TRRAP and GCN5 are used by c-Myc to activate RNA polymerase III transcription

Kenneth, Niall S.; Ramsbottom, Ben A.; Gomez-Roman, Natividad; Marshall, Lynne; Cole, Philip A.; White, Robert J.

doi:10.1073/pnas.0702909104

Cited by 104 publications

(107 citation statements)

References 50 publications

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“…2). Consistent with the report by another group (19), endogenous c-Myc readily bound to the rDNA genes as well as the 5 S rRNA, tRNA Leu , and tRNA Tyr genes (Fig. 2).…”

Section: Ablation Of Endogenous L11 Enhances C-myc-dependentsupporting

confidence: 91%

“…For instance, similar to the case of c-Myc-induced transcription by RNA Pol I and II, the TRRAP co-activator is also required for c-Myc-mediated transcription by RNA Pol III of the 5 S rRNA and tRNA genes, and c-Myc can bind to these genes and promote the association of TRRAP with them, although no typical E-box elements specific for c-Myc-binding were identified in the promoters of these genes (19). Because the 5 S rRNA and tRNA genes are essential for ribosomal biogenesis and translation, the expression of these genes must also be tightly regulated for normal cell growth.…”

mentioning

confidence: 83%

“…Transcription of 5 S rRNA and tRNA Genes-It has been shown that c-Myc directly binds to 5 S rRNA and tRNA genes and enhances their transcription catalyzed by RNA Pol III (16,19). …”

Section: Ablation Of Endogenous L11 Enhances C-myc-dependentmentioning

confidence: 99%

“…L11 Binds to the 5 S rRNA and tRNA Genes-Unlike the rDNA genes, which harbor a number of E-box elements, the 5 S rRNA and tRNA gene promoters do not usually contain typical E-box elements (19). However, c-Myc does bind to these genes, and the binding is important for their c-Myc-dependent expression (16,19).…”

Section: Ablation Of Endogenous L11 Enhances C-myc-dependentmentioning

confidence: 99%

“…However, c-Myc does bind to these genes, and the binding is important for their c-Myc-dependent expression (16,19). To elucidate how L11 regulates c-Myc-mediated transcription of these genes, we next tested if L11 associates with c-Myc at these genes using ChIP-qPCR assays because we previously showed that L11 physically associates with c-Myc at the promoters of several RNA Pol I-and II-transcribed genes (18).…”

Section: Ablation Of Endogenous L11 Enhances C-myc-dependentmentioning

confidence: 99%

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Ribosomal Protein L11 Associates with c-Myc at 5 S rRNA and tRNA Genes and Regulates Their Expression

Dai

Sun

2010

Journal of Biological Chemistry

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The c-Myc oncoprotein promotes cell growth by enhancing ribosomal biogenesis. Overexpression of c-Myc and aberrant ribosomal biogenesis lead to deregulated cell growth and tumorigenesis. Hence, c-Myc activity and ribosomal biogenesis must be tightly coordinated during normal homeostasis. We previously found that ribosomal protein L11 inhibits c-Myc activity by blocking the recruitment of its co-activator transformation/transcription domain-associated protein (TRRAP) to the promoter regions of c-Myc target genes that are transcribed by RNA polymerases I and II. In this study, we extended the role of L11 to the regulation of c-Myc-driven transcription of the 5 S rRNA and tRNA genes by RNA polymerase III. L11 co-resided with c-Myc at the 5 S rRNA and tRNA genes and significantly inhibited the binding of TRRAP to these genes. Knocking down endogenous L11 enhanced c-Myc-dependent transcription of these genes. Interestingly, in response to ribosomal stress induced by the treatment of cells with a low dose of actinomycin D or serum starvation, L11 binding to these genes was increased, and inversely TRRAP binding to these genes was decreased. Consistently, knockdown of L11 rescued the reduction of the expression of these genes by the two treatments. These results demonstrate that L11 suppresses c-Myc-dependent and RNA polymerase III-catalyzed transcription of 5 S rRNA and tRNA genes in response to ribosomal stress, ensuring a tight coordination between c-Myc activity and ribosomal biogenesis.

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“…2). Consistent with the report by another group (19), endogenous c-Myc readily bound to the rDNA genes as well as the 5 S rRNA, tRNA Leu , and tRNA Tyr genes (Fig. 2).…”

Section: Ablation Of Endogenous L11 Enhances C-myc-dependentsupporting

confidence: 91%

mentioning

confidence: 83%

“…Transcription of 5 S rRNA and tRNA Genes-It has been shown that c-Myc directly binds to 5 S rRNA and tRNA genes and enhances their transcription catalyzed by RNA Pol III (16,19). …”

Section: Ablation Of Endogenous L11 Enhances C-myc-dependentmentioning

confidence: 99%

Section: Ablation Of Endogenous L11 Enhances C-myc-dependentmentioning

confidence: 99%

Section: Ablation Of Endogenous L11 Enhances C-myc-dependentmentioning

confidence: 99%

See 3 more Smart Citations

Ribosomal Protein L11 Associates with c-Myc at 5 S rRNA and tRNA Genes and Regulates Their Expression

Dai

Sun

2010

Journal of Biological Chemistry

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Targeting MYC in cancer therapy: RNA processing offers new opportunities

2016

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MYC is a transcription factor, which not only directly modulates multiple aspects of transcription and co‐transcriptional processing (e.g. RNA‐Polymerase II initiation, elongation, and mRNA capping), but also indirectly influences several steps of RNA metabolism, including both constitutive and alternative splicing, mRNA stability, and translation efficiency. As MYC is an oncoprotein whose expression is deregulated in multiple human cancers, identifying its critical downstream activities in tumors is of key importance for designing effective therapeutic strategies. With this knowledge and recent technological advances, we now have multiple angles to reach the goal of targeting MYC in tumors, ranging from the direct reduction of MYC levels, to the dampening of selected house‐keeping functions in MYC‐overexpressing cells, to more targeted approaches based on MYC‐induced secondary effects.

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RNA Polymerase III Transcription – Regulated by Chromatin Structure and Regulator of Nuclear Chromatin Organization

Pascali

Teichmann

2012

Subcellular Biochemistry

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RNA polymerase III (Pol III) transcription is regulated by modifications of the chromatin. DNA methylation and post-translational modifications of histones, such as acetylation, phosphorylation and methylation have been linked to Pol III transcriptional activity. In addition to being regulated by modifications of DNA and histones, Pol III genes and its transcription factors have been implicated in the organization of nuclear chromatin in several organisms. In yeast, the ability of the Pol III transcription system to contribute to nuclear organization seems to be dependent on direct interactions of Pol III genes and/or its transcription factors TFIIIC and TFIIIB with the structural maintenance of chromatin (SMC) protein-containing complexes cohesin and condensin. In human cells, Pol III genes and transcription factors have also been shown to colocalize with cohesin and the transcription regulator and genome organizer CCCTC-binding factor (CTCF). Furthermore, chromosomal sites have been identified in yeast and humans that are bound by partial Pol III machineries (extra TFIIIC sites - ETC; chromosome organizing clamps - COC). These ETCs/COC as well as Pol III genes possess the ability to act as boundary elements that restrict spreading of heterochromatin.

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TRRAP and GCN5 are used by c-Myc to activate RNA polymerase III transcription

Cited by 104 publications

References 50 publications

Ribosomal Protein L11 Associates with c-Myc at 5 S rRNA and tRNA Genes and Regulates Their Expression

Ribosomal Protein L11 Associates with c-Myc at 5 S rRNA and tRNA Genes and Regulates Their Expression

Targeting MYC in cancer therapy: RNA processing offers new opportunities

RNA Polymerase III Transcription – Regulated by Chromatin Structure and Regulator of Nuclear Chromatin Organization

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