Trypanosoma cervi sp. n. from Elk (Cervus canadensis) in Wyoming

Kingston, N.; Morton, Jamie

doi:10.2307/3279099

Cited by 30 publications

(22 citation statements)

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“…When the organicms were transferred back from 26 to 37 C, a larger percentage of epiniastigotes transformed into tryponiastigotes ( 7 7 % ) ( Table 3 ) . T h e growth and developinental pattern for 26 C cultures was similar to the pattern for 37 C cultures except that in the former the maximum population size (3)(4)(5)(6)(7)(8) x 106 flagellates/ml) was reached after 2 weeks instead of 4 days. Infcctir~ity.-Four of the 6 bo\~ines inoculated with trypanosornes grown in cell culture had trypanosornes in the blood within 1-6 days postinoculation.…”

Section: Trypomastigotessupporting

confidence: 61%

Continuous Cultivation of Trypanosoma theileri at 37 C in Bovine Cell Culture*

McHolland-Raymond

Kingston

Trueblood

1978

The Journal of Protozoology

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Trypanosoma theileri was cultivated at 37 C in bovine bone marrow cell culture through 50 consecutive subcultures. Medium 199, supplemented with Bacto-peptone, vitamin B12, and fetal bovine serum, was utilized both for primary and continuous cultivation. The number of trypanosomes produced in culture averaged 8 x 10(6) (1-26 x 10(6)) trypanosomes/ml. In each subculture the organisms divided as epimastigotes and transformed into trypomastigotes; a round from was observed during the stationary and declining phase of growth. Gradual changes such as increased generation time, size reduction, and decreased trypomastigote production were observed as subculturing progressed. Cultured trypanosomes were infective for the bovine through the 48th serial transfer and could be cultivated at 26 degrees C.

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Section: Trypomastigotessupporting

confidence: 61%

Continuous Cultivation of Trypanosoma theileri at 37 C in Bovine Cell Culture*

McHolland-Raymond

Kingston

Trueblood

1978

The Journal of Protozoology

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“…The dogs did not reveal any pathological symptoms of infection. Blood samples, collected from the cephalic vein into EDTAcoated tubes, were examined for the presence of microfilariae using Knott method (13), as well as Kingston and Morton's method (12). After centrifugation in haematocrit microtubes, the intensity of infection, presented as a number of microfilariae in 60 µL of blood, was determined.…”

Section: Methodsmentioning

confidence: 99%

Prevalence of Dirofilaria repens in dogs in central-eastern Poland and histopathological changes caused by this infection

Osińska

Demiaszkiewicz

Pyziel

et al. 2014

Bulletin of the Veterinary Institute in Pulawy

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The aim of the study was to determine the prevalence of canine dirofilariosis in four provinces of central-eastern Poland and to describe pathological changes occurring during the course of this parasitosis. A total of 188 dogs aged from one and a half up to 16 years were examined for dirofilariosis. Blood samples were analysed for the presence of microfilariae using Knott method, as well as the method of Kingston and Morton. Pathomorphological examination of two dogs was also performed. Histological sections were stained with H&E, AB-PAS, van Gieson, and von Kossa methods. Microfilariae of Dirofilaria repens were found in blood samples of dogs that originated from the Mazowieckie, Lubelskie, and Podlaskie provinces. The mean prevalence of that species was 12.7%. Autopsy revealed the adult nematodes in the subcutaneous connective tissue. Microscopic examinations indicated the presence of microfilariae in the liver, kidneys, lungs, heart muscle, and intestines. Necrotic foci, thrombosis, and infiltrations of different intensity with eosinophils in internal organs were also observed. Significant lesions in different internal organs together with the presence of numerous microfilariae suggest their important role in the disease process.

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“…Trypanosoma sp. identified in cervids appears to be distinct from the megatrypanosome of bovids (Trypanosoma theileri) and has been designated Trypanosoma cervi (Kingston and Morton, 1975;Kingston et al, 1982;Lefebvre et al, 1997). To date, no evidence of pathogenicity caused by infection with Megatrypanum has been noted in any cervid species (Kingston et al, 1982;Lefebvre et al, 1997).…”

Section: Parasitologymentioning

confidence: 99%

Serum Biochemistry, Serology, and Parasitology of Boreal Caribou (Rangifer Tarandus Caribou ) in the Northwest Territories, Canada

Johnson

Harms

Larter

et al. 2010

Journal of Wildlife Diseases

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ABSTRACT:Boreal caribou (Rangifer tarandus caribou) are an ecologically and culturally important wildlife species and now range almost exclusively in the boreal forests of Canada, including the Northwest Territories, northern Alberta, and British Columbia. Boreal caribou are threatened throughout their Canadian range because of direct and indirect natural and anthropogenic factors. In the Northwest Territories, however, they have a continuous range that overall has not yet been subjected to the same degree of anthropogenic habitat fragmentation and degradation that has occurred elsewhere in Canada. To monitor the health of boreal caribou populations and individuals, we collected blood from 104 adult, female boreal caribou captured between March 2003 and February 2006 and measured serum biochemical parameters. Serum creatinine was higher in pregnant than in nonpregnant caribou. Several biochemical parameters differed among years, but they tended to be similar to those reported for reindeer. Serum antibodies were found to an alphaherpesvirus, Toxoplasma gondii, and to the Mycobacterium avium subspecies paratuberculosis in 37.5, 2.9, and 1.3% of boreal caribou, respectively. Fecal samples were collected from 149 boreal caribou, and Cryptosporidium sp. oocysts, Giardia sp. cysts, trichostrongyle ova, dorsal-spined nematode larvae, cestode ova, and Eimeria sp. were found. Trypanosoma sp. was detected in the blood of 72.1% of boreal caribou. Eimeria sp., Cryptosporidium sp., and Giardia sp. have not been previously reported in boreal caribou.

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Trypanosoma cervi sp. n. from Elk (Cervus canadensis) in Wyoming

Cited by 30 publications

References 0 publications

Continuous Cultivation of Trypanosoma theileri at 37 C in Bovine Cell Culture*

Continuous Cultivation of Trypanosoma theileri at 37 C in Bovine Cell Culture*

Prevalence of Dirofilaria repens in dogs in central-eastern Poland and histopathological changes caused by this infection

Serum Biochemistry, Serology, and Parasitology of Boreal Caribou (Rangifer Tarandus Caribou ) in the Northwest Territories, Canada

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