TRPM7 is involved in angiotensin II induced cardiac fibrosis development by mediating calcium and magnesium influx

Transient Receptor Potential Melastatin 7 (TRPM7) Contributes to H2O2-Induced Cardiac Fibrosis via Mediating Ca2+ Influx and Extracellular Signal–Regulated Kinase 1/2 (ERK1/2) Activation in Cardiac Fibroblasts

“…One of our previous studies has shown that nifedipine and verapamil, classical blockers of L-type calcium channels, had no effect on angiotensin II-induced elevation of [Ca 2+ ] i in cardiac fibroblasts (33), indicating some channels other than voltage-dependent calcium channels may contribute to the increase of [Ca 2+ ] i in cardiac fibroblasts and resultant fibrosis. Furthermore, our recent studies have demonstrated that TRPM7 is involved in angiotensin II-induced cardiac fibrosis by mediating calcium influx in cardiac fibroblasts (34). These findings indicate that TRPM7-mediated Ca 2+ influx plays a crucial role in myocardial fibrogenesis.…”

SLC41A1 knockdown inhibits angiotensin II-induced cardiac fibrosis by preventing Mg2+ efflux and Ca2+ signaling in cardiac fibroblasts

“…Firstly, previous studies have shown that TRPM7 vastly accelerated cardiac fibrosis responding to pathological stimulus through Ca 2+ influx [13,45]. And it has been reported that TRPM7 is negatively regulated by intracellular levels of Mg 2+ and Mg-ATP [46], which means that the increased [Mg 2+ ] i is sufficient to inhibit TRPM7 channels.…”

“…of cardiac fibroblasts [12]. The early studies in our laboratory have found that stimulation of Ang II in cardiac fibroblasts elicits an extrusion of cellular Mg 2+ [13]. And Ang II is known to activate the Na + /Mg 2+ exchanger in vascular and renal cells to expedite the development of hypertension through enhancing vascular reactivity, vascular remodeling and/or impairing vasodilation [14].…”

Functional Alterations of Ion Channels From Cardiac Fibroblasts in Heart Diseases

“…22 TRPM7 protein expression increases by 24 hours in the presence of angiotensin II. 44 Silencing TRPM7 channels by short hairpin RNA interference reduced hydrogen peroxide-induced activation of Ca 2+ influx and 2-aminoethoxydiphenylborate, a TRPM7 blocker that could inhibit hydrogen peroxide-induced cardiac fibrosis. 28 In addition to Ca 2+ , another permeant ion, Mg 2+ --an essential cofactor in numerous enzymatic reactions via Mg 2+ -dependent regulation of MAPK, tyrosine kinases, reactive oxygen species and important signaling molecules --is involved in cell proliferation, differentiation, inflammation, etc.…”