TRPM8 Regulates Sexual Desire and Satiety

Abstract

show here that human muscarinic acetylcholine receptor 1 (hM1R) activation by carbachol inhibits TRPM8 in a mammalian expression system. We found that intracellular dialysis of PIP 2 alleviated the inhibition, and PIP 2 was depleted in the plasma membrane when hM1R was activated. We also found that TRPM8 channels are partially inhibited by a constitutively active Gaq when PLC is not activated, confirming earlier results. Furthermore, we used an ''inflammatory soup'', which contains multiple agonists of various Gaq-protein coupled receptors expressed in dorsal root ganglion (DRG) neurons, to test our hypothesis in neurons. We found that intracellular dialysis of PIP 2 alleviates the inhibition of TRPM8 by Gaq-protein coupled receptors in DRG neurons. We conclude that a decrease in PIP 2 level contributes to inhibition of TRPM8 channels upon the activation of Gaq-protein coupled receptors.

Expression of TRPM8 in the prostate of rams and wethers

“…Prostate carcinoma cells are sensitive to androgen concentrations and require high androgen concentrations for growth and cell survival ( Kulkarni, 2009 ; Liu et al, 2016 ). Demirkhanyan et al, 2018 hypothesized that TRPM8 channels might directly influence sexual behavior of animals since these channels are influenced by testosterone dependent signaling ( Demirkhanyan et al, 2018 ). From the results presented in this paper TRPM8 expression may influence functionality and efficiency of the male reproductive tract.…”