‘Trophic’ and ‘source’ amino acids in trophic estimation: a likely metabolic explanation

O’Connell, Tamsin C.

doi:10.1007/s00442-017-3881-9

Cited by 150 publications

(174 citation statements)

References 112 publications

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“…, the algal and vascular plant primary producers were near the line where the TP Glu‐Phe values are equal to 1, with the corresponding β values. The precision levels (SD and potential uncertainty) of the TP Glu‐Phe of primary producers in this study are almost identical to the values that have been reported previously (Chikaraishi et al ; O'Connell ). Carnivorous fishes always lie at a higher position irrespective of the chosen β values.…”

Section: Discussionsupporting

confidence: 90%

“…Compound‐specific isotope analysis of amino acids (CSIA‐AA) has been widely used to estimate the TP of organisms in the aquatic and terrestrial ecosystem because different amino acids record both the TP and nitrogen isotopic baseline information (Chikaraishi et al ; O'Connell ; Ohkouchi et al ). Differential 15 N enrichment levels between the two groups amino acids (i.e., the trophic and source groups) in single trophic stepwise represent the core of the CSIA‐AA approach and the study of trophic ecology (McClelland and Montoya ; Chikaraishi et al ).…”

Section: βCorr and The Contribution Of Vascular Plant As A Diet Resoumentioning

confidence: 99%

“…However, the commonly used TEF values proposed by Chikaraishi et al () have been challenged in recent studies (Nielsen et al ; McMahon and McCarthy ). These studies suggested that the TEF value is variable and usually lower than 7.6‰ and indicated that a single TEF value is unlikely to realize the full potential of the CSIA‐AA method applied to assess trophic ecology (Blanke et al ; O'Connell ). The large variations of TEF values are driven by two biochemical factors, i.e., the feeding ecology and nitrogen excretion mode, which makes it the most sensitive parameter for the estimation of the TP (Gustine et al ; Décima et al ; Ohkouchi et al ).…”

Section: βCorr and The Contribution Of Vascular Plant As A Diet Resoumentioning

confidence: 99%

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Elucidating food web structure of the Poyang Lake ecosystem using amino acid nitrogen isotopes and Bayesian mixing model

Zhang

Tian

Cao

et al. 2019

Limnology & Ocean Methods

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Compound‐specific isotope analysis of amino acids (CSIA‐AA) is regarded as a more advanced method of unraveling food web connections than the traditional bulk isotope approach. One of the most important assumptions of the CSIA‐AA approach is that the initial offset (β) between glutamate and phenylalanine in primary producers should remain unchanged. However, the considerable difference in β values between algae (+3.4‰) and vascular plants (−8.0‰) raises concern regarding the application of the CSIA‐AA approach for complex ecosystems that depend on both resources. In the present study, a Bayesian mixing model was used to estimate the individual contribution of basal dietary resources to each consumer of Poyang Lake, and then, the β was reevaluated to adapt to the assimilation of the individual primary producer for each consumer specimen. In general, the contributions of vascular plant‐derived resources to most consumers are consistent with their feeding behaviors. The smaller contribution of vascular plants to planktivorous fish (usually less than 20%) is biologically expected because of their feeding ecology. Using this strategy, we successfully obtained realistic trophic positions (TP) of consumers in Poyang Lake, which are significantly different from traditional TPalgae or TPvascular. For instance, the TP value of grass carp (2.04) was consistent with the feeding behavior of this fish, i.e., they primarily feed on vascular hydrophytes. Therefore, the combination of the CSIA‐AA and Bayesian mixing model provides a better understanding of food web structures, even in a complex freshwater ecosystem.

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Section: Discussionsupporting

confidence: 90%

Section: βCorr and The Contribution Of Vascular Plant As A Diet Resoumentioning

confidence: 99%

Section: βCorr and The Contribution Of Vascular Plant As A Diet Resoumentioning

confidence: 99%

See 1 more Smart Citation

Elucidating food web structure of the Poyang Lake ecosystem using amino acid nitrogen isotopes and Bayesian mixing model

Zhang

Tian

Cao

et al. 2019

Limnology & Ocean Methods

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“…Results show it was possible to infer a recent drop in trophic level (rather than a change in nitrogen fixation) that may be attributable to industrial fishing. Amino‐acid‐specific δ 15 N analysis is an emerging field with its own methodological challenges (O'Connell, ), but one with considerable potential.…”

Section: Long‐term Changes In the Aquatic Environmentmentioning

confidence: 99%

An environmental (pre)history of European fishing: past and future archaeological contributions to sustainable fisheries

Barrett

2019

Journal of Fish Biology

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This paper explores the past and potential contribution of archaeology to marine historical ecology. The primary focus is European fishing of marine and diadromous taxa, with global comparisons highlighting the wider applicability of archaeological approaches. The review illustrates how study of excavated fish bones, otoliths and shells can inform our understanding of: (a) changes in biogeography, including the previous distribution of lost species; (b) long‐term fluctuations in the aquatic environment, including climate change; (c) the intensity of exploitation and other anthropogenic effects; (d) trade, commodification and globalisation. These issues are also relevant to inform fisheries conservation and management targets. Equally important, the long (pre)history of European fishing raises awareness of our ecological heritage debt, owed for centuries of wealth, sustenance and well‐being, and for which we share collective responsibility. This debt represents both a loss and a reason for optimism, insofar as it is a reservoir of potential to be filled by careful stewardship of our rivers, lakes, seas and oceans.

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“…Thus, source amino acids can be used to measure the δ 15 N values of primary producers, which is governed by regional patterns in nitrogen cycling processes (e.g., nitrogen fixation, denitrification, nitrification, nitrate assimilation). We can interpret ecological relationships using these basic patterns in amino acids, and with some consideration of the physiological and biochemical reactions that also influence isotope ratios …”

Section: Introductionmentioning

confidence: 99%

Effects of chemical preservation on bulk and amino acid isotope ratios of zooplankton, fish, and squid tissues

Hetherington

Kurle

Ohman

et al. 2019

Rapid Comm Mass Spectrometry

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Rationale It is imperative to understand how chemical preservation alters tissue isotopic compositions before using historical samples in ecological studies. Specifically, although compound‐specific isotope analysis of amino acids (CSIA‐AA) is becoming a widely used tool, there is little information on how preservation techniques affect amino acid δ15N values. Methods We evaluated the effects of chemical preservatives on bulk tissue δ13C and δ15N and amino acid δ15N values, measured by gas chromatography/isotope ratio mass spectrometry (GC/IRMS), of (a) tuna (Thunnus albacares) and squid (Dosidicus gigas) muscle tissues that were fixed in formaldehyde and stored in ethanol for 2 years and (b) two copepod species, Calanus pacificus and Eucalanus californicus, which were preserved in formaldehyde for 24–25 years. Results Tissues in formaldehyde‐ethanol had higher bulk δ15N values (+1.4, D. gigas; +1.6‰, T. albacares), higher δ13C values for D. gigas (+0.5‰), and lower δ13C values for T. albacares (−0.8‰) than frozen samples. The bulk δ15N values from copepods were not different those from frozen samples, although the δ13C values from both species were lower (−1.0‰ for E. californicus and −2.2‰ for C. pacificus) than those from frozen samples. The mean amino acid δ15N values from chemically preserved tissues were largely within 1‰ of those of frozen tissues, but the phenylalanine δ15N values were altered to a larger extent (range: 0.5–4.5‰). Conclusions The effects of preservation on bulk δ13C values were variable, where the direction and magnitude of change varied among taxa. The changes in bulk δ15N values associated with chemical preservation were mostly minimal, suggesting that storage in formaldehyde or ethanol will not affect the interpretation of δ15N values used in ecological studies. The preservation effects on amino acid δ15N values were also mostly minimal, mirroring bulk δ15N trends, which is promising for future CSIA‐AA studies of archived specimens. However, there were substantial differences in phenylalanine and valine δ15N values, which we speculate resulted from interference in the chromatographic resolution of unknown compounds rather than alteration of tissue isotopic composition due to chemical preservation.

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‘Trophic’ and ‘source’ amino acids in trophic estimation: a likely metabolic explanation

Cited by 150 publications

References 112 publications

Elucidating food web structure of the Poyang Lake ecosystem using amino acid nitrogen isotopes and Bayesian mixing model

Elucidating food web structure of the Poyang Lake ecosystem using amino acid nitrogen isotopes and Bayesian mixing model

An environmental (pre)history of European fishing: past and future archaeological contributions to sustainable fisheries

Effects of chemical preservation on bulk and amino acid isotope ratios of zooplankton, fish, and squid tissues

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