Truncated light-harvesting chlorophyll antenna size in Chlorella vulgaris improves biomass productivity

Shin, Wonsub; Lee, Bongsoo; Jeong, Byeong‐ryool; Chang, Yong Keun; Kwon, Jong-Hee

doi:10.1007/s10811-016-0874-8

Cited by 83 publications

(66 citation statements)

References 32 publications

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“…NsbZIP1 transformants showed higher Fv/Fm ( Figure S3) under all culture conditions, indicating that better photosynthetic efficiency compared to WT. It seems that NsbZIP1 improved photosynthetic efficiency resulting in enhanced growth of the transformants as reported for other microalgae (Perin et al, 2015;Shin, Lee, Jeong, Chang, & Kwon, 2016).…”

Section: Growth Of Nsbzip1 Transformants Under Various Culture Condsupporting

confidence: 72%

Enhancement of biomass and lipid productivity by overexpression of a bZIP transcription factor in Nannochloropsis salina

Kwon

Kang

Koh

et al. 2017

Biotech & Bioengineering

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Microalgae are considered as excellent platforms for biomaterial production that can replace conventional fossil fuel-based fuels and chemicals. Genetic engineering of microalgae is prerequisite to maximize production of materials and to reduce costs for the production. Transcription factors (TFs) are emerging as key regulators of metabolic pathways to enhance production of molecules for biofuels and other materials. TFs with the basic leucine zipper (bZIP) domain have been known as stress regulators and are associated with lipid metabolism in plants. We overexpressed a bZIP TF, NsbZIP1, in Nannochloropsis salina, and found that transformants showed enhanced growth with concomitant increase in lipid contents. The improved phenotypes were also notable under stress conditions including N limitation and high salt. To understand the mechanism underlying improved phenotypes, we analyzed expression patterns of predicted target genes involved in lipid metabolism via quantitative RT-PCR, confirming increases transcript levels. NsbZIP1 appeared to be one of type C bZIPs in plants that has been known to regulate lipid metabolism under stress. Taken together, we demonstrated that NsbZIP1 could improve both growth and lipid production, and TF engineering can serve as an excellent genetic engineering tool for production of biofuels and biomaterials in microalgae.

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Section: Growth Of Nsbzip1 Transformants Under Various Culture Condsupporting

confidence: 72%

Enhancement of biomass and lipid productivity by overexpression of a bZIP transcription factor in Nannochloropsis salina

Kwon

Kang

Koh

et al. 2017

Biotech & Bioengineering

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“…This route leads to a reduction in the activity of photosystem II, decreasing the production of oxygen and thus favoring the activity of the hydrogenase. Parallel to this, the effect of light selfattenuation also decreases [84][85][86]. To carry out the process on a large scale, some authors have proposed to limit the entry of sulphate to the chloroplast by creating mutant strains with altered activity of the sulphate permease at the chloroplast envelope.…”

Section: Hydrogenmentioning

confidence: 99%

“…To carry out the process on a large scale, some authors have proposed to limit the entry of sulphate to the chloroplast by creating mutant strains with altered activity of the sulphate permease at the chloroplast envelope. [86,87].…”

Section: Hydrogenmentioning

confidence: 99%

Microalgae biorefineries: applications and emerging technologies

Giraldo

Romo-Buchelly

Arbeláez-Pérez

et al. 2018

DYNA

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Microalgae transform CO2 into a broad portfolio of biomolecules, and thus should be considered as a valuable biotechnological platform. Despite several research programs and global efforts to establish a sustainable microalgae-based industry, most applications have not transcended academic boundaries. This limitation raises from the high transformation costs when target products are biofuels or fertilizers. Microalgae biorefinery emerges as an alternative to improve economic competitiveness, as Under such a model scope, the process inputs come from industrial waste, while biomass exploitation prioritizes the highest value molecules followed by extraction of less valuable compounds. This review describes a wide range of microalgae exploitation schemes focused on new uses of its constituents, while discussing emerging technologies designed to improve production and efficiencies as well.Keywords: microalgae; biorefinery; biofuels; fertilizers; active biomolecules. Biorefinerías de microalgas: aplicaciones actuales y nuevas tecnologías en desarrolloResumen Las microalgas transforman el CO2 en un amplio portafolio de biomoléculas, por lo cual, son consideradas una valiosa plataforma biotecnológica. A pesar de múltiples programas de investigación y esfuerzos globales para establecer una industria sostenible basada en microalgas, la mayoría de las aplicaciones potenciales no han trascendido las fronteras académicas. Esta limitación se debe a los altos costos en la transformación del producto principalmente cuando se obtiene compuestos económicos como biocombustibles y fertilizantes. La biorefinería de microalgas surge como alternativa para incrementar la competitividad económica. En este modelo, los insumos del proceso provienen de residuos industriales, mientras que la explotación de la biomasa inicia con las moléculas de alto valor y finaliza con los compuestos menos valiosos. En esta revisión se describe un amplio abanico de esquemas de explotación de microalgas enfocado en nuevos usos de sus constituyentes. Además, se exploran las tecnologías emergentes destinadas a aprovechar esta biomasa de una manera más versátil y eficiente.

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“…al. 2003], faster growth [Mussgnug et al 2007], and better biomass productivity in laboratory conditions have been reported [Beckman et al 2009, Shin et al 2016, Shin et al 2017. Several strains that appeared promising based on laboratory performance did not show improved biomass productivity in mass culture conditions simulated in laboratory-scale panel photobioreactors, possibly due to unintended effects of the genetic modifications or higher vulnerability to photodamage [De Mooij et al 2015].…”

Section: Introductionmentioning

confidence: 98%

“…Numerous efforts have been made in chlorophytes to reduce the size of photoantennae, which serve to capture light energy and funnel it to the photosynthetic reaction centers. This has generally resulted in improved photosynthetic parameters, such as saturation of photosynthesis at higher irradiance, greater light-saturated rates of oxygen evolution on a per-chlorophyll basis [Beckmann et al 2009, Cazzaniga et al 2014, Kirst et al 2012a, Kirst et al 2012b, Mitra and Melis 2008, Nakajima et al 2001, Shin et al 2016, Shin et al 2017, increased quantum yield and lower photoinhibition [Mussgnug et al 2007]. In some cases, higher maximal culture density [Polle at.…”

Section: Introductionmentioning

confidence: 99%

Enhanced triacylglycerol (TAG) and protein accumulation in transgenic diatomThalassiosira pseudonanawith altered photosynthetic pigmentation

Gaidarenko

Yee

Hildebrand

2020

Preprint

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Microalgal productivity in mass cultures is limited by the inefficiency with which available light energy is utilized. In dense cultures, cells closest to the light source absorb more light energy than they can use and dissipate the excess, while light penetrance into the culture is steeply attenuated. Reducing microalgal light harvesting and/or dissipating capacity per cell may improve total light utilization efficiency in mass cultures. In this study, two transgenic lines of the diatom Thalassiosira pseudonana with altered photosynthetic pigment content are evaluated with respect to photosynthetic parameters, growth, and macromolecule accumulation. In one line, violaxanthin de-epoxidase-like 2 (VDL2) is overexpressed (OE), resulting in a reduction of the diadinoxanthin cycle pigments, which are involved in light energy dissipation (non-photochemical quenching, NPQ), accompanied by a stoichiometric increase in the light-harvesting pigment fucoxanthin. No differences in the maximum potential quantum yield of photosystem II (Fv/Fm) or light-limited photosynthetic rate (α) were found. However, when adapted to 30 µmol photons m -2 sec -1 , the VDL2 OE maximum relative electron transport rate (rETRmax) upon exposure to saturating light intensities was 86-95% of wild type (WT). When adapted to 300 µmol photons m -2 sec -1 , VDL2 OE saturated photosynthesis at 62-71% of the light intensity needed to saturate WT (Ek). NPQ was substantially lower at and below 300 µmol photons m -2 sec -1 . VDL2 OE accumulated up to 3.4 times as much triacylglycerol (TAG) as WT during exponential growth, and up to twice as much protein. Growth in terms of culture density was up to 7% slower. TAG and protein accumulation inversely correlated with NPQ. The second line evaluated was obtained by using antisense RNA to simultaneously silence or knock down (KD) both LUT1-like (LTL) genes, hypothesized to catalyze an intermediate carotenoid biosynthesis step of converting β-carotene to zeaxanthin. Overall reduction of photosynthetic pigment content without altering the relative abundance of individual pigments resulted. No significant differences in photosynthetic parameters compared to WT were found. LTL KD grew at a rate comparable to WT and accumulated up to 40% more TAG during exponential growth, while protein content was reduced by 11-19%. LTL KD cells were elongated and 5-10% smaller than WT, and cultures contained auxospores, indicating stress that may relate to a cell cycle progression defect.Conception and design (OG, MH), experimental work (OG, DPY), data analysis and interpretation (OG), drafting of the manuscript (OG), critical revision for important intellectual content and final approval (OG, DPY). MH is deceased.

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Truncated light-harvesting chlorophyll antenna size in Chlorella vulgaris improves biomass productivity

Cited by 83 publications

References 32 publications

Enhancement of biomass and lipid productivity by overexpression of a bZIP transcription factor in Nannochloropsis salina

Enhancement of biomass and lipid productivity by overexpression of a bZIP transcription factor in Nannochloropsis salina

Microalgae biorefineries: applications and emerging technologies

Enhanced triacylglycerol (TAG) and protein accumulation in transgenic diatomThalassiosira pseudonanawith altered photosynthetic pigmentation

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