TRPM3 expression and control of glutamate release from primary vagal afferent neurons

Ragozzino, Forrest J.; Arnold, Rachel A.; Fenwick, Axel J.; Riley, Timothy P.; Lindberg, Jonathan E M; Peterson, BreeAnne; Peters, James H.

doi:10.1152/jn.00229.2020

Cited by 17 publications

(11 citation statements)

References 49 publications

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“…TRPM3 has been detected within the rat nodose ganglion and other regions of the nervous system including the nucleus of the solitary tract in the brainstem (Ragozzino et al, 2021). Their immunohistology images captured using a standard immunofluorescence microscope exhibit a diffuse expression of TRPM3 in neuron cell bodies and in neuronal fibers throughout the nodose ganglion.…”

Section: Discussionmentioning

confidence: 99%

Differential immunostaining patterns of transient receptor potential (TRP) ion channels in the rat nodose ganglion

et al. 2022

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Vagal afferents regulate numerous physiological functions including arterial blood pressure, heart rate, breathing, and nociception. Cell bodies of vagal afferents reside in the inferior vagal (nodose) ganglia and their stimulation by various means is being considered as a way to regulate cardiorespiratory responses and control pain sensations. Stimulation of the nodose by exposure to infrared light is recently being

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Section: Discussionmentioning

confidence: 99%

Differential immunostaining patterns of transient receptor potential (TRP) ion channels in the rat nodose ganglion

et al. 2022

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“…TRPM3 ion channel dysfunction in the pathology of both ME/CFS and long COVID suggests further research is required to determine whether the illnesses are separate. TRPM3 channels are widely expressed through multiple cell and tissue types and are highly expressed in the brainstem, thus may account for a common pathology in ME/CFS and long COVID (Held and Tóth, 2021;Ragozzino et al, 2021).…”

Section: Me/cfs Vs Long Covid Group Comparisonmentioning

confidence: 99%

Brainstem volume changes in myalgic encephalomyelitis/chronic fatigue syndrome and long COVID patients

et al. 2023

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Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) and long COVID patients have overlapping neurological, autonomic, pain, and post-exertional symptoms. We compared volumes of brainstem regions for 10 ME/CFS (CCC or ICC criteria), 8 long COVID (WHO Delphi consensus), and 10 healthy control (HC) subjects on 3D, T1-weighted MRI images acquired using sub-millimeter isotropic resolution using an ultra-high field strength of 7 Tesla. Group comparisons with HC detected significantly larger volumes in ME/CFS for pons (p = 0.004) and whole brainstem (p = 0.01), and in long COVID for pons (p = 0.003), superior cerebellar peduncle (p = 0.009), and whole brainstem (p = 0.005). No significant differences were found between ME/CFS and long COVID volumes. In ME/CFS, we detected positive correlations between the pons and whole brainstem volumes with “pain” and negative correlations between the midbrain and whole brainstem volumes with “breathing difficulty.” In long COVID patients a strong negative relationship was detected between midbrain volume and “breathing difficulty.” Our study demonstrated an abnormal brainstem volume in both ME/CFS and long COVID consistent with the overlapping symptoms.

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“…We currently do not know where the temperature sensing cells might be located in the organism. Support for TRPM3's role in driving peptide or small molecule release has previously been shown [68,69]. Several neuropeptides that modulate T. adhaerens have already been demonstrated [70], and the recent work of Nikitin et al presents a large amount of data on normal T. adhaerens locomotion and modulatory effects of amino acids transmitters and ATP [71].…”

Section: Discussionmentioning

confidence: 97%

Thermotaxis in an apolar, non-neuronal animal

Zhong,

Kroo,

Prakash

2023

J. R. Soc. Interface.

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Neuronal circuits are hallmarks of complex decision-making processes in the animal world. How animals without neurons process information and respond to environmental cues promises a new window into studying precursors of neuronal control and origin of the nervous system as we know it today. Robust decision making in animals, such as in chemotaxis or thermotaxis, often requires internal symmetry breaking (such as anterior–posterior (AP) axis) provided naturally by a given body plan of an animal. Here we report the discovery of robust thermotaxis behaviour in Trichoplax adhaerens , an early-divergent, enigmatic animal with no anterior–posterior symmetry breaking (apolar) and no known neurons or muscles. We present a quantitative and robust behavioural response assay in Placozoa , which presents an apolar flat geometry. By exposing T. adhaerens to a thermal gradient under a long-term imaging set-up, we observe robust thermotaxis that occurs over timescale of hours, independent of any circadian rhythms. We quantify that T. adhaerens can detect thermal gradients of at least 0.1°C cm −1 . Positive thermotaxis is observed for a range of baseline temperatures from 17°C to 22.5°C, and distributions of momentary speeds for both thermotaxis and control conditions are well described by single exponential fits. Interestingly, the organism does not maintain a fixed orientation while performing thermotaxis. Using natural diversity in size of adult organisms (100 µm to a few millimetres), we find no apparent size-dependence in thermotaxis behaviour across an order of magnitude of organism size. Several transient receptor potential (TRP) family homologues have been previously reported to be conserved in metazoans, including in T. adhaerens . We discover naringenin, a known TRPM3 antagonist, inhibits thermotaxis in T. adhaerens . The discovery of robust thermotaxis in T. adhaerens provides a tractable handle to interrogate information processing in a brainless animal. Understanding how divergent marine animals process thermal cues is also critical due to rapid temperature rise in our oceans.

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TRPM3 expression and control of glutamate release from primary vagal afferent neurons

Cited by 17 publications

References 49 publications

Differential immunostaining patterns of transient receptor potential (TRP) ion channels in the rat nodose ganglion

Differential immunostaining patterns of transient receptor potential (TRP) ion channels in the rat nodose ganglion

Brainstem volume changes in myalgic encephalomyelitis/chronic fatigue syndrome and long COVID patients

Thermotaxis in an apolar, non-neuronal animal

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