TRP channel mediated neuronal activation and ablation in freely behaving zebrafish

Chen, Shijia; Chiu, Cindy N.; McArthur, Kimberly L.; Fetcho, Joseph R.; Prober, David A.

doi:10.1038/nmeth.3691

Cited by 60 publications

(77 citation statements)

References 31 publications

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“…Fourth, real-time monitoring of the activity levels of Hcrt neurons in freely behaving zebrafish larvae revealed that Hcrt neurons are wake active, similar to previous findings in rats and mice [25–27]. Finally, ablation of Hcrt neurons in zebrafish larvae results in increased and fragmented sleep [24•,28], reminiscent of narcolepsy endophenotypes.…”

Section: Hypocretinsupporting

confidence: 76%

“…Second, a recently developed high-throughput and non-invasive optogenetic assay demonstrated that stimulation of Hcrt neurons increases locomotor activity (Figure 1C) in a hcrtr dependent manner [23••], consistent with the arousing effect of optogenetic stimulation of Hcrt neurons in mice [18]. Third, chemogenetic stimulation of Hcrt neurons, again in freely-behaving zebrafish larvae, using the capsaicin-activated TRPV1 channel increases activity and reduces sleep [24•]. Fourth, real-time monitoring of the activity levels of Hcrt neurons in freely behaving zebrafish larvae revealed that Hcrt neurons are wake active, similar to previous findings in rats and mice [25–27].…”

Section: Hypocretinmentioning

confidence: 65%

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Attacking sleep from a new angle: contributions from zebrafish

Oikonomou

Prober

2017

Current Opinion in Neurobiology

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Sleep consumes a third of our lifespan, but we are far from understanding how it is initiated, maintained and terminated, or what purposes it serves. To address these questions, alternative model systems have recently been recruited. The diurnal zebrafish holds the promise of bridging the gap between simple invertebrate systems, which show little neuroanatomical conservation with mammals, and well-established, but complex and nocturnal, murine systems. Zebrafish larvae can be monitored in a high-throughput fashion, pharmacologically tested by adding compounds into the water, genetically screened using transient transgenesis, and optogenetically manipulated in a non-invasive manner. Here we discuss work that has established the zebrafish as a powerful system for the study of sleep, as well as novel insights gained by exploiting its particular advantages.

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Section: Hypocretinsupporting

confidence: 76%

Section: Hypocretinmentioning

confidence: 65%

Attacking sleep from a new angle: contributions from zebrafish

Oikonomou

Prober

2017

Current Opinion in Neurobiology

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“…We therefore tested several possible targeting strategies for caTRPA1 and found that they all, in fact, disturb the channel in some ways. In our experiments, C-terminal fusion of caTRPA1 with FP, although reported earlier as a thermogenetic tool in zebrafish21, demonstrated very poor performance compared to the N-terminal fusion or wild-type protein. This result can be attributed to the position of C-terminus in the structure of caTRPA1 essential for homotetramer formation56.…”

Section: Discussioncontrasting

confidence: 55%

Thermogenetic neurostimulation with single-cell resolution

et al. 2017

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Thermogenetics is a promising innovative neurostimulation technique, which enables robust activation of neurons using thermosensitive transient receptor potential (TRP) cation channels. Broader application of this approach in neuroscience is, however, hindered by a limited variety of suitable ion channels, and by low spatial and temporal resolution of neuronal activation when TRP channels are activated by ambient temperature variations or chemical agonists. Here, we demonstrate rapid, robust and reproducible repeated activation of snake TRPA1 channels heterologously expressed in non-neuronal cells, mouse neurons and zebrafish neurons in vivo by infrared (IR) laser radiation. A fibre-optic probe that integrates a nitrogen−vacancy (NV) diamond quantum sensor with optical and microwave waveguide delivery enables thermometry with single-cell resolution, allowing neurons to be activated by exceptionally mild heating, thus preventing the damaging effects of excessive heat. The neuronal responses to the activation by IR laser radiation are fully characterized using Ca2+ imaging and electrophysiology, providing, for the first time, a complete framework for a thermogenetic manipulation of individual neurons using IR light.

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“…chemogenetics) or with light (i.e. optogenetics) (Chen et al, 2016;Portugues et al, 2013). "Snapshots" of whole brain activity can also be taken using in situ hybridization of the immediate early gene c-fos, a marker of neuronal activity (Ellis et al, 2012), or with antibody staining for another activity marker, phosphorylated ERK (Randlett et al, 2015).…”

Section: Unique Advantages Of Zebrafish In Asd Modelingmentioning

confidence: 99%

The zebrafish as a promising tool for modeling human brain disorders: A review based upon an IBNS Symposium

Shams

Rihel

Ortíz

et al. 2018

Neuroscience & Biobehavioral Reviews

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The zebrafish represents an excellent compromise between system complexity and practical simplicity, features that make it useful for modeling and mechanistic analysis of complex brain disorders. Also promising are screens for psychoactive drugs with effects on larval and adult zebrafish behavior. This review, based upon a recent symposium held at the 2016 IBNS Congress, provides different perspectives on how the zebrafish may be utilized to advance research into human central nervous system disorders. It starts with a discussion on an important bottleneck in zebrafish research, measuring the behavior of this species (specifically shoaling), and continues with examples on research on autism spectrum disorder in larval zebrafish, on screening natural products for compounds with psychoactive properties in adult zebrafish, and on the development of a zebrafish model of fetal alcohol spectrum disorders. By providing information on a broad spectrum of brain disorders, experimental methods, and scientific approaches using both larval and adult zebrafish, the review is intended to showcase this underutilized laboratory species for behavioral neuroscience and psychopharmacology research.

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TRP channel mediated neuronal activation and ablation in freely behaving zebrafish

Cited by 60 publications

References 31 publications

Attacking sleep from a new angle: contributions from zebrafish

Attacking sleep from a new angle: contributions from zebrafish

Thermogenetic neurostimulation with single-cell resolution

The zebrafish as a promising tool for modeling human brain disorders: A review based upon an IBNS Symposium

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