous rechallenge infections in cattle, goats and rabbits, with antibodies specific for both the mVATs and for bloodstream (bsf) VATS involved in protection (Emery et al 1980, Wellde et al 1981, Akol & Murray 1983, Nantulya et al 1984. The results reported here show that the N'Damas had a superior anti-mVATspecific response prior to the rechallenge infection compared to the Borans.…”

Section: Discussionmentioning

confidence: 60%

Susceptibility of N'Dama and Boran cattle to tsetse‐transmitted primary and rechallenge infections with a homologous serodeme of Trypanosoma congolense

Paling

Moloo²,

Scott³

et al. 1991

Parasite Immunology

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Eight trypanotolerant N'Dama cattle controlled an infection of Trypanosoma congolense ILNat 3.1 transmitted by Glossina morsitans centralis, more efficiently than a group of similarly infected trypanosusceptible Boran cattle. All eight N'Damas maintained their PCV above 15% throughout the primary infection whereas the PCV of six of the eight Borans dropped below 15%; these latter animals were treated with diminazene aceturate to prevent possible death. Lymphocyte, neutrophil and platelet counts also decreased in the Boran during the primary infection. In contrast, a lymphocytosis was observed in the N'Dama; and although the neutrophil and platelet counts decreased, the drop was less severe than in the Boran. Two years after the primary infection and immediately prior to a homologous rechallenge infection, all eight N'Damas had neutralizing anti-metacyclic trypanosome variant-specific antibodies present in their sera compared to five of the eight Borans. Following the homologous rechallenge infection the eight N'Damas became parasitaemic but there were no alterations in their erythrocyte or leukocyte counts. The Borans became highly parasitaemic and developed severe, chronic anaemia and leukopaenia. Thus, the trypanotolerant N'Damas controlled a primary infection of T. congolense more efficiently than trypanosusceptible Boran cattle and eliminated a homologous rechallenge infection without the pathology associated with the disease.

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Section: Discussionmentioning

confidence: 60%

Susceptibility of N'Dama and Boran cattle to tsetse‐transmitted primary and rechallenge infections with a homologous serodeme of Trypanosoma congolense

Paling

Moloo²,

Scott³

et al. 1991

Parasite Immunology

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“…PBL from these animals failed to respond to stimulation with either live or soluble antigens or its purified variable surface glycoprotein until the ponies were treated [21,22]. Similarly, PBL from cattle infected with T. congolense failed to exhibit specific blastogenesis in vitro in response to stimulation with ultrasonicated T. congolense antigen, while cells from infected but treated animals responded normally [27]. Moreover, in cattle cyclically infected with T. congolense, PBL collected between days 3 and 7 p.i.…”

Section: Discussionmentioning

confidence: 99%

Proliferative Responses of Peripheral Blood Leucocytes of Sheep Infected with Trypanosoma evansi

1998

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The effects of Trypanosoma evansi on the proliferative responses of ovine peripheral blood leucocytes (PBL) were examined in in vitro cell culture systems. Sheep were vaccinated against pneumonic pasteurellosis with a monovalent Pasteurella haemolytica vaccine and then infected with T. evansi TREU 2143. From 1 week post-infection (p.i.), the PBL were separated and stimulated in cultures with either Concanavalin A (Con A), bacterial lipopolysaccharide (LPS), pasteurella antigen (P.ag), or homologous trypanosome antigen (T.ag). The proliferative responses of the cells to Con A and LPS were significantly (P < 0.001) suppressed by the infection. This suppression was associated with active infection, as treatment of the sheep with a trypanocide restored the proliferative ability of the cells to both mitogens. Similarly, active infection significantly (P < 0.001) suppressed specific responses to P.ag and T.ag but although treatment resulted in full specific proliferative responsiveness to the homologous trypanosome antigen, the same was not true of P.ag, in which the responsiveness of cells from uninfected vaccinated sheep to it were still significantly higher (P < 0.001) than those of cells from infected sheep.

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“…The role of T-cells during infection of natural hosts and experimental animals with African trypanosomes has been questioned for several reasons: (1) Because of the repetitive structure of surface-exposed epitopes of the VSG, antibodies directed against these epitopes, which are responsible for clearing each parasitic wave, were considered to result from T-cell-independent B-cell responses (Mansfield 1990); (2) as trypanosomes are extracellular parasites, MHCrestricted cytotoxic T-cells are presumably not involved in their clearance; (3) more important, trypanosome antigen-specific T-cell responses have been difficult to detect in infected animals. In contrast, strong trypanosome-specific T-cell proliferation develops in cattle following infection and treatment with trypanocidal drugs (Lutje et al 1995a, Emery et al 1980.…”

Section: Introductionmentioning

confidence: 99%

Trypanosoma congolense:A Comparison of T-Cell-Mediated Responses in Lymph Nodes of Trypanotolerant and Trypanosusceptible Cattle during Primary Infection

Lutje

Taylor

Kennedy

et al. 1996

Experimental Parasitology

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Trypanosoma congolense: Specific transformation in vitro of leukocytes from infected or immunized cattle

Cited by 18 publications

References 16 publications

Susceptibility of N'Dama and Boran cattle to tsetse‐transmitted primary and rechallenge infections with a homologous serodeme of Trypanosoma congolense

Susceptibility of N'Dama and Boran cattle to tsetse‐transmitted primary and rechallenge infections with a homologous serodeme of Trypanosoma congolense

Proliferative Responses of Peripheral Blood Leucocytes of Sheep Infected with Trypanosoma evansi

Trypanosoma congolense:A Comparison of T-Cell-Mediated Responses in Lymph Nodes of Trypanotolerant and Trypanosusceptible Cattle during Primary Infection

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