tRNA's and priming of RNA-directed DNA synthesis in mouse mammary tumor virus

Abstract

Purified MuMTV spot 1 RNA was subjected to complete hydrolysis with either RNase T1 or pancreatic RNase, and the resulting oligonucleotides were separated by two-dimensional electrophoresis (Fig. 3). Each oligonucleotide was further characterized by redigestion with RNases of different specificity.

The HIV plus-strand transfer reaction: determination of replication-competent intermediates and identification of a novel lentiviral element, the primer over-extension sequence

“…This could indicate that, in EIAV, RT may rarely, but selectively misincorporate C opposite the non-informative m1A58 modi®cation, and that only the four nucleotide over-extended product observed in our in vitro system is produced in vivo. Mouse mammary tumor virus (MMTV), a mammalian type B retrovirus, also uses tRNA 3 lys as primer, 31 but clearly does not contain a POS. None of the MMTV sequences examined contained any nucleotides complementary to products that would result from reverse transcription beyond m1A58 in the ®ve nucleotide stretch corresponding to the lentiviral POS (Table 1).…”

Primer tRNAs for reverse transcription

“…, representing two tRNA Lys isoacceptors differing by 1 bp in the anticodon stem, is the primer tRNA for several retroviruses, including Mason-Pfizer monkey virus and human foamy virus (HFV) (41). tRNA 3 Lys serves as the primer for mouse mammary tumor virus (62,82) and the lentiviruses, such as equine infectious anemia virus, feline immunodeficiency virus, simian immunodeficiency virus, and human immunodeficiency virus types 1 and 2 (HIV-1 and HIV-2) (41). tRNA Pro is the common primer for Moloney murine leukemia virus (Mo-MuLV) (20,61,77), but murine retroviruses have also been reported to make use of several alternate tRNAs as primers.…”

Long terminal repeat sequences of intracisternal A particle genes in the Syrian hamster genome: identification of tRNAPheas a putative primer tRNA

“…Three kinds of tRNA, Trp(36), Pro (37) and Lys (38), have been identified as primer tRNAs for reverse transcription. The nucleotide sequences of the primer-binding site of the two hamster IAP genes did not coincide with those A. Asterisks indicate nucleotide identity with the H10 sequence.…”