Trypanocidal activity of flavonoids and limonoids isolated from Myrsinaceae and Meliaceae active plant extracts

Abstract

RESUMO: "Atividade tripanocida de flavonoides e limonoides isolados de extratos ativos de plantas de Myrsinaceae e Meliaceae". A atividade de extratos brutos de três espécies de Rapanea (Myrsinaceae) e de Cipadessa fruticosa (Meliaceae) foi avaliada in vitro contra formas tripomastigotas de Trypanosoma cruzi. Foram obtidos 33 extratos de diferentes órgãos das espécies estudadas, sendo que onze deles apresentaram atividades significantes (% de lise > 50) nos ensaios realizados. O fracionamento de um extrato ativo dos galhos de R. lancifolia (99,5%) resultou no isolamento de dois flavonoides (quercetina e taxifolina), que apresentaram baixa atividade tripanocida. De um extrato ativo dos frutos de C. fruticosa (97,7%) foram isolados os limonoides mexicanolídeos cipadesina, mexicanolídeo, febrifugina e cipadesina A, que foram moderadamente ativos sobre T. cruzi. Além disso, outros dois flavonoides (flavona e 7-metoxiflavona), previamente ensaiados contra T. cruzi, foram isolados do extrato hexânico dos galhos de C. fruticosa (100%). Os resultados obtidos aqui sugerem que as plantas avaliadas podem constituir fontes de novas substâncias ativas sobre o T. cruzi.Unitermos: Myrsinaceae, Meliaceae, atividade tripanocida, flavonoides, limonoides. ABSTRACT:The activity of crude extracts of three Rapanea species (Myrsinaceae) and Cipadessa fruticosa (Meliaceae) was evaluated in vitro against the trypomastigote forms of Trypanosoma cruzi. Thirty-three extracts from different organs of these species were assayed and eleven of them showed significant activity (lysis % >50). The fractionation of an active extract from branches of R. lancifolia (99.5%) led to the isolation of two flavonoids: quercetin and taxifolin, which have weak trypanocidal activity. Additionally, one active extract from fruits of C. fruticosa (97.7%) afforded mexicanolide limonoids: cipadesin, mexicanolide, febrifugin and cipadesin A, that were slightly active on T. cruzi. Moreover, other two flavonoids (flavone and 7-methoxyflavone), previously assayed against T. cruzi, were isolated from the hexane extract from branches of C. fruticosa (100%). The results presented here suggest that the plants evaluated could be a source of new active compounds against T. cruzi.

In Vitro anti-leismanial activity of Malaysian medicinal and forest plant species

“…Leite et al (2010) reported trypanocidal activity from isolated limonoids while Ambrozin et al (2005) suggested a novel drug for leishmaniasis as seen by the significant inhibition of the extracts on enzyme adenine phosphoribosyltransferase (APRT) from Leishmania. No reports were found on antileishmanial from other species (Anisophylleaceae and Arecaceae) which were found active in this study.…”

Pharmacognostic Characterization, Bioactive Compounds and Powder Antioxidant Action of Leaves of Araca (Psidium cattleianum myrtaceae)

“…The secondary metabolites are defined as bioactive compounds that whose function is to adapt the plant to the environment, performing important functions such as defense against infection, injury, pests, pathogens, ultraviolet rays and herbivores [2][3][4]. These classes of substances, frequently, are targets of interest of researchers who see them as a promising source for discovery of new drugs, cosmetics and agrochemicals [5][6][7].…”