Troubleshooting: Quantification of mobilization of progenitor cell subsets from bone marrow in vivo

Pitchford, Simon; Hahnel, Mark; Jones, Carla P.; Rankin, Sara M.

doi:10.1016/j.vascn.2010.01.013

Cited by 19 publications

(8 citation statements)

References 68 publications

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“…More recent studies (Pitchford et al 2009) have compared the effects of VEGF, G-CSF and AMD3100 on the mobilization of cells in mice, in part, confirming Shepherd et al's findings in the human. They used a novel in situ hind limb perfusion assay to collect mobilized cells and analysed these for late outgrowth endothelial cells, MSCs or stromal progenitor cells and haemopoietic progenitor cells Pitchford et al 2009Pitchford et al , 2010. G-CSF mobilization of late outgrowth endothelial cells was modest, and its effect was mostly on the mobilization of HSC/HPC.…”

Section: Mobilizing Factors In Animal Modelsmentioning

confidence: 99%

Human endothelial stem/progenitor cells, angiogenic factors and vascular repair

Watt

Athanassopoulos

Harris

et al. 2010

J. R. Soc. Interface.

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Neovascularization or new blood vessel formation is of utmost importance not only for tissue and organ development and for tissue repair and regeneration, but also for pathological processes, such as tumour development. Despite this, the endothelial lineage, its origin, and the regulation of endothelial development and function either intrinsically from stem cells or extrinsically by proangiogenic supporting cells and other elements within local and specific microenvironmental niches are still not fully understood. There can be no doubt that for most tissues and organs, revascularization represents the holy grail for tissue repair, with autologous endothelial stem/progenitor cells, their proangiogenic counterparts and the products of these cells all being attractive targets for therapeutic intervention. Historically, a great deal of controversy has surrounded the identification and origin of cells and factors that contribute to revascularization, the use of such cells or their products as biomarkers to predict and monitor tissue damage and repair or tumour progression and therapeutic responses, and indeed their efficacy in revascularizing and repairing damaged tissues. Here, we will review the role of endothelial progenitor cells and of supporting proangiogenic cells and their products, principally in humans, as diagnostic and therapeutic agents for wound repair and tissue regeneration.

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Section: Mobilizing Factors In Animal Modelsmentioning

confidence: 99%

Human endothelial stem/progenitor cells, angiogenic factors and vascular repair

Watt

Athanassopoulos

Harris

et al. 2010

J. R. Soc. Interface.

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“…In addition, MSCs can be obtained from various connective tissue sources, including bone marrow ( 12 ), adipose ( 13 ), dermal tissue ( 14 ), synovial fluid ( 15 ), deciduous teeth ( 16 , 17 ) and umbilical cord blood ( 18 ). Following adequate stimulation, stem and progenitor cells may leave the cell niche and enter the peripheral blood, which is termed stem cell mobilization ( 19 ). Although it has been confirmed that allograft MSCs, following in vitro amplification, can repair numerous types of tissue damage, it has been suggested that in vitro -amplified MSCs may induce the development of tumors ( 20 ), thus arousing doubts regarding the safety of allograft MSCs.…”

Section: Introductionmentioning

confidence: 99%

Characterization of bone marrow-derived mesenchymal stem cells from dimethyloxallyl glycine-preconditioned mice: Evaluation of the feasibility of dimethyloxallyl glycine as a mobilization agent

Liu

et al. 2016

Molecular Medicine Reports

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The prolyl hydroxylase inhibitor dimethyloxallyl glycine (DMOG) has been increasingly studied with regards to stem cell therapy. Previous studies have demonstrated that endogenous mesenchymal stem cells (MSCs) may be mobilized into peripheral circulation by pharmaceutical preconditioning. In addition, our previous study confirmed that DMOG, as a novel mobilization agent, could induce mouse/rat MSC migration into peripheral blood circulation. Therefore, the present study conducted studies to characterize bone marrow-derived MSCs (BM-MSCs) collected from mice following DMOG intraperitoneal injection. The surface antigen immune phenotype, differentiation capability, proliferative ability, migratory capacity and paracrine capacity of the BM-MSCs collected from DMOG-preconditioned mice (DBM-MSCs) or normal saline-treated mice (NBM-MSCs) were evaluated by means of flow cytometry, differentiation induction, Cell Counting kit-8, Transwell assay and enzyme-linked immunosorbent assay, respectively. Compared with NBM-MSCs, DBM-MSCs displayed a similar immune phenotype and multilineage differentiation capability, reduced proliferative ability and migratory capacity, and similar transforming growth factor and platelet-derived growth factor secretion capacity. These results provide a novel insight into the biological properties of BM-MSCs from mice preconditioned with DMOG. DBM-MSCs exhibited slightly distinct characteristics to NBM-MSCs; however, they may have therapeutic potential for future stem cell therapy. In addition, the present study suggested that DMOG may be used as a novel mobilization agent in future clinical trials as no adverse effects were observed.

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“…Experimental and clinical studies have revealed that bone marrow-derived progenitor cells can migrate into the circulation, incorporate into tumor microenvironments and contribute to the growth of various tumors ( 33 , 39 ). Chemotactic cytokines released by the damaged tissues mobilize progenitor cells by establishing mechanisms from the bone marrow, which may also regulate the mobilization of tissue-specific stem cells via other signaling pathways ( 40 ). Undifferentiated tumor cells may enter the circulation and result in distant solid tumor formation when the balance of a stem cell niche in various tissues is disrupted.…”

Section: Discussionmentioning

confidence: 99%

Intramuscular injection of bone marrow mononuclear cells contributes to bone repair following midpalatal expansion in rats

Che

Guo

et al. 2015

Molecular Medicine Reports

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Healing from injury requires the activation and proliferation of stem cells for tissue repair. Previous studies have demonstrated that bone marrow is a central pool of stem cells. The present study aimed to investigate the route undertaken by bone marrow mononuclear cells (BMMCs) following BMMC transplantation by masseter injection in a rat model of midpalatal expansion. The rats were divided into five groups according to the types of midpalatal expansion, incision and BMMC transplantation. Samples of midpalatal bone from the rats in each group were used for histological and immunohistochemical assessments to track and evaluate the differential potentials of the transplanted BMMCs in the masseter muscle and midpalatal bone. Bromodeoxyuridine was used as a BMMC tracing label, and M-cadherin was used to detect muscle satellite cells. The BMMCs injected into the masseter were observed, not only in the masseter, but also in the blood vessels and oral mucosa, and enveloped the midpalatal bone. A number of the BMMCs transformed into osteoblasts at the boundary of the neuromuscular bundle, and were embedded in the newly formed bone during midpalatal bone regeneration. The results of the present study suggested that BMMCs entered the circulation and migrated from muscle to the bone tissue, where they were involved in bone repair. Therefore, BMMCs may prove useful in the treatment of various types of cancer.

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Troubleshooting: Quantification of mobilization of progenitor cell subsets from bone marrow in vivo

Cited by 19 publications

References 68 publications

Human endothelial stem/progenitor cells, angiogenic factors and vascular repair

Human endothelial stem/progenitor cells, angiogenic factors and vascular repair

Characterization of bone marrow-derived mesenchymal stem cells from dimethyloxallyl glycine-preconditioned mice: Evaluation of the feasibility of dimethyloxallyl glycine as a mobilization agent

Intramuscular injection of bone marrow mononuclear cells contributes to bone repair following midpalatal expansion in rats

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