tRNA-modifying MiaE protein from<i>Salmonella typhimurium</i>is a nonheme diiron monooxygenase

Mathevon, Carole; Pierrel, Fabien; Oddou, Jean-Louis; García-Serres, Ricardo; Blondin, Geneviève; Latour, Jean‐Marc; Ménage, Stéphane; Gambarelli, Serge; Fontecave, Marc; Atta, Mohamed

doi:10.1073/pnas.0704338104

Cited by 44 publications

(56 citation statements)

References 55 publications

(61 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, the methylthiol pathway has not been readily characterized and 2MeSCKs are not detected in U. maydis axenic culture. Among the 2MeSCK pathway enzymes (Esberg et al ., ; Mathevon et al ., ), only the MiaC‐like enzyme (UMAG_05632) was detected in U. maydis . As outlined in Persson & Bjork (), MiaB and MiaC work together in the methylthiolation of tRNA.…”

Section: Discussionmentioning

confidence: 93%

Fungal derived cytokinins are necessary for normal Ustilago maydis infection of maize

2016

View full text Add to dashboard Cite

Phytohormones derived from fungi play a key role in regulating plant-pathogen interactions; however, deciphering the separate contributions of pathogen and plant during infection has been difficult. Here, the Ustilago maydis-Zea mays pathosystem was used to investigate this chemical exchange. Ustilago maydis, the causative agent of maize smut, produces cytokinins (CK), which are a group of phytohormones responsible for directing plant development. The characteristic symptom of smut disease is the formation of tumours composed of plant and fungal tissue. Isopentenyltransferase (IPT) catalyses the rate-limiting step in CK biosynthesis, and U. maydis strains in which the sole tRNA-ipt gene was deleted no longer produced CKs. These deletion strains elicited fewer, smaller tumours than the pathogenic strain SG200. High performance liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI MS/MS) was used to detect and quantify phytohormone levels in infected tissue. This revealed that key hormone changes in SG200 infections were not present in infections by deletion strains, suggesting that CK production by U. maydis is required for the altered phytohormone profile in infected tissue relative to uninfected tissue. Separate analyses indicated that U. maydis tRNA-ipt mutants might be altered in their ability to metabolize CKs taken up from the environment. Mining the U. maydis genome identified genes encoding putative CK signalling and biosynthesis proteins.

show abstract

Section: Discussionmentioning

confidence: 93%

Fungal derived cytokinins are necessary for normal Ustilago maydis infection of maize

2016

View full text Add to dashboard Cite

show abstract

“…Among the recent discoveries, can be found: -The MiaE tRNA monooxygenase, isolated from Salmonella typhimurium, that is responsible for the post-translational tRNA modification by allylic hydroxylation of 2-methylthio-N-6-isopentyladenosine [126,127]. -The PhnZ HD phosphohydroxylase, that catalyzes the conversion of 2-aminoethylphosphonate to 2-amino-1-hydroxyethylphosphonate (a phosphate source for aquatic bacteria) [128,129].…”

Section: Other Enzymes Identified As Diiron Oxygenasesmentioning

confidence: 99%

A growing family of O2 activating dinuclear iron enzymes with key catalytic diiron(III)-peroxo intermediates: Biological systems and chemical models

Trehoux

Mahy

Avenier

2016

Coordination Chemistry Reviews

View full text Add to dashboard Cite

“…Another gene, miaE, encoding tRNA 2-methylthio-N 6 -isopentenyladenosine cis-hydroxylase, was found in Salmonella typhimurium (Persson and Björk, 1993), but it is a rare gene, present neither in E. coli (Persson and Bjö rk, 1993) nor in eukaryotes (our BLAST search). The gene product was later characterized as a non-haem di-iron monooxygenase (Mathevon et al, 2007).…”

Section: Plant Parasitic Bacteria-cytokinin As a Tool For Invasionmentioning

confidence: 99%

Evolution of cytokinin biosynthesis and degradation

Frébort

Kowalska

Hluska

et al. 2011

Journal of Experimental Botany

349

283

View full text Add to dashboard Cite

Cytokinin hormones are important regulators of development and environmental responses of plants that execute their action via the molecular machinery of signal perception and transduction. The limiting step of the whole process is the availability of the hormone in suitable concentrations in the right place and at the right time to interact with the specific receptor. Hence, the hormone concentrations in individual tissues, cells, and organelles must be properly maintained by biosynthetic and metabolic enzymes. Although there are merely two active cytokinins, isopentenyladenine and its hydroxylated derivative zeatin, a variety of conjugates they may form and the number of enzymes/isozymes with varying substrate specificity involved in their biosynthesis and conversion gives the plant a variety of tools for fine tuning of the hormone level. Recent genome-wide studies revealed the existence of the respective coding genes and gene families in plants and in some bacteria. This review summarizes present knowledge on the enzymes that synthesize cytokinins, form cytokinin conjugates, and carry out irreversible elimination of the hormones, including their phylogenetic analysis and possible variations in different organisms.

show abstract

tRNA-modifying MiaE protein fromSalmonella typhimuriumis a nonheme diiron monooxygenase

Cited by 44 publications

References 55 publications

Fungal derived cytokinins are necessary for normal Ustilago maydis infection of maize

Fungal derived cytokinins are necessary for normal Ustilago maydis infection of maize

A growing family of O2 activating dinuclear iron enzymes with key catalytic diiron(III)-peroxo intermediates: Biological systems and chemical models

Evolution of cytokinin biosynthesis and degradation

Contact Info

Product

Resources

About