tRNA genes of Streptomyces lividans: new sequences and comparison of structure and organization with those of other bacteria

Sedlmeier, Reinhard; Werner, Thomas; Kieser, Helen M.; Hopwood, David A.; Schmieger, Horst

doi:10.1128/jb.176.17.5550-5553.1994

Cited by 10 publications

(3 citation statements)

References 41 publications

(39 reference statements)

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“…We also found that for the majority of cloned 3′ halves, there was evidence supporting the existence of a terminal CCA sequence, with either the entire CCA sequence being present or some portion of it (Supplementary Table 1). The terminal CCA sequence is added post-transcriptionally to the majority of tRNAs in S. coelicolor ( 25 ), in contrast to the situation in E. coli , where the terminal CCA is encoded within all tRNA genes ( 26 ). This suggested that the tRNA cleavage products were derived from mature tRNAs that were capable of being aminoacylated, and not from immature/precursor tRNA molecules.…”

Section: Resultsmentioning

confidence: 99%

Developmentally regulated cleavage of tRNAs in the bacterium Streptomyces coelicolor

Haiser¹,

Karginov²,

Hannon³

et al. 2007

Nucleic Acids Research

117

116

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The ability to sense and respond to environmental and physiological signals is critical for the survival of the soil-dwelling Gram-positive bacterium Streptomyces coelicolor. Nutrient deprivation triggers the onset of a complex morphological differentiation process that involves the raising of aerial hyphae and formation of spore chains, and coincides with the production of a diverse array of clinically relevant antibiotics and other secondary metabolites. These processes are tightly regulated; however, the genes and signals involved have not been fully elucidated. Here, we report a novel tRNA cleavage event that follows the same temporal regulation as morphological and physiological differentiation, and is growth medium dependent. All tRNAs appear to be susceptible to cleavage; however, there appears to be a bias towards increased cleavage of those tRNAs that specify highly utilized codons. In contrast to what has been observed in eukaryotes, accumulation of tRNA halves in S. coelicolor is not significantly affected by amino acid starvation, and is also not affected by induction of the stringent response or inhibition of ribosome function. Mutants defective in aerial development and antibiotic production exhibit altered tRNA cleavage profiles relative to wild-type strains.

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Section: Resultsmentioning

confidence: 99%

Developmentally regulated cleavage of tRNAs in the bacterium Streptomyces coelicolor

Haiser¹,

Karginov²,

Hannon³

et al. 2007

Nucleic Acids Research

117

116

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“…Analysis of the association between tRNA genes and rRNA genes in Streptomyces revealed a striking paradigm, with no tRNAs so far found to be associated with any rRNA operons. Sedlmeier and collaborators (204) proposed that the lack of tRNA genes with rRNA genes might be characteristic for all actinomycetes. Based on the first reports that initiator tRNAs from Streptomyces griseus and S. rimosus lack CCA ends (74, 128) and on later publications indicating that potential tRNA Pro , tRNA Thr , and tRNA Tyr genes associated with site-specific recombination in Streptomyces also lack CCA termini (132,143,197), Plohl and Gamulin (185) predicted that most, if not all, tRNA genes in S. rimosus do not encode a CCA terminus.…”

Section: Trna Rrna and Gene Expression In S Rimosusmentioning

confidence: 99%

Genetics ofStreptomyces rimosus, the Oxytetracycline Producer

Petković

Cullum

Hranueli

et al. 2006

Microbiol Mol Biol Rev

101

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SUMMARY From a genetic standpoint, Streptomyces rimosus is arguably the best-characterized industrial streptomycete as the producer of oxytetracycline and other tetracycline antibiotics. Although resistance to these antibiotics has reduced their clinical use in recent years, tetracyclines have an increasing role in the treatment of emerging infections and noninfective diseases. Procedures for in vivo and in vitro genetic manipulations in S. rimosus have been developed since the 1950s and applied to study the genetic instability of S. rimosus strains and for the molecular cloning and characterization of genes involved in oxytetracycline biosynthesis. Recent advances in the methodology of genome sequencing bring the realistic prospect of obtaining the genome sequence of S. rimosus in the near term.

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“…It is common for bacterial tRNAs to be encoded by multicistronic operons. A multicistronic tRNA transcript may contain more than one tRNA, or rRNA and mRNA in addition to tRNA (Wawrousek and Hansen 1983;Komine et al 1990;Sedlmeier et al 1994). Some bacteria have genetically encoded 3Ј terminal CCA for all tRNAs, whereas others only encode CCA for fractions of their total tRNAs (Deutscher 1984;Minagawa et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Co-evolution of tRNA 3′ trailer sequences with 3′ processing enzymes in bacteria

Gong²,

Joshi³

et al. 2005

RNA

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Maturation of the tRNA 3 terminus is a complicated process in bacteria. Usually, it is initiated by an endonucleolytic cleavage carried out by RNase E and Z in different bacteria. In Escherichia coli, RNase E cleaves AU-rich sequences downstream of tRNA, producing processing intermediates with a few extra residues at the 3 end; these are then removed by exoribonuclease trimming to generate the mature 3 end. Here we show that essentially all E. coli tRNA precursors contain a potential RNase E cleavage site, the AU-rich sequence element (AUE), in the 3 trailer. This suggests that RNase E cleavage and exonucleolytic trimming is a general pathway for tRNA maturation in this organism. Remarkably, the AUE immediately downstream of each tRNA is selectively conserved in bacteria having RNase E and tRNA-specific exoribonucleases, suggesting that this pathway for tRNA processing is also commonly used in these bacteria. Two types of RNase E-like proteins are identified in actinobacteria and the ␣-subdivision of proteobacteria. The tRNA 3 proximal AUE is conserved in bacteria with only one type of E-like protein.Selective conservation of the AUE is usually not observed in bacteria without RNase E. These results demonstrate a novel example of co-evolution of RNA sequences with processing activities.

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tRNA genes of Streptomyces lividans: new sequences and comparison of structure and organization with those of other bacteria

Cited by 10 publications

References 41 publications

Developmentally regulated cleavage of tRNAs in the bacterium Streptomyces coelicolor

Developmentally regulated cleavage of tRNAs in the bacterium Streptomyces coelicolor

Genetics ofStreptomyces rimosus, the Oxytetracycline Producer

Co-evolution of tRNA 3′ trailer sequences with 3′ processing enzymes in bacteria

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