Trypan Blue Capsulorhexis

This article aims to evaluate the outcomes of torsional and burst mode phacoemulsification in hard nuclear cataracts. Eighty eyes with grade IV or V nuclear opalescence were treated with phacoemulsification and intraocular lens implantation using conventional mode (Group A, n = 40) or torsional and burst mode phacoemulsification (Group B, n = 40). For good visualization of anterior capsule, trypan blue was injected to the anterior chamber before continuous circular capsulorhexis. The mean cumulative dissipated energy and ultrasound time were recorded. The best-corrected visual acuity, endothelial cell density, and central corneal thickness were measured before and at 1 month after surgery. The cumulative dissipated energy and ultrasound time of Group B were significantly less than that of Group A. The postoperative best-corrected visual acuities of the 2 groups were comparable. At 1 month after surgery, the changes in the endothelial cell density were significantly greater in Group A than in Group B, and the changes in the central corneal thickness were not significantly different between the 2 groups. Torsional and burst mode is a safe and effective surgical method for treating hard cataracts.

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“…Although this procedure may increase the operative time, it can benefit capsulorhexis and subsequent manipulations greatly. [12]…”

Section: Discussionmentioning

confidence: 99%

Torsional and burst mode phacoemulsification for patients with hard nuclear cataract

et al. 2019

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“…To optimize TB staining of biofilms on chicken relative to the background tissue, a preliminary experiment was conducted to evaluate the impact of different TB concentrations (0.01, 0.04, 0.1, 0.4% w / v ) and incubation times (10, 20, 40 min) based on parameters reported in the literature [ 19 , 24 , 28 , 31 ]. In all the cases, the chicken samples in 24-well plates were completely submerged in the TB solution for the indicated time span.…”

Section: Experimental Setup and Proceduresmentioning

confidence: 99%

“…However, transfer of the sample to the nitrocellulose membrane may result in further undesirable spreading of the biofilms over the wound surface. trypan blue (TB) has also been explored as a biofilm selective dye for imaging [ 18 , 19 , 20 ]. TB was initially used in the 19th century as a textile dye and compound to treat trypanosomal infections [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

“…TB was initially used in the 19th century as a textile dye and compound to treat trypanosomal infections [ 21 ]. TB has also been explored over the years as a dye to stain tissues during biopsies, or to stain living cells and organisms [ 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 ]. In an image-guided surgery study, Chang et al assessed the various concentrations of TB required to effectively stain a rabbit anterior eye capsule and found that a 0.1% TB ( w / v ) solution was sufficient to identify the eye capsule [ 26 ].…”

Section: Introductionmentioning

confidence: 99%

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Selective Optical Imaging for Detection of Bacterial Biofilms in Tissues

et al. 2023

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Significance: The development of an imaging technique to accurately identify biofilm regions on tissues and in wounds is crucial for the implementation of precise surface-based treatments, leading to better patient outcomes and reduced chances of infection. Aim: The goal of this study was to develop an imaging technique that relies on selective trypan blue (TB) staining of dead cells, necrotic tissues, and bacterial biofilms, to identify biofilm regions on tissues and wounds. Approach: The study explored combinations of ambient multi-colored LED lights to obtain maximum differentiation between stained biofilm regions and the underlying chicken tissue or glass substrate during image acquisition. The TB imaging results were then visually and statistically compared to fluorescence images using a shape similarity measure. Results: The comparisons between the proposed TB staining method and the fluorescence standard used to detect biofilms on tissues and glass substrates showed up to 97 percent similarity, suggesting that the TB staining method is a promising technique for identifying biofilm regions. Conclusions: The TB staining method demonstrates significant potential as an effective imaging technique for the identification of fluorescing and non-fluorescing biofilms on tissues and in wounds. This approach could lead to improved precision in surface-based treatments and better patient outcomes.

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Trypan Blue Capsulorhexis

Cited by 2 publications

References 4 publications

Torsional and burst mode phacoemulsification for patients with hard nuclear cataract

Torsional and burst mode phacoemulsification for patients with hard nuclear cataract

Selective Optical Imaging for Detection of Bacterial Biofilms in Tissues

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