TRPC channels as signal transducers

Glazebrook, Patricia A.; Schilling, William P.; Kunze, Diana L.

doi:10.1007/s00424-005-1468-5

Cited by 45 publications

(39 citation statements)

References 27 publications

(30 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the NG, TRPC1-C7 expression has been reported in the soma of neurons and only TRPC1 and TRPC3-C5 expression has been observed in fibers in this ganglion (Glazebrook et al, 2005). In the same study, TrpC1 and C3, similar to our observation, exhibited strong staining in neuronal subpopulations with C1, C3, C4, and C5 present in myelinated A-type fibers and C1, C4, and C5 present in nonmyelinated C-type endings.…”

Section: Discussionsupporting

confidence: 88%

“…We were interested to examine if expressions of TRPC1-C7 were similar in thoracic DRG as in lumbar ganglia and to see if TRPC channels developmental expression was comparable in another sensory ganglia previously reported to express members of the TRPC family (Glazebrook et al, 2005). Real-time PCR was conducted on the thoracic DRG and NG collected from embryonic, postnatal, and adult stages.…”

Section: Expression Of Trpc Channels In Thoracic Drg and Ngmentioning

confidence: 99%

See 1 more Smart Citation

Cellular subtype distribution and developmental regulation of TRPC channel members in the mouse dorsal root ganglion

Elg

Marmigère

Mattsson

et al. 2007

J of Comparative Neurology

View full text Add to dashboard Cite

Transient receptor potential (TRP) channels play essential roles in sensory physiology and their expression in different classes of sensory neurons reflect distinct receptive properties of these neurons. While expression of the TRPV, TRPA, and to a certain degree TRPM classes of channels has been studied in sensory neurons, little is known about the expression and regulation of TRPC channels. In this study we examined the regulation of all TRPC members (TRPC1-C7) throughout embryonic and postnatal development of the dorsal root ganglion (DRG) and nodose ganglion (NG). In adult mice, mRNAs for all channels were present in the DRG, with TRPC1, 3, and 6 being the most abundant, TRPC2, C4, and C5 at lower levels, and TRPC7 at very low levels. While TRPC2 mRNAs were downregulated from high levels at embryonic (E) day 12 and E14 until adult, TRPC4, C5, and C7 expressions increased from E12 to peak levels at E18. TRPC1, C3, and C6, the most abundant TRPC channel mRNAs, increased progressively from E12 to adult. Expression and regulation of TRPC channels mRNAs in the NG were unexpectedly similar to the DRG. TRPC1 and C2 was expressed in the neurofilament-200 (NF-200)-positive large size subclass of neurons, while TRPC3 mRNAs expression, which stained up to 35% of DRG neurons, was almost exclusively present in nonpeptidergic isolectin B4 (IB4)-positive small size neurons that were largely TRPV1-negative. Our results suggest important roles of the TRPC family of channels in sensory physiology of both nociceptive as well as nonnociceptive classes of neurons.

show abstract

Section: Discussionsupporting

confidence: 88%

Section: Expression Of Trpc Channels In Thoracic Drg and Ngmentioning

confidence: 99%

Cellular subtype distribution and developmental regulation of TRPC channel members in the mouse dorsal root ganglion

Elg

Marmigère

Mattsson

et al. 2007

J of Comparative Neurology

View full text Add to dashboard Cite

show abstract

“…These data indicate that the functions of TRPC channels depend on the specific assembly of TRPC subunits, the tissue type, and the age of animals. TRPC expression in various cell types typically is not limited to a single member but a combination of various TRPC subunits (18,(42)(43)(44). In addition, the TRPC expression profile changes during development.…”

Section: Discussionmentioning

confidence: 99%

TRPC4 in Rat Dorsal Root Ganglion Neurons Is Increased after Nerve Injury and Is Necessary for Neurite Outgrowth

Huang

Richardson

et al. 2008

Journal of Biological Chemistry

View full text Add to dashboard Cite

Canonical transient receptor potential (TRPC) receptors are Ca2؉ -permeable cation channels that have a variety of physiological functions and may be involved in neuronal development and plasticity. We investigated the expression profile of TRPC channels in adult rat dorsal root ganglia (DRG) after nerve injury and examined the role of TRPC4 in neurite outgrowth in cultured DRG neurons. Sciatic nerve transection and microinjection of dibutyryl cAMP were employed to induce axonal regeneration in vivo. TRPC4 mRNA was significantly increased whereas TRPC1, TRPC3, TRPC6, and TRPC7 remained unaltered after nerve injury or dibutyryl-cAMP microinjection. The increases in TRPC4 transcript and protein were transient with maximal levels reached at 2 or 7 days, respectively. In addition, TRPC4 transcript in ND7/23 and NDC cells, hybrid cell lines derived from neonatal DRG and neuroblastoma, was substantially increased on differentiation, characterized by neurite outgrowth. In adult DRG, TRPC4 immunoreactivity was found in small and large neurons, and nerve injury increased the number of TRPC4-immunoreactive cells, particularly in large neurons. TRPC4 immunoreactivity was present in growth cones at various stages of DRG neurite outgrowth in vitro. Suppression of TRPC4 by a specific small interfering RNA or antisense significantly reduced the length of neurites in cultured DRG neurons. Expression of short hairpin RNA significantly down-regulated TRPC4 protein level and shortened neurite lengths in differentiated ND7/23 cells. The reduction in neurite lengths in ND7/23 cells was rescued by overexpression of human TRPC4. Our results suggest that TRPC4 contributes to axonal regeneration after nerve injury.

show abstract

“…The results of several studies, chiefly employing immunofluorescence, have identified those cell types in a particular organ in which TRPC1 is expressed. These include neurons (Goel et al 2002;Glazebrook et al 2005;von Bohlen-Und-Halbach et al 2005) the retina (Crousillac et al 2003) and kidney (Goel et al 2006). …”

Section: Expression Of Trpc1 In Tissues and Cellsmentioning

confidence: 98%

TRPC1 Ca2+-Permeable Channels in Animal Cells

Rychkov

Barritt

2007

Handbook of Experimental Pharmacology

View full text Add to dashboard Cite

The full-length transient receptor (TRPC)1 polypeptide is composed of about 790 amino acids, and several splice variants are known. The predicted structure and topology is of an integral membrane protein composed of six transmembrane domains, and a cytoplasmic C- and N-terminal domain. The N-terminal domain includes three ankyrin repeat motifs. Antibodies which recognise TRPC1 have been developed, but it has been difficult to obtain antibodies which have high affinity and specificity for TRPC1. This has made studies of the cellular functions of TRPC1 somewhat difficult. The TRPC1 protein is widely expressed in different types of animal cells, and within a given cell is found at the plasma membrane and at intracellular sites. TRPC1 interacts with calmodulin, caveolin-1, the InsP3 receptor, Homer, phospholipase C and several other proteins. Investigations of the biological roles and mechanisms of action of TRPC1 have employed ectopic (over-expression or heterologous expression) of the polypeptide in addition to studies of endogenous TRPC1. Both approaches have encountered difficulties. TRPC1 forms heterotetramers with other TRPC polypeptides resulting in cation channels which are non-selective. TRPC1 may be: a component of the pore of store-operated Ca2+ channels (SOCs); a subsidiary protein in the pathway of activation of SOCs; activated by interaction with InsP3R; and/or activated by stretch. Further experiments are required to resolve the exact roles and mechanisms of activation of TRPC1. Cation entry through the TRPC1 channel is feed-back inhibited by Ca2+ through interaction with calmodulin, and is inhibited by Gd3+, La3+, SKF96365 and 2-APB, and by antibodies targeted to the external mouth of the TRPC1 pore. Activation of TRPC1 leads to the entry to the cytoplasmic space of substantial amounts of Na+ as well as Ca2+. A requirement for TRPC1 is implicated in numerous downstream cellular pathways. The most clearly described roles are in the regulation of growth cone turning in neurons. It is concluded that TRPC1 is a most interesting protein because of the apparent wide variety of its roles and functions and the challenges posed to those attempting to elucidate its primary intracellular functions and mechanisms of action.

show abstract

TRPC channels as signal transducers

Cited by 45 publications

References 27 publications

Cellular subtype distribution and developmental regulation of TRPC channel members in the mouse dorsal root ganglion

Cellular subtype distribution and developmental regulation of TRPC channel members in the mouse dorsal root ganglion

TRPC4 in Rat Dorsal Root Ganglion Neurons Is Increased after Nerve Injury and Is Necessary for Neurite Outgrowth

TRPC1 Ca2+-Permeable Channels in Animal Cells

Contact Info

Product

Resources

About