Trx CDSP32-overexpressing tobacco plants improves cadmium tolerance by modulating antioxidant mechanism

Yao, Tongtong; Wang, Yue; Wang, Jiechen; Song, Jiaqi; Cui, Congcong; Ji, Guangxin; Cao, Jianhua; Salman, Muhammad; Ao, Hong Rui; Zhang, Huihui

doi:10.1016/j.plaphy.2022.11.036

Cited by 11 publications

(2 citation statements)

References 51 publications

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“…Trxs distribute the reductant to selected targets via a dithiol-disulfide exchange, resulting in the structural and functional modification of a target protein (Balsera and Buchanan, 2019). Zhang et al reported that the thioredoxin-like protein CDSP32 improves cadmium tolerance by alleviating Cd-induced photosynthetic inhibition (Zhang et al, 2020) and modulating antioxidant system in tobacco leaves (Zhang et al, 2023b). The Glutaredoxin (Grx) cycle, comprised of Grxs, GSH, and glutathione reductase (GR), is crucial for maintaining intracellular redox homeostasis (Ogata et al, 2021).…”

Section: Degs Involved In Antioxidant Systemmentioning

confidence: 99%

Transcriptome analysis reveals ABA involved in the detoxification mechanism of macroalga Gracilariopsis lemaneiformis to cadmium toxicity

Chen,

Tang,

Sun

et al. 2024

Front. Mar. Sci.

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IntroductionCadmium (Cd) is a significant threat environmental pollutant in the marine ecological environment offshore. The macroalga, Gracilariopsis lemaneiformis, of significant economic value, is widely cultivated along China’s coastline. Yet, little is known about the molecular mechanisms underlying Cd tolerance in macroalga.MethodsHere, we examined the transcriptome of G. lemaneiformis exposed to Cd to identify the responses to Cd stress.Results and discussionOur findings revealed that Cd led to the retardation of growth rate in G. lemaneiformis, accompanied by a notable reduction in the content of photosynthetic pigments and a decrease in the expression of genes associated with the photosynthetic system and nitrogen metabolism. When exposed to Cd, there was a rapid increase in Cd levels through the upregulation of genes encoding GlZIP6 and GlIRT1. Additionally, the expression of Cd efflux transporters, GlZIP1 and GlABCG22, and the ABCC7 transporter for compartmentation to the vacuole, was induced to mitigate Cd toxicity. Cd also activated crucial genes involved in the ABA biosynthesis and enhanced ABA content, thereby inducing ABA signaling pathway. Furthermore, exogenous ABA reduced the growth inhibition of G. lemaneiformis under Cd stress. Redox homeostasis was adjusted to adapt to Cd toxicity, with thioredoxin, glutaredoxin cycle and ascorbate-glutathione cycle identified as playing significant in maintaining reactive oxygen species homeostasis. Moreover, transcription factors such as several MYBs, signal transmission factors G protein and heat shock proteins (sHSPs, HSP 40, HSP 90, HSP101) were involved in the detoxification of Cd. Collectively, this study provided a comprehensive understanding of the molecular mechanisms underpinning the of responses of macroalga G. lemaneiformis to Cd exposure.

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Section: Degs Involved In Antioxidant Systemmentioning

confidence: 99%

Transcriptome analysis reveals ABA involved in the detoxification mechanism of macroalga Gracilariopsis lemaneiformis to cadmium toxicity

Chen,

Tang,

Sun

et al. 2024

Front. Mar. Sci.

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“…It is known that CAT is involved in converting reactive H 2 O 2 and O 2 to benign H 2 O [27]. Cd can also replace Fe in the active site and further inhibit the activity of CAT, causing the overproduction of ROS and thus inducing cell oxidative damage [28,29]. However, IlHMA2-expressing tobacco significantly inhibited the decrease in CAT activity relative to WT plants, accompanied by the reduction in the level of H 2 O 2 and MDA.…”

Section: Overexpression Of Ilhma2 Contributed To Scavenging the Ros I...mentioning

confidence: 99%

Overexpression of IlHMA2, from Iris lactea, Improves the Accumulation of and Tolerance to Cadmium in Tobacco

Li,

Wang,

Hou

et al. 2023

Plants

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Long-distance transport cadmium (Cd) from roots to shoots is a key factor for Cd phytoremediation. Our previous study indicated that heavy metal P1B2-ATPases, IlHMA2, was involved in improving the accumulation of Cd via mediated long-distance transport Cd, contributing to the phytoremediation in Cd accumulator Iris lactea. However, whether the overexpression of IlHMA2 could enhance the accumulation and tolerance to Cd remains unclear in plants. Here, we generated transgenic tobacco overexpressing IlHMA2 and tested its effect on the translocation and accumulation of Cd and zinc (Zn), as well as the physio-biochemical characteristics under 50 mg/L Cd exposure. The overexpression of IlHMA2 significantly increased Cd concentrations in xylem saps, resulting in enhanced root-to-shoot Cd translocation compared with wild-type. Meanwhile, overexpressing IlHMA2 promoted Zn accumulations, accompanied by elevating proline contents and antioxidant enzyme activity (SOD, POD, and CAT) to diminish the overproduction of ROS in transgenic tobacco. These pieces of evidence suggested that higher Zn concentrations and lower ROS levels could tremendously alleviate Cd toxicity for transgenic tobacco, thereby improving the growth and tolerance. Overall, the overexpression of IlHMA2 could facilitate Cd accumulation and enhance its tolerance in tobacco exposed to Cd contaminations. This would provide a valuable reference for improving Cd phytoremediation efficiency.

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Antioxidant Defence: A Key Frontier in Conferring Cadmium Tolerance in Plants

Biswas,

Pal

2024

Springer Water

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Trx CDSP32-overexpressing tobacco plants improves cadmium tolerance by modulating antioxidant mechanism

Cited by 11 publications

References 51 publications

Transcriptome analysis reveals ABA involved in the detoxification mechanism of macroalga Gracilariopsis lemaneiformis to cadmium toxicity

Transcriptome analysis reveals ABA involved in the detoxification mechanism of macroalga Gracilariopsis lemaneiformis to cadmium toxicity

Overexpression of IlHMA2, from Iris lactea, Improves the Accumulation of and Tolerance to Cadmium in Tobacco

Antioxidant Defence: A Key Frontier in Conferring Cadmium Tolerance in Plants

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