True histiocytic neoplasm of Langerhans' cell type

Delabie, Jan; Wolf‐Peeters, C. De; Vos, Rita De; Vandenberghe, Elisabeth; Kennes, K.; Jonge, Inge De; Desmet, Valeer

doi:10.1002/path.1711630306

Cited by 41 publications

(29 citation statements)

References 17 publications

(1 reference statement)

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“…4,32,35 The present double immunostaining of lymph nodes failed to demonstrate that anti-S-100 protein antibody-positive interdigitating cells correspond to AM-3K-positive cells. Our findings that no cells were reacting to AM-3K in the epidermis suggest negative reactions of Langerhans cells to AM-3K.…”

Section: Discussionmentioning

confidence: 62%

“…An antibody against S-100 protein labels dendritic cells in lymph nodes as well as neural tissues such as neural, glial, and Schwann cells. 4,35 The double immunocytochemistry with AM-3K and rabbit anti-cow S-100 protein antibody (undiluted; DAKO) was conducted on lymph nodes obtained from dogs and cattle. After the immunostaining with anti-S-100 protein antibody by ABC methods, the immunostaining with AM-3K was carried out using the alkaline phosphatase antialkaline phosphatase (APAAP Kit, DAKO) methods.…”

Section: Immunocytochemistrymentioning

confidence: 99%

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Distribution of Cells Immunopositive for AM-3K, a Novel Monoclonal Antibody Recognizing Human Macrophages, in Normal and Diseased Tissues of Dogs, Cats, Horses, Cattle, Pigs, and Rabbits

et al. 2000

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Abstract. The monoclonal antibody AM-3K, which was developed using human pulmonary macrophages as the immunogen, immunocytochemically labels most human macrophages except for blood monocytes and dendritic cell populations. AM-3K also shows cross-reactivity in some animal species. To evaluate the usefulness of AM-3K, the present study investigated the detailed distribution of AM-3K-immunopositive macrophages in normal and diseased tissues of dogs, cats, horses, cattle, pigs, and rabbits. Zamboni's solution-fixed, paraffinembedded sections were the most available for the immunocytochemistry with AM-3K. In all animal species examined, AM-3K labeled most macrophages in splenic red pulp, lymph node sinuses and thymus, and tissue macrophages in the interstitium of various organs and sites such as the kidneys, lungs, heart, pancreas, intestines, and skin. Alveolar macrophages and perivascular microglial cells were also immunoreactive for AM-3K. Interestingly, Kupffer cells of dogs, cats, and horses were labeled for AM-3K, but those of cattle, pigs, and rabbits were not. Furthermore, in tumor tissues and inflammatory lesions such as liver fibrosis and encephalomalacia that were obtained from dogs, infiltrating macrophages were stained with AM-3K, but not all infiltrating macrophages reacted to AM-3K. In addition, only 30-50% of pulmonary and peritoneal macrophages obtained from cats and dogs were reactive for AM-3K. AM-3K did not react with blood monocytes, dendritic cell populations, and osteoclasts. These observations indicate that AM-3K specifically labels most exudate and tissue macrophages in the animal species examined. However, the expression of antigens recognized by AM-3K on macrophages may be dependent on differential maturation stages or different functions evoked by some conditions. AM-3K immunoreaction products were seen on the cytoplasmic membrane of macrophages by immunoelectron microscopy. AM-3K would be useful for detection of macrophage populations in the animal species examined here.

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Section: Discussionmentioning

confidence: 62%

Section: Immunocytochemistrymentioning

confidence: 99%

Distribution of Cells Immunopositive for AM-3K, a Novel Monoclonal Antibody Recognizing Human Macrophages, in Normal and Diseased Tissues of Dogs, Cats, Horses, Cattle, Pigs, and Rabbits

et al. 2000

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“…Until the current day, a total of 15 cases considered as LCS, including the present case, have been reported and reviewed in medical literature [2,3,6,8,9,11,13]. In these few cases, a wide diversity concerning age, site of occurrence, progression and applied treatments has been observed.…”

Section: Discussionmentioning

confidence: 95%

Pulmonary manifestation of a Langerhans cell sarcoma: case report and review of the literature

2005

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In the following, we describe the very rare case of Langerhans cell sarcoma (LCS) in the lung. Throughout the medical literature, only a few cases have been published, and, to the best of our knowledge, this is the first case to be reported in Germany. The patient was an 81-year-old man who showed symptoms such as chronic cough and weight loss. Clinical examination including needle biopsy indicated a high possibility of carcinoma in the right lung and in the mediastinum; however, the final histopathological diagnosis after immunohistochemistry gave evidence of LCS. LCS is a neoplastic proliferation of Langerhans cells with malignant cytological features exhibiting a very aggressive behaviour. LCS can be distinguished from other carcinomas, lymphomas and sarcomas by the typical morphological features of Langerhans cells and the immunophenotype with a consistent expression of S-100 protein and CD1a. In contrast to Langerhans cell histiocytosis, the LCS consists of Langerhans cells with high atypia and a very high mitotic rate.

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“…ED2 reacted with cell membrane antigens of rat resident macrophages (Kupffer cells in the liver) [16,46]. ACP and NSE are lysosomal enzymes that have been used as markers for histiocytic sarcomas [13,22]. Positive reactions to ACP and NSE were seen in KB tumor cells, KB-P and KB-D8 cells, and cells constituting tumors induced by KB-P and KB-D8.…”

Section: Discussionmentioning

confidence: 99%

“…q For TGF-β1, 24 cycles of 1 min denaturation at 94°C, 1 min annealing at 62°C, and 1 min synthesis at 72°C [42] q For TNF-α, 27 cycles of 1 min denaturation at 94°C, 1 min annealing at 57°C, and 1 min synthesis at 72°C [13] The oligonucleotides used were as follows: the TGF-β1 sense primer 5′-CTTCAGCTCCACAGAGAAGAACTGC-3′ and antisense primer 5′-CACGATCATGTTGGACAACTGCTCC-3′ [42]; the TNF-α sense primer 5′-TGTCTACTGAACTTCGGGGTG-3′ and antisense primer 5′-GAGGCTGACTTTCTCCTGGTA-3′ [14]; the β-actin sense primer 5′-TAAAGACCTCTATGCCAA-CAC-3′ and antisense primer 5′-CTCCTGCTTGCTGATCCA-CAT-3′ [43,52]. The PCR products were subjected to electrophoresis in agarose gel.…”

Section: Methodsmentioning

confidence: 99%

Establishment and characterization of transplantable tumor line (KB) and cell lines (KB-P and KB-D8) from a rat thymus-derived dendritic cell sarcoma

Yamate

Kawashima

et al. 2002

Virchows Arch

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A transplantable tumor line (KB) was established in syngeneic rats from a naturally occurring sarcoma that had arisen in the thymus of a 24-month-old male F344 rat. Further, a cell line (KB-P) was induced from KB and a cloned cell line (KB-D8) was isolated from KB-P. The primary thymic tumor and KB tumors showed heterogeneous histological growth patterns such as sheet-like, ill-defined bundle, fascicular and interwoven fashions, consisting of spindle cells, oval cells and histiocytic large round cells. Immunohistochemically, neoplastic cells in KB tumors and KB-P and KB-D8 cultures reacted to vimentin and were labeled with antibodies of OX6 (for rat major histocompatibility complex class-II antigens), ED5 (for rat follicular dendritic cells; FDCs) and RED-1 (for interdigitating dendritic cells) in varying degrees, indicating that neoplastic cells exhibited the immunophenotypes of rat dendritic cells. In addition, neoplastic cells were immunoreactive to ED1 (for rat monocytes/macrophages) and ED2 (for rat tissue macrophages), and also showed positive reactions to histiocytic lysosomal enzymes such as acid phosphatase and non-specific esterase. Ultrastructurally, neoplastic cells had cell surface projections, cisterna-like structures and variously developed lysosomes in the cytoplasm. Based on these findings, the present tumor was regarded as dendritic cell-derived sarcoma capable of expressing macrophage-like and histiocytic nature. A reverse-transcription polymerase chain reaction method revealed that the addition of lipopolysaccharide dose dependently increased the expression of mRNA of transforming growth factor-beta1, a proinflammatory factor, in KB-D8 cells. The transplantable line (KB) and cell lines (KB-P and KB-D8) may become useful tools for studying the histogenesis and pathobiological functions of dendritic cells.

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True histiocytic neoplasm of Langerhans' cell type

Cited by 41 publications

References 17 publications

Distribution of Cells Immunopositive for AM-3K, a Novel Monoclonal Antibody Recognizing Human Macrophages, in Normal and Diseased Tissues of Dogs, Cats, Horses, Cattle, Pigs, and Rabbits

Distribution of Cells Immunopositive for AM-3K, a Novel Monoclonal Antibody Recognizing Human Macrophages, in Normal and Diseased Tissues of Dogs, Cats, Horses, Cattle, Pigs, and Rabbits

Pulmonary manifestation of a Langerhans cell sarcoma: case report and review of the literature

Establishment and characterization of transplantable tumor line (KB) and cell lines (KB-P and KB-D8) from a rat thymus-derived dendritic cell sarcoma

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