Trpm7 Protein Contributes to Intercellular Junction Formation in Mouse Urothelium

Abstract

Background: Transient receptor potential melastatin 7 (Trpm7) is a Ca 2ϩ -permeable channel with a kinase domain that is implicated in cell migration and adhesion. Results: Urothelium-specific Trpm7 knock-out caused immature intercellular junctions, inflammation, and smaller voided volume. Conclusion: Trpm7 contributes to the intercellular junction formation in the urothelium. Significance: These findings might provide the first evidence for the significance of Trpm7 in bladder function in vivo.

Development of novel and non‐invasive diagnostic markers for lower urinary tract symptoms using urothelial cells in voided urine

“…21 Another study collecting mouse urothelial cells suggested that down-regulated TRPM7 causes immature intercellular junctions, inflammation, and smaller voided volume. 3 In the present study, TRPM7 was negatively correlated with pollakisuria only in IC patients. We speculated from our result that the barrier function could be disrupted in IC, inducing inflammation and pollakisuria.…”

“…TRPM7 is functionally expressed in mouse urothelial cells, and is thought to act as a mechano‐sensor and/or chemo‐sensor . Another study collecting mouse urothelial cells suggested that down‐regulated TRPM7 causes immature intercellular junctions, inflammation, and smaller voided volume . In the present study, TRPM7 was negatively correlated with pollakisuria only in IC patients.…”

“…Despite many studies of these channels’ discovery, it may take quite a few years to develop new drugs as the target for these channels . On the other hand, when investigating the pathophysiology of LUTS and to diagnose LUTS refractory to treatments, these channels on the urothelium may be useful, because animal studies already have revealed that the expression of Cx and TRP channels on urothelial cells are associated with LUTS . However, evidence of these associations remains unclear in humans.…”

<b>ATP release from bladder urothelium and serosa in a rat model of partial bladder outlet </b><b>obstruction </b>

“…Little is known about the mechanisms about bladder activity. Several ion channels such as TRPM7 (30) or PIEZO1 (26) have also been known to be involved in bladder activity. However, pBOO did result in an increase in bladder weight (Fig.…”

Roles of transient receptor potential channels in regulation of vascular and epithelial barriers

“…70 TRPM7 has been implicated in the formation of tight junctions in the mouse urinary bladder epithelium. 71 Bladders of inducible urothelium-specific TRPM7 knockout mice displayed submucosal edema and shorter length of tight junctions along the urothelium. Elevated levels of Interleukin-1b and Tumor Necrosis Factor a were also detected in the bladder wall.…”