TRP channels in mechanosensation

Lin, Sunny Jui-Shan; Corey, David P.

doi:10.1016/j.conb.2005.05.012

Cited by 126 publications

(72 citation statements)

References 41 publications

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“…Unfortunately there is little biophysical data on these channels to compare to the MET channel. Recently the TRPA1 channel has received a lot of attention as the potential MET channel (Corey et al, 2004;Gillespie, Dumont & Kachar, 2005;Lin & Corey, 2005). A variety of technically diverse data support this contention including in situ hybridization, immunocytochemistry, genetic strategies and RNAi to antagonize expression of the channel (Corey et al, 2004).…”

Section: Candidate Channelsmentioning

confidence: 99%

“…Whatever the identity of the gating spring, or its precise mode of engagement of the transducer channel (Kung, 2005;Lin & Corey, 2005), its biophysical consequences are well understood and may explain many observed properties of the transduction process in hair cells. Tension in the gating spring, resulting from the deflection of a hair bundle, is then transferred onto the transducer channels.…”

Section: Gating-spring Modelmentioning

confidence: 99%

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Mechano-electrical Transduction: New Insights into Old Ideas

et al. 2006

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The gating-spring theory of hair cell mechanotransduction channel activation was first postulated over twenty years ago. The basic tenets of this hypothesis have been reaffirmed in hair cells from both auditory and vestibular systems and across species. In fact, the basic findings have been reproduced in every hair cell type tested. A great deal of information regarding the structural, mechanical, molecular and biophysical properties of the sensory hair bundle and the mechanotransducer channel has accumulated over the past twenty years. The goal of this review is to investigate new data, using the gating spring hypothesis as the framework for discussion. Mechanisms of channel gating are presented in reference to the need for a molecular gating spring or for tethering to the intra-or extracellular compartments. Dynamics of the sensory hair bundle and the presence of motor proteins are discussed in reference to passive contributions of the hair bundle to gating compliance. And finally, the molecular identity of the channel is discussed in reference to known intrinsic properties of the native transducer channel.

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Section: Candidate Channelsmentioning

confidence: 99%

Section: Gating-spring Modelmentioning

confidence: 99%

Mechano-electrical Transduction: New Insights into Old Ideas

et al. 2006

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“…Characterization of ionic currents in MSCs shows that Ca 21 entry through the plasma membrane is mainly mediated by store operated Ca 21 channels, with a little contribution from voltage-gated Ca 21 channels [9][10][11]. The transient receptor potential channels (TRP channels) that reside in the plasma membrane are nonselective cation channels and thought to be candidates for mechanosensation [12,13]. We tested the hypothesis that TRP channels sensed mechanical stimulation and mediated the Ca 21 influx, resulting in osteogenesis in human bone marrow MSCs.…”

Section: Introductionmentioning

confidence: 99%

Brief Reports: TRPM7 Senses Mechanical Stimulation Inducing Osteogenesis in Human Bone Marrow Mesenchymal Stem Cells

et al. 2015

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Mesenchymal stem cells (MSCs) are multipotential stem cells residing in the bone marrow. Several studies have shown that mechanical stimulation modulates MSC differentiation through mobilization of second messengers, but the mechanism of mechanotransduction remains poorly understood. In this study, using fluorescence and laser confocal microcopy as well as patchclamp techniques, we identified the transient receptor potential melastatin type 7 (TRPM7) channel as the key channel involved in mechanotransduction in bone marrow MSCs. TRPM7 knockdown completely abolished the pressure-induced cytosolic Ca 21 increase and pressureinduced osteogenesis. TRPM7 directly sensed membrane tension, independent of the cytoplasm and the integrity of cytoskeleton. Ca 21 influx through TRPM7 further triggered Ca 21 release from the inositol trisphosphate receptor type 2 on the endoplasmic reticulum and promoted NFATc1 nuclear localization and osteogenesis. These results identified a central role of TRPM7 in MSC mechanical stimulation-induced osteogenesis. STEM CELLS 2015;33:615-621

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“…TRP channels gate in response to a variety of chemical and physical stimuli including pungent agents, temperature, pain, lipids, acid, and shear stress (Tominaga and Caterina, 2004;Lin and Corey, 2005;Pedersen et al, 2005;. Thus far, the TRP superfamily can be subdivided into seven families: TRPC, TRPV, TRPM, TRPP, TRPML, TRPA, and TRPN family (Clapham, 2003;Nilius and Voets, 2005;Minke, 2006).…”

Section: Introductionmentioning

confidence: 99%

Activation of TRPA1 Channel Facilitates Excitatory Synaptic Transmission in Substantia Gelatinosa Neurons of the Adult Rat Spinal Cord

Kosugi

Nakatsuka²,

Fujita³

et al. 2007

J. Neurosci.

134

109

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TRPA1 is expressed in primary sensory neurons and hair cells, and it is proposed to be activated by cold stimuli, mechanical stimuli, or pungent ingredients. However, its role in regulating synaptic transmission has never been documented yet. In the present study, we examined whether activation of the TRPA1 channels affects synaptic transmission in substantia gelatinosa (SG) neurons of adult rat spinal cord slices by using the whole-cell patch-clamp technique. A chief ingredient of mustard oil, allyl isothiocyanate (AITC), superfused for 2 min markedly increased the frequency and amplitude of spontaneous EPSCs (sEPSCs), which was accompanied by an inward current. Similar actions were produced by cinnamaldehyde and allicin. The AITC-induced increases in sEPSC frequency and amplitude were resistant to tetrodotoxin (TTX) and La 3ϩ , whereas being significantly reduced in extent in a Ca 2ϩ -free bath solution. In the presence of glutamate receptor antagonists CNQX and AP5, AITC did not generate any synaptic activities. The AITC-induced increases in sEPSC frequency and amplitude were reduced by ruthenium red, whereas being unaffected by capsazepine. AITC also increased the frequency and amplitude of spontaneous inhibitory postsynaptic currents; this AITC action was abolished in the presence of TTX or glutamate receptor antagonists. These results indicate that TRPA1 appears to be localized not only at presynaptic terminals on SG neurons to enhance glutamate release, but also in terminals of primary afferents innervating onto spinal inhibitory interneurons, which make synapses with SG neurons. This central modulation of sensory signals may be associated with physiological and pathological pain sensations.

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TRP channels in mechanosensation

Cited by 126 publications

References 41 publications

Mechano-electrical Transduction: New Insights into Old Ideas

Mechano-electrical Transduction: New Insights into Old Ideas

Brief Reports: TRPM7 Senses Mechanical Stimulation Inducing Osteogenesis in Human Bone Marrow Mesenchymal Stem Cells

Activation of TRPA1 Channel Facilitates Excitatory Synaptic Transmission in Substantia Gelatinosa Neurons of the Adult Rat Spinal Cord

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