Trophic Effect of Multiple Growth Factors in Amniotic Fluid or Human Milk on Cultured Human Fetal Small Intestinal Cells

Hirai, Chie; Ichiba, Hiroyuki; Sillanpää, Mika; Shintaku, Haruo; Yamano, Tsunekazu; Kusuda, Satoshi

doi:10.1097/00005176-200205000-00010

Cited by 142 publications

(101 citation statements)

References 19 publications

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“…17 Trophic effects of AF have further been demonstrated on cultured human fetal small intestinal cells. 18 These studies suggest that growth factors found in AF, comparable to those in human milk, play a role in fetal growth and development. These trophic mediators are discussed below.…”

Section: Nutritive Functions Of Afmentioning

confidence: 96%

“…Recombinant TGF-a elicits a synergistic trophic response on cultured intestinal cells when combined with recombinant EGF, insulin-like growth factor-1, fibroblastic growth factor, and hepatocyte growth factor, but the trophic response is not as strong as either AF or breast milk. 18 The amnion cells of the umbilical cord express EGF, TGF-a, and the functional EGF/TGF-a receptor suggesting the possibility of a regulating role of the amnion in fetal growth and development. EGF and TGF-a have also been shown to stimulate the production of surfactant components.…”

Section: Nutritive Functions Of Afmentioning

confidence: 99%

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Amniotic Fluid: Not Just Fetal Urine Anymore

2005

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Section: Nutritive Functions Of Afmentioning

confidence: 96%

Section: Nutritive Functions Of Afmentioning

confidence: 99%

Amniotic Fluid: Not Just Fetal Urine Anymore

2005

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“…Exogenous infusion of EGF in utero has been shown to accelerate the maturation of intestine enzyme activity as well as stimulate intestinal growth [22]. Interestingly, the salivary level of EGF is directly proportional to the gestational age of the infant [23]. Hepatocyte Growth Factor (HGF) and Vascular Endothelial Growth Factor (VEGF) are expressed in gut tissues and play a major role in the mucosal growth and repair [24].…”

Section: Physiology Of Gut Maturationmentioning

confidence: 99%

“Human Milk Component (HMC) Therapy”: A Novel Treatment for Gut Maturation of Preterm Infants

Manohar¹,

Tukaram²,

Satish³

et al. 2014

J Neonatal Biol

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Even though the process of gut maturation starts right from intrauterine life, it really gets accelerated only after birth through enteral route. The preterm infants as well as infants with intrauterine growth restriction and those who had absent or reversed umbilical cord flow during their intrauterine stay are the candidates to have immature gut. They are also prone to the complications like feeding intolerance and necrotizing enterocolities.It is well established that the human milk due to the presence of growth factors and cellular components, play important role in the process of gut maturation. With this background, we have hypothesised and proposed the concept of Human Milk Component (HMC) isolation and therapy for gut maturation in preterm infants. The aqueous fraction of human milk rich in growth factors and bioactive substances and the cellular fraction rich in different kinds of cells including mesenchymal stem cells are isolated. They are given through the feeding tube to the infants with immature gut.The aqueous component of human milk acts by a passive mechanism while the cellular component acts by an active mechanism. This concept further strengthens the importance of human milk.

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“…For studies of human milk-derived bioactives, the small intestinal FHs 74 Int. cell line has been widely used as a model (Ichiba et al, 1992;Kawamura et al, 1994;Wagner et al, 1998;Hirai et al, 2002;Takeda et al, 2004). We have shown that these small intestinal cells represent a very sensitive model for milk-derived bioactives (Purup et al, 2007).…”

Section: Cell-based Models Of the Gastrointestinal Tractmentioning

confidence: 99%

Cell-based models to test the effects of milk-derived bioactives

Purup

Nielsen²

2012

Animal

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The life science industries have a strong interest in screening for novel bioactives in complex mixtures like milk and dairy products. Food bioactives are not only important for public health in general, but also have potential therapeutic applications for the treatment of a number of diseases. To identify these novel bioactives, establishment of robust screening assays is essential. The use of in vitro cell-based models for screening and testing have the advantage that several concentrations of mixtures or specific compounds can be assayed at the same time in cells from specific tissues. Primary cell cultures from target organs or established cell lines can be used to identify the most sensitive cells. In addition, a large number of transfected cell lines with very specific sensitivities have been developed. Different endpoints inherent to basal or more sophisticated cellular functions can be investigated, such as cell viability, apoptosis, migration, intracellular signalling, regulation of gene expression, morphology and metabolic alterations. The gastrointestinal tract is an obvious target for bioactive molecules delivered through milk and dairy products, because it lies at the interface between dietary components in the lumen and the internal processes of the host. Identification of bioactive factors that affects proliferation or migration of epithelial cells may have potential applications in promoting gastrointestinal health in both humans and animals. The mammary gland is another target organ of considerable interest since it has been estimated that up to 50% of all newly diagnosed breast cancers may be related to dietary factors. A large number of gastrointestinal and mammary epithelial cell lines are commercially available, but in order to study some cellular functions, primary cultures of freshly isolated cells are often preferred, as established cell lines do not always express specialised properties in culture.

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Trophic Effect of Multiple Growth Factors in Amniotic Fluid or Human Milk on Cultured Human Fetal Small Intestinal Cells

Abstract: In aminiotic fluid and in human milk, EGF, IGF-1, FGF, HGF, and TGF-alpha have a strong trophic effect on immature intestinal cells and may be involved in perinatal gastrointestinal adaptation.

Cited by 142 publications

References 19 publications

Amniotic Fluid: Not Just Fetal Urine Anymore

Amniotic Fluid: Not Just Fetal Urine Anymore

“Human Milk Component (HMC) Therapy”: A Novel Treatment for Gut Maturation of Preterm Infants

Cell-based models to test the effects of milk-derived bioactives

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