Trypanocidal Constituents in Plants 2. Xanthones from the Stem Bark of Garcinia subelliptica

Abe, Fumiko; Nagafuji, Shinya; Okabe, Hikaru; Higo, Hiroo; Akahane, H.

doi:10.1248/bpb.26.1730

Cited by 27 publications

(34 citation statements)

References 25 publications

(44 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…10) MC 100 values of compounds 1-10 against epimastigotes and trypomastigotes were estimated by the same method as mentioned in the previous papers, 2,9) and are summarized in Table 3. Gentian violet 11) and ketoconazole 12) were also tested for comparison.…”

Section: 9)mentioning

confidence: 99%

“…2,9) Assay of Trypanocidal Activity The activity was expressed as the MC 100 value (mg/ml or mM), which was defined as the minimum concentration at which all the epimastigotes or trypomastigotes become immobilized after 24 h incubation. 10) MC 100 values of compounds 1-10 against epimastigotes and trypomastigotes were estimated by the same method as mentioned in the previous papers, 2,9) and are summarized in Table 3. Gentian violet 11) and ketoconazole 12) were also tested for comparison.…”

Section: Cultivation Of T Cruzimentioning

confidence: 99%

“…Cell Counting Kit-8 In previous papers, 1,2,9) we assayed trypanocidal activity by observing the mobility of T. cruzi microscopically. The activity was shown by MC 100 value, which was defined as the minimum concentration at which all the epimastigotes or trypomastigotes become immobilized after 24 or 48 h incubation.…”

Section: Evaluation Of Ic 50 Values Against Epimastigotes Usingmentioning

confidence: 99%

“…

…”

mentioning

confidence: 99%

See 3 more Smart Citations

Trypanocidal Constituents in Plants: 4. Withanolides from the Aerial Parts of Physalis angulata

Nagafuji

Okabe

Akahane

et al. 2004

Biological & Pharmaceutical Bulletin

Self Cite

View full text Add to dashboard Cite

In the previous paper in this series, 2) we described the identification of trypanocidal xanthones in Garcinia subelliptica against epimastigotes and trypomastigotes of the protozoan Trypanosoma cruzi, which is the etiologic agent of Chagas' disease (American trypanosomiasis). In the course of our search for trypanocidal constituents in plants, we have found that the MeOH extract of the aerial parts of Physalis angulata L. (Solanaceae) showed considerable activity.P. angulata is indigenous to tropical America and was naturalized in Japan in the Edo era. It is an annual small herb and has been traditionally utilized for antipyretic purposes in Japan.3) The isolation of ergostane-type steroidal compounds, physagulins A-G, 4-6) withangulatin A 7) and physalins, 8) has been reported from this plant.T. cruzi is transformed into three stages in its life cycle: epimastigote in the insect gut; trypomastigote, an infectious form in the mammalian blood stream; and amastigote, a proliferative form in mammalian cells. During the isolation procedure, trypanocidal activity was monitored against epimastigotes, because it is easy to cultivate them and they are not infectious. Ten withanolides (1-10) were isolated from the active fraction. To check the therapeutic possibility of these withanolides, trypanocidal activity against trypomastigotes, an infectious form, was examined. Furthermore, evaluation of the IC 50 value against epimastigotes was performed using Cell Counting Kit-8 in order to compare with cytotoxicity at the IC 50 value. In this paper we describe the structural elucidation of the four new withanolides (2, 5, 9, 10) and the trypanocidal activity of the isolates. The possibility of chemotherapeutic utility is also discussed. MATERIALS AND METHODSPlant Materials P. angulata was cultivated in the medicinal plant garden of Fukuoka University and harvested in September 2002. The aerial parts were air-dried and powdered.Instruments and Reagents The instruments used in this study were a JEOL JNM-A500 for NMR spectra (500 MHz for 1 H-NMR, 125 MHz for 13 C-NMR), a JEOL JMS-HX 110 for MS spectra, a JASCO DIP 360 polarimeter for optical rotations, and a Labsystems Multiskan JX Microplate Reader for measuring the absorbance of a formazan product. The following materials were used for chromatography: Diaion HP-20 (a styrene polymer, Mitsubishi Chemical Industries, Ltd.), Silica gel 60N (Cica), Sephadex LH-20 (Pharmacia Fine Chemicals), and YMC gel (ODS, YMC Co. Ltd.). Newborn calf serum (NCS) was purchased from Nacalai Tesque. Dulbecco's modified Eagle's medium (DMEM) and RPMI 1640 medium were purchased from Sigma. Gentian violet, ketoconazole, and 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were purchased from Wako. A Cell Counting Kit-8 was purchased from Dojindo. Other reagents are the same as in the previous papers. 2,9)Cultivation of T. cruzi The strain of T. cruzi used in this study and the cultivation methods of epimastigotes and trypomastigotes were the same as those described in the previous p...

show abstract

Section: 9)mentioning

confidence: 99%

Section: Cultivation Of T Cruzimentioning

confidence: 99%

Section: Evaluation Of Ic 50 Values Against Epimastigotes Usingmentioning

confidence: 99%

“…

…”

mentioning

confidence: 99%

See 2 more Smart Citations

Trypanocidal Constituents in Plants: 4. Withanolides from the Aerial Parts of Physalis angulata

Nagafuji

Okabe

Akahane

et al. 2004

Biological & Pharmaceutical Bulletin

Self Cite

View full text Add to dashboard Cite

show abstract

“…Compounds isolated from Garcinia subelliptica (Guttiferae) displayed trypanocidal activity against trypomastigotes and epimastigotes of T. cruzi 47 . Although in this mentioned study fukugetin showed no activity in vivo, our results demonstrated fukugetin was a potent inhibitor of cruzain and parasites extracts with concentrations in nonetheless in the low micromolar range.…”

Section: Discussionmentioning

confidence: 99%

Inhibition of cysteine proteases by a natural biflavone: behavioral evaluation of fukugetin as papain and cruzain inhibitor

Assis

Gontijo

Pereira

et al. 2012

Journal of Enzyme Inhibition and Medicinal Chemistry

View full text Add to dashboard Cite

A New Benzoylphloroglucinol Derivative with an Adamantyl Skeleton and Other Constituents from Garcinia multiflora: Effects on Neutrophil Pro‐Inflammatory Responses

Ting¹,

Hwang²,

Chen³

et al. 2012

Chemistry & Biodiversity

View full text Add to dashboard Cite

A novel benzoylphloroglucinol derivative, garcimultiflorone D (1), with an unusual adamantyl-caged skeleton was isolated from the fruits of Garcinia multiflora, together with four known compounds. The structure of 1 was determined through extensive 1D/2D-NMR and mass-spectrometric analyses. Garcimultiflorone D (1) exhibited inhibitory activities with IC(50) values of 7.21±1.07 and 6.01±0.37 μg/ml against fMLP/CB-induced superoxide anion generation and elastase release, respectively.

show abstract

Trypanocidal Constituents in Plants 2. Xanthones from the Stem Bark of Garcinia subelliptica

Cited by 27 publications

References 25 publications

Trypanocidal Constituents in Plants: 4. Withanolides from the Aerial Parts of Physalis angulata

Trypanocidal Constituents in Plants: 4. Withanolides from the Aerial Parts of Physalis angulata

Inhibition of cysteine proteases by a natural biflavone: behavioral evaluation of fukugetin as papain and cruzain inhibitor

A New Benzoylphloroglucinol Derivative with an Adamantyl Skeleton and Other Constituents from Garcinia multiflora: Effects on Neutrophil Pro‐Inflammatory Responses

Contact Info

Product

Resources

About