TRPV1 receptor inhibition decreases CCL2-induced hyperalgesia

Spicarova, Diana; Adámek, Pavel; Kalynovska, Nataliia; Mrózková, Petra; Paleček, J.

doi:10.1016/j.neuropharm.2014.01.041

Cited by 41 publications

(37 citation statements)

References 46 publications

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“…Under naive conditions, there was a tendency for the antagonist of CB 1 receptors, per se, to non‐significantly increase the frequency of sEPSC, whereas the TRPV1 channel antagonist did not affect the superficial dorsal horn neurons sEPSC frequency, as previously reported (Spicarova et al, ). Nevertheless, moderate TRPV1 channel‐mediated sEPSC tonic activity was reported in lamina II neurons in mice (Park et al, ).…”

Section: Discussionsupporting

confidence: 80%

“…20:4‐NAPE (Avanti Polar Lipids) was dissolved in chloroform, which had a concentration of < 0.1% in the final solution. The concentration of the selective TRPV1 channel antagonist SB366791 (10 μM) used here was based on our previous studies (Spicarova et al, ; Spicarova and Palecek, ) and its selectivity pA 2 = 7.71 (Gunthorpe et al, ). The concentration of the highly selective CB 1 receptor antagonist PF514273 (0.2 μM) was determined by considering Ki ‐ 1 nM (Dow et al, ) and the needed diffusion through the spinal cord slice.…”

Section: Methodsmentioning

confidence: 99%

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Peripheral inflammation affects modulation of nociceptive synaptic transmission in the spinal cord induced by N‐arachidonoylphosphatidylethanolamine

Nerandžič

Mrózková

Adámek

et al. 2017

British J Pharmacology

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Section: Discussionsupporting

confidence: 80%

Section: Methodsmentioning

confidence: 99%

Peripheral inflammation affects modulation of nociceptive synaptic transmission in the spinal cord induced by N‐arachidonoylphosphatidylethanolamine

Nerandžič

Mrózková

Adámek

et al. 2017

British J Pharmacology

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“…Synovial fluid CCL2 has been associated with OA knee pain severity, in addition to physical disability 39 . During inflammation, elevated expression of CCL2 might act on sensory nerves to activate transient receptor potential cation channel subfamily V member 1 (TRPV1) to induce hyperalgesia 40 . CCL8 has previously been detected in fibroblasts and macrophages in the synovial lining of arthritic patients 35 .…”

Section: Discussionmentioning

confidence: 99%

Molecular expression patterns in the synovium and their association with advanced symptomatic knee osteoarthritis

Nwosu

Wilson

Hill

et al. 2019

Osteoarthritis and Cartilage

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s u m m a r yObjective: Osteoarthritis (OA) is a major source of knee pain. Mechanisms of OA knee pain are incompletely understood but include synovial pathology. We aimed to identify molecular expression patterns in the synovium associated with symptomatic knee OA. Design: Snap frozen synovia were from people undergoing total knee replacement (TKR) for advanced OA, or from post-mortem (PM) cases who had not sought help for knee pain. Associations with OA symptoms were determined using discovery and validation samples, each comprising TKR and post mortem (PM) cases matched for chondropathy (Symptomatic or Asymptomatic Chondropathy). Associations with OA were determined by comparing age matched TKR and PM control cases. Real-time quantitative PCR for 96 genes involved in inflammation and nerve sensitisation used TaqMan ® Array Cards in discovery and validation samples, and protein expression for replicated genes was quantified using Luminex bead assay. Results: Eight genes were differentially expressed between asymptomatic and symptomatic chondropathy cases and replicated between discovery and validation samples (P<0.05 or >3-fold change). Of these, matrix metalloprotease (MMP)-1 was also increased whereas interleukin-1 receptor 1 (IL1R1) and vascular endothelial growth factor (VEGF) were decreased at the protein level in the synovium of symptomatic compared to asymptomatic chondropathy cases. MMP1 protein expression was also increased in OA compared to PM controls. Conclusion: Associations of symptomatic OA may suggest roles of MMP1 expression and IL1R1 and VEGF pathways in OA pain. Better understanding of which inflammation-associated molecules mediate OA pain should inform refinement of existing therapies and development of new treatments.

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“…Different from these results, increased expression of MCP-1 was reported in astrocytes after nerve injury (Gao et al, 2009). It was also shown that TRPV1 was implicated in CCL2-induced excitatory postsynaptic currents (Spicarova et al, 2014).…”

Section: Introductionmentioning

confidence: 86%

Monocyte chemoattractant protein-1-induced excitation and sensitization to mechanical stimulation of mechanosensitive C-fiber afferents in rat skin

Kubo

Taguchi

Mizumura

2015

Neuroscience Research

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TRPV1 receptor inhibition decreases CCL2-induced hyperalgesia

Cited by 41 publications

References 46 publications

Peripheral inflammation affects modulation of nociceptive synaptic transmission in the spinal cord induced by N‐arachidonoylphosphatidylethanolamine

Peripheral inflammation affects modulation of nociceptive synaptic transmission in the spinal cord induced by N‐arachidonoylphosphatidylethanolamine

Molecular expression patterns in the synovium and their association with advanced symptomatic knee osteoarthritis

Monocyte chemoattractant protein-1-induced excitation and sensitization to mechanical stimulation of mechanosensitive C-fiber afferents in rat skin

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