True one cell chemical analysis: a review

Silva, Imesha W. De; Kretsch, Amanda; Lewis, Holly-May; Bailey, Melanie J.; Verbeck, Guido F.

doi:10.1039/c9an00558g

Cited by 11 publications

(7 citation statements)

References 124 publications

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“…[1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17] This is because bulk measurements of populations of cells does not identify heterogeneity, which is a fundamental property of all biological systems and has wide ranging consequences including for cell to cell communication, treatment of infectious diseases and cancers. 1 Therefore, analysing single cells is crucial to answering fundamental biological questions and has wide reaching impact including in drug discovery applications. 2 We must therefore develop sensitive and reliable methods to probe single cells.…”

Section: Introductionmentioning

confidence: 99%

Nanocapillary sampling coupled to liquid chromatography mass spectrometry delivers single cell drug measurement and lipid fingerprints

Lewis

Gupta

Saunders

et al. 2023

Analyst

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Section: Introductionmentioning

confidence: 99%

Nanocapillary sampling coupled to liquid chromatography mass spectrometry delivers single cell drug measurement and lipid fingerprints

Lewis

Gupta

Saunders

et al. 2023

Analyst

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“…Similar to infrared spectroscopy technology commonly used for cell analysis, RS can be used for exploring the information about cellular molecular structure, composition, and intermolecular interaction via the vibrations or rotations of the molecule. Furthermore, RS holds unique advantages over traditional infrared spectroscopy, such as higher spatial resolution and less absorption to water, enabling it to offer more cellular biochemical information as well as to greatly avoid the interference from intercellular or intracellular water during cell analysis [15]. Besides, RS is superior to fluorescence spectroscopy in biomedical applications, owing to its narrow bands generated by various biomolecules [16].…”

Section: Introductionmentioning

confidence: 99%

Study on the chemodrug-induced effect in nasopharyngeal carcinoma cells using laser tweezer Raman spectroscopy

Qiu¹,

Li²,

Li³

et al. 2020

Biomed. Opt. Express

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To explore the effect in nasopharyngeal carcinoma (NPC) cells after treatment with chemodrugs, Raman profiles were characterized by laser tweezer Raman spectroscopy. Two NPC cell lines (CNE2 and C666-1) were treated with gemcitabine, cisplatin, and paclitaxel, respectively. The high-quality Raman spectra of cells without or with treatments were recorded at the single-cell level with label-free laser tweezers Raman spectroscopy (LTRS) and analyzed for the differences of alterations of Raman profiles. Tentative assignments of Raman peaks indicated that the cellular specific biomolecular changes associated with drug treatment include changes in protein structure (e.g. 1655 cm −1), changes in DNA/RNA content and structure (e.g. 830 cm −1), destruction of DNA/RNA base pairs (e.g. 785 cm −1), and reduction in lipids (e.g. 970 cm −1). Besides, both principal components analysis (PCA) combined with linear discriminant analysis (LDA) and the classification and regression trees (CRT) algorithms were employed to further analyze and classify the spectral data between control group and treated group, with the best discriminant accuracy of 96.7% and 90.0% for CNE2 and C666-1 group treated with paclitaxel, respectively. This exploratory work demonstrated that LTRS technology combined with multivariate statistical analysis has promising potential to be a novel analytical strategy at the single-cell level for the evaluation of NPC-related chemotherapeutic drugs.

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“…While this work shows application to tissue homogenates, DAPNe has been applied to extract single cells and even their organelles from their local environment, through pneumatically pulling analytes through a capillary tip. Future work should explore whether DAPNe-LC-MS has the sensitivity to operate effectively at these reduced sampling dimensions and whether comparable precision can be obtained at these lower sampling volumes.…”

Section: Resultsmentioning

confidence: 99%

“…Due to the smaller (1 μm) tip diameter, DAPNe has the ability to gain superior spatial resolution, with a 6 μm spot diameter having been previously reported . Many potential applications have been demonstrated in forensics − and biosciences − with the tool having sufficient resolution and flexibility to probe individual cells and their organelles …”

Section: Introductionmentioning

confidence: 99%

Nanoextraction Coupled to Liquid Chromatography Mass Spectrometry Delivers Improved Spatially Resolved Analysis

et al. 2019

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Direct analyte-probed nanoextraction (DAPNe) is a technique that allows extraction of drug and endogenous compounds from a discrete location on a tissue sample using a nano capillary filled with solvent. Samples can be extracted from spot diameters as low as 6 μm. Studies previously undertaken by our group have shown that the technique can provide good precision (5%) for analyzing drug molecules in 150 μm diameter areas of homogenized tissue, provided an internal standard is sprayed on to the tissue prior to analysis. However, without an isotopically labeled standard, the repeatability is poor, even after normalization to the spot area or matrix compounds. By application to tissue homogenates spiked with drug compounds, we can demonstrate that it is possible to significantly improve the repeatability of the technique by incorporating a liquid chromatography separation step. Liquid chromatography is a technique for separating compounds prior to mass spectrometry (LC-MS) which enables separation of isomeric compounds that cannot be discriminated using mass spectrometry alone, as well as reducing matrix interferences. Conventionally, LC-MS is carried out on bulk or homogenized samples, which means analysis is essentially an average of the sample and does not take into account discrete areas. This work opens a new opportunity for spatially resolved liquid chromatography mass spectrometry with precision better than 20%.

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True one cell chemical analysis: a review

Abstract: The constantly growing field of True One Cell (TOC) analysis has provided important information on the direct chemical composition of various cells and cellular components.

Cited by 11 publications

References 124 publications

Nanocapillary sampling coupled to liquid chromatography mass spectrometry delivers single cell drug measurement and lipid fingerprints

Nanocapillary sampling coupled to liquid chromatography mass spectrometry delivers single cell drug measurement and lipid fingerprints

Study on the chemodrug-induced effect in nasopharyngeal carcinoma cells using laser tweezer Raman spectroscopy

Nanoextraction Coupled to Liquid Chromatography Mass Spectrometry Delivers Improved Spatially Resolved Analysis

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