Trophoblast deportation part I: Review of the evidence demonstrating trophoblast shedding and deportation during human pregnancy

Askelund, Kathryn; Chamley, Larry

doi:10.1016/j.placenta.2011.07.081

Cited by 65 publications

(40 citation statements)

References 33 publications

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“…1). The results are in good agreement with the known facts describing trophoblast deportation during pregnancy [4] and its function as the fetal structure directly facing maternal blood.…”

Section: Tablesupporting

confidence: 90%

How to Characterize Fetal Cells from the Maternal Circulation?

Korabečná

Jirkovská²

2015

Fetal Diagn Ther

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“…1). The results are in good agreement with the known facts describing trophoblast deportation during pregnancy [4] and its function as the fetal structure directly facing maternal blood.…”

Section: Tablesupporting

confidence: 90%

How to Characterize Fetal Cells from the Maternal Circulation?

Korabečná

Jirkovská²

2015

Fetal Diagn Ther

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“…Our investigation of cellular fetal microchimerism adds to a substantial literature on the transfer of fetal material and deportation of trophoblast to the maternal circulation in pregnancy (reviewed in 12 and 20 ). Schmorl first demonstrated transfer of fetoplacental material to the maternal lung in eclamptic pregnancies in 1893.…”

Section: Discussionmentioning

confidence: 97%

Cellular Fetal Microchimerism in Preeclampsia

et al. 2013

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Previous studies have shown elevated concentrations of free fetal deoxyribonucleic acid and erythroblasts in maternal circulation in preeclampsia compared with normal pregnancy. Pluripotent and immunocompetent fetal cells also transfer to the maternal circulation during pregnancy, but whether concentrations of fetal mononuclear cells also differed in preeclampsia was unknown. We sought to quantify cellular fetal microchimerism in maternal circulation in women with preeclampsia and healthy controls. We studied women with preeclampsia and compared them with women with healthy pregnancies at similar gestational age. To identify a targetable polymorphism unique to the fetus to quantify fetal microchimerism, participants and family members were genotyped for the Human Leukocyte Antigen loci DRB1, DQA1, and DQB1, as well as several other polymorphisms. A panel of polymorphism-specific quantitative polymerase chain reaction assays was employed to identify and quantify fetal microchimerism in maternal peripheral blood mononuclear cells. Of 53 preeclampsia samples tested for cellular fetal microchimerism, 17 (32%) were positive, compared with 6 of 57 (6%) control samples (unadjusted odds ratio for detection 4.0, 95% confidence interval 1.5 – 11.1, p=0.007). The concentration of cellular fetal microchimerism (expressed as genome equivalents of fetal microchimerism per 100,000 maternal genome equivalents) was also higher among women with preeclampsia: median 0.0, mean 5.7, range 0-153.7, compared with controls: median 0.0, mean 0.3, range 0-9.1, p=0.002. We conclude that women with preeclampsia harbor cellular fetal microchimerism more commonly and at higher concentrations compared with women with uncomplicated pregnancy. The functional capacity and phenotype of these fetal cells is not yet known.

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“…There is great diversity in the size and composition of the expelled placental material. The material can be syncytial nuclear aggregates or large multinucleate deported syncytiotrophoblasts (20-100 mm) that are predominately retained in the pulmonary circulation (Askelund and Chamley 2011), several forms of apoptotic bodies (300-3000 nm) produced by blebbing of the plasma membrane (Redman et al 2012), cell-free fetal DNA circulating in the serum that is becoming important for non-invasive prenatal testing (Taglauer et al 2014), as well as extracellular vesicles (30-1000 nm; Redman et al 2012). It has recently been shown that pulmonary diseases are predisposing for development of PE (Thomsen 2015) possibly due to altered scavenging of syncytiotrophoblast microvesicles by the lung.…”

Section: Extracellular Vesicles and Their Characteristicsmentioning

confidence: 99%

Placenta-derived extracellular vesicles: their cargo and possible functions

Familari

Cronqvist

Masoumi

et al. 2017

Reprod. Fertil. Dev.

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The literature on extracellular vesicles consists of rapidly expanding and often contradictory information. In this paper we attempt to review what is currently known regarding extracellular vesicles released specifically from human placental syncytiotrophoblast cells with a focus on the common but complex pregnancy-associated syndrome pre-eclampsia, where the level of syncytiotrophoblast extracellular vesicle release is significantly increased. We review common methods for syncytiotrophoblast extracellular vesicle derivation and isolation and we discuss the cargo of syncytiotrophoblast extracellular vesicles including proteins, RNA and lipids and their possible functions. A meta-analysis of available trophoblast-derived extracellular vesicle proteomic datasets revealed only three proteins in common: albumin, fibronectin-1 and plasminogen activator inhibitor-1, suggesting some variability in vesicle cargo, most likely reflecting stage and cell type of origin. We discuss the possible sources of variability that may have led to the low number of common markers, which has led us to speculate that markers and density in common use may not be strict criteria for identifying and isolating placenta-derived exosomes.

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Trophoblast deportation part I: Review of the evidence demonstrating trophoblast shedding and deportation during human pregnancy

Cited by 65 publications

References 33 publications

How to Characterize Fetal Cells from the Maternal Circulation?

How to Characterize Fetal Cells from the Maternal Circulation?

Cellular Fetal Microchimerism in Preeclampsia

Placenta-derived extracellular vesicles: their cargo and possible functions

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