Trypanocidal and leishmanicidal activities of different antimicrobial peptides (AMPs) isolated from aquatic animals

Löfgren, Sara Emelie; Miletti, Luiz Cláudio; Steindel, Mário; Bachère, Evelyne; Barracco, Margherita Anna

doi:10.1016/j.exppara.2007.07.011

Cited by 101 publications

(61 citation statements)

References 30 publications

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“…The results were determined by the percentage of hemolysis compared with the results obtained using the negative and positive controls. 44 The results were expressed as the mean percentage reduction in O + human red blood cells compared with non-treated control wells, and represented by the 50% hemolytic concentration (RBC 50 ).…”

Section: Hemolytic Activitymentioning

confidence: 99%

Novel targeting using nanoparticles: an approach to the development of an effective anti-leishmanial drug-delivery system

Ribeiro

Chávez-Fumagalli

Valadares

et al. 2014

IJN

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The study reported here aimed to develop an optimized nanoparticle delivery system for amphotericin B (AmpB) using a polyelectrolyte complexation technique. For this, two oppositely charged polymers presenting anti-leishmanial activity – chitosan (Cs) and chondroitin sulfate (ChS) – were used: Cs as a positively charged polymer and ChS as a negatively charged polymer. The chitosan (NQ) nanoparticles, chitosan-chondroitin sulfate (NQC) nanoparticles, and chitosan-chondroitin sulfate-amphotericin B (NQC-AmpB) nanoparticles presented a mean particle size of 79, 104, and 136 nm, respectively; and a polydispersity index of 0.2. The measured zeta potential of the nanoparticles indicated a positive charge in their surface, while scanning and transmission electron microscopy revealed spherical nanoparticles with a smooth surface. Attenuated total reflectance-Fourier transform infrared spectroscopy analysis showed an electrostatic interaction between the polymers, whereas the release profile of AmpB from the NQC-AmpB nanoparticles showed a controlled release. In addition, the Cs; ChS; and NQ, NQC, and NQC-AmpB nanoparticles proved to be effective against promastigotes of Leishmania amazonensis and Leishmania chagasi , with a synergistic effect observed between Cs and ChS. Moreover, the applied NQ, NQC, and NQC-AmpB compounds demonstrated low toxicity in murine macrophages, as well as null hemolytic activity in type O + human red blood cells. Pure AmpB demonstrated high toxicity in the macrophages. The results show that cells infected with L. amazonensis and later treated with Cs, ChS, NQ, NQC, NQC-AmpB nanoparticles, or pure AmpB presented with a significant reduction in parasite number in the order of 24%, 31%, 55%, 66%, 90%, and 89%, respectively. The data presented indicate that the engineered NQC-AmpB nanoparticles could potentially be used as an alternative therapy to treat leishmaniasis, mainly due its low toxicity to mammals’ cells.

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Section: Hemolytic Activitymentioning

confidence: 99%

Novel targeting using nanoparticles: an approach to the development of an effective anti-leishmanial drug-delivery system

Ribeiro

Chávez-Fumagalli

Valadares

et al. 2014

IJN

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“…Development of microbial resistance to HDPs is considered to be uncommon or less likely than to conventional antibiotics, ostensibly reflecting the significant changes in structure and phospholipid composition that would be necessary to prevent peptide interactions (18). The diversity of HDPs and their targets has been well documented, and many specific peptides have been demonstrated to have both in vitro and in vivo antiprotozoan activity in prior studies (1,29,30), including plant HDPs (3), various animal-derived HDPs (4,9,15,22,26,33,34), human salivary HDPs (24), and defensin-, magainin-, and cathelicidin-type HDPs (12,21,25).…”

mentioning

confidence: 99%

Efficacy of Synthetic Peptides RP-1 and AA-RP-1 against Leishmania Species In Vitro and In Vivo

Erfe

David

Huang

et al. 2012

Antimicrob Agents Chemother

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Host defense peptides are naturally occurring molecules that play essential roles in innate immunity to infection. Based on prior structure-function knowledge, we tested two synthetic peptides (RP-1 and AA-RP-1) modeled on the conserved, microbicidal ␣-helical domain of mammalian CXCL4 platelet kinocidins. These peptides were evaluated for efficacy against Leishmania species, the causative agents of the group of diseases known as leishmaniasis. In vitro antileishmanial activity was assessed against three distinct Leishmania strains by measuring proliferation, metabolic activity and parasite viability after exposure to various concentrations of peptides. We demonstrate that micromolar concentrations of RP-1 and AA-RP-1 caused dose-dependent growth inhibition of Leishmania promastigotes. This antileishmanial activity correlated with rapid membrane disruption, as well as with a loss of mitochondrial transmembrane potential. In addition, RP-1 and AA-RP-1 demonstrated distinct and significant in vivo antileishmanial activities in a mouse model of experimental visceral leishmaniasis after intravenous administration. These results establish efficacy of RP-1 lineage synthetic peptides against Leishmania species in vitro and after intravenous administration in vivo and provide further validation of proof of concept for the development of these and related systemic antiinfective peptides targeting pathogens that are resistant to conventional antibiotics.

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“…This probably leads to permeabilization and disruption of bacterial plasma membranes [166,167]. More recent studies confirmed the antibacterial activity of tachyplasins [168,169] and showed that these compounds also exhibit antiviral [170], antiparasitic [171], and anticancer effects [172].…”

Section: Horseshoe Crab-derived Antibioticsmentioning

confidence: 75%

Exploring the global animal biodiversity in the search for new drugs – Spiders, scorpions, horseshoe crabs, sea spiders, centipedes, and millipedes

Mans¹

2017

J Transl Sci

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Drug discovery and development programs have historically mainly focused on plants with medicinal properties and the extensive knowledge of traditional healers about these plants. More recently, the vast array of marine invertebrates and insects throughout the world have been recognized as additional sources for identifying unusual lead compounds to obtain structurally novel and mechanistically unique therapeutics. The results from these efforts are encouraging and have yielded a number of clinically useful drugs. However, many arthropods other than insects -such as spiders, scorpions, horseshoe crabs, sea spiders, centipedes, and millipedes -also produce hundreds of bioactive substances in their venom that may become useful in the clinic. Many of these chemicals are defensive and predatory weapons of these creatures and have been refined during millions of years of evolution to rapidly and with high specificity and high affinity shut down critical molecular targets in prey and predators. Exploration of these compounds may lead to the development of, among others, novel drugs for treating diseases caused by abnormalities in humans in the same (evolutionary conserved) molecular targets such as erectyle dysfunction, botulism, and autoimmune disorders, as well as the identification of novel antineoplastic, antimicrobial, and antiparasitic compounds. This paper addresses the importance of bioactive compounds from spiders, scorpions, horseshoe crabs, sea spiders, centipedes, and millipedes to these advances.

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Trypanocidal and leishmanicidal activities of different antimicrobial peptides (AMPs) isolated from aquatic animals

Cited by 101 publications

References 30 publications

Novel targeting using nanoparticles: an approach to the development of an effective anti-leishmanial drug-delivery system

Novel targeting using nanoparticles: an approach to the development of an effective anti-leishmanial drug-delivery system

Efficacy of Synthetic Peptides RP-1 and AA-RP-1 against Leishmania Species In Vitro and In Vivo

Exploring the global animal biodiversity in the search for new drugs – Spiders, scorpions, horseshoe crabs, sea spiders, centipedes, and millipedes

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