TRPM5, a taste-signaling transient receptor potential ion-channel, is a ubiquitous signaling component in chemosensory cells

Kaske, Silke; Krasteva, Gabriela; König, Peter; Kummer, Wolfgang; Hofmann, Thomas; Gudermann, Thomas; Chubanov, Vladimir

doi:10.1186/1471-2202-8-49

Cited by 202 publications

(205 citation statements)

References 69 publications

(89 reference statements)

Supporting

Mentioning

195

Contrasting

Unclassified

Order By: Relevance

“…S4, available at www.jneurosci.org as supplemental material). In summary, the results from these experiments indicate that these salts, should they diffuse or be transported to the basolateral side of taste cells where TRPM5 is located (Kaske et al, 2007), are unlikely to directly activate TRPM5. In this regard, direct activation of the TRPM5 channel is unlikely to account for the behavioral changes seen with these compounds.…”

Section: Effects Of Ctds On Heterologously Expressed Htrpm5 Channelsmentioning

confidence: 95%

Sensory Attributes of Complex Tasting Divalent Salts Are Mediated by TRPM5 and TRPV1 Channels

Riera¹,

Vogel²,

Simon³

et al. 2009

J. Neurosci.

View full text Add to dashboard Cite

Complex tasting divalent salts (CTDS) are present in our daily diet, contributing to multiple poorly understood taste sensations. CTDS evoking metallic, bitter, salty, and astringent sensations include the divalent salts of iron, zinc, copper, and magnesium. To identify pathways involved with the complex perception of the above salts, taste preference tests (two bottles, brief access) were performed in wild-type (WT) mice and in mice lacking (1) the T1R3 receptor, (2) TRPV1, the capsaicin receptor, or (3) the TRPM5 channel, the latter being necessary for the perception of sweet, bitter, and umami tasting stimuli. At low concentrations, FeSO 4 and ZnSO 4 were perceived as pleasant stimuli by WT mice, and this effect was fully reversed in TRPM5 knock-out mice. In contrast, MgSO 4 and CuSO 4 were aversive to WT mice, but for MgSO 4 the aversion was abolished in TRPM5 knock-out animals, and for CuSO 4 , aversion decreased in both TRPV1-and TRPM5-deficient animals. Behavioral tests revealed that the T1R3 subunit of the sweet and umami receptors is implicated in the hedonically positive perception of FeSO 4 and ZnSO 4 . For high concentrations of CTDS, the omission of TRPV1 reduced aversion. Imaging studies on heterologously expressed TRPM5 and TRPV1 channels are consistent with the behavioral experiments. Together, these results rationalize the complexity of metallic taste by showing that at low concentrations, compounds such as FeSO 4 and ZnSO 4 stimulate the gustatory system through the hedonically positive T1R3-TRPM5 pathway, and at higher concentrations, their aversion is mediated, in part, by the activation of TRPV1.

show abstract

Section: Effects Of Ctds On Heterologously Expressed Htrpm5 Channelsmentioning

confidence: 95%

Sensory Attributes of Complex Tasting Divalent Salts Are Mediated by TRPM5 and TRPV1 Channels

Riera¹,

Vogel²,

Simon³

et al. 2009

J. Neurosci.

View full text Add to dashboard Cite

show abstract

“…Although T2Rs and their related downstream signaling elements were first identified in taste cells (30,31), the T2R receptor cascade is present in numerous cells throughout the body, including the gastrointestinal tract (3,32) and the respiratory tree (1, 3, 4, 10, 33, 34). Most of these reports describe a limited population of specialized epithelial cells similar in morphology and molecular signature to the solitary chemosensory cells that we describe.…”

Section: Discussionmentioning

confidence: 99%

“…1), which respond to a wide variety of irritants to activate trigeminal nerve-mediated protective airway reflexes such as sneezing, coughing, or apnea (5) as well as local neurogenic inflammatory responses (6,7). The SCCs express many elements of the bitter taste signaling pathway, including the Tas2R family of receptors, Gα-gustducin, phospholipase Cβ2 (PLCβ2), and the monovalent-selective cation channel TrpM5 (1,3,4). Receptorligand interaction results in a transduction cascade involving activation of Gα-gustducin, PLC-mediated release of Ca 2+ from intracellular stores, Ca 2+ -dependent activation of TrpM5, membrane depolarization, and, presumably, neurotransmitter release and subsequent excitation of capsaicin-sensitive trigeminal pain fibers that richly innervate the chemosensory cells ( Fig.…”

mentioning

confidence: 99%

Nasal chemosensory cells use bitter taste signaling to detect irritants and bacterial signals

Tizzano

Gulbransen

Vandenbeuch

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

350

436

View full text Add to dashboard Cite

The upper respiratory tract is continually assaulted with harmful dusts and xenobiotics carried on the incoming airstream. Detection of such irritants by the trigeminal nerve evokes protective reflexes, including sneezing, apnea, and local neurogenic inflammation of the mucosa. Although free intra-epithelial nerve endings can detect certain lipophilic irritants (e.g., mints, ammonia), the epithelium also houses a population of trigeminally innervated solitary chemosensory cells (SCCs) that express T2R bitter taste receptors along with their downstream signaling components. These SCCs have been postulated to enhance the chemoresponsive capabilities of the trigeminal irritant-detection system. Here we show that transduction by the intranasal solitary chemosensory cells is necessary to evoke trigeminally mediated reflex reactions to some irritants including acyl–homoserine lactone bacterial quorum-sensing molecules, which activate the downstream signaling effectors associated with bitter taste transduction. Isolated nasal chemosensory cells respond to the classic bitter ligand denatonium as well as to the bacterial signals by increasing intracellular Ca 2 + . Furthermore, these same substances evoke changes in respiration indicative of trigeminal activation. Genetic ablation of either Gα-gustducin or TrpM5, essential elements of the T2R transduction cascade, eliminates the trigeminal response. Because acyl–homoserine lactones serve as quorum-sensing molecules for Gram-negative pathogenic bacteria, detection of these substances by airway chemoreceptors offers a means by which the airway epithelium may trigger an epithelial inflammatory response before the bacteria reach population densities capable of forming destructive biofilms.

show abstract

“…Respiratory chemosensory brush cells express elements of the canonical taste transduction cascade, including the taste-specific G protein α-gustducin, phospholipase C β2 (PLCβ2), and the transient potential receptor cation channel melanostatin 5 (TRPM5) (5,6,(15)(16)(17). These proteins are also expressed in urethras from ChAT-eGFP and WT mice (Fig.…”

Section: Cholinergic Urethral Brush Cells Use the Canonical Taste Tramentioning

confidence: 99%

Bitter triggers acetylcholine release from polymodal urethral chemosensory cells and bladder reflexes

Deckmann

Filipski

Krasteva‐Christ

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

141

213

View full text Add to dashboard Cite

Significance We report the presence of a previously unidentified cholinergic, polymodal chemosensory cell in the mammalian urethra, the potential portal of entry for bacteria and harmful substances into the urogenital system. These cells exhibit structural markers of respiratory chemosensory cells (“brush cells”). They use the classical taste transduction cascade to detect potential hazardous compounds (bitter, umami, uropathogenic bacteria) and release acetylcholine in response. They lie next to sensory nerve fibers that carry acetylcholine receptors, and placing a bitter compound in the urethra enhances activity of the bladder detrusor muscle. Thus, monitoring of urethral content is linked to bladder control via a previously unrecognized cell type.

show abstract

TRPM5, a taste-signaling transient receptor potential ion-channel, is a ubiquitous signaling component in chemosensory cells

Cited by 202 publications

References 69 publications

Sensory Attributes of Complex Tasting Divalent Salts Are Mediated by TRPM5 and TRPV1 Channels

Sensory Attributes of Complex Tasting Divalent Salts Are Mediated by TRPM5 and TRPV1 Channels

Nasal chemosensory cells use bitter taste signaling to detect irritants and bacterial signals

Bitter triggers acetylcholine release from polymodal urethral chemosensory cells and bladder reflexes

Contact Info

Product

Resources

About