TrkA‐immunoreactive profiles in the central nervous system: Colocalization with neurons containing p75 nerve growth factor receptor, choline acetyltransferase, and serotonin

Sobreviela, T.; Clary, Douglas O.; Reichardt, Louis F.; Brandabur, Melanie; Kordower, Jeffrey H.; Mufson, Elliott J.

doi:10.1002/cne.903500407

Cited by 304 publications

(219 citation statements)

References 96 publications

(161 reference statements)

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“…Immunostaining for NGF within the infusion site showed that exogenous NGF was con¢ned mainly to the cortical neuropil and seemed to avoid cortical neuronal cell bodies. We did not observe any TrkA immunoreactivity in cortical neurons (data not shown), and this is in agreement with other studies (Sobreviela et al, 1994;Holtzman et al, 1995). In addition, Domenici et al (1994b) found that the number of basal forebrain neurons labeled by a local injection of radiolabeled NGF into rat occipital cortex was one to two orders of magnitude greater than the number of labeled cortical neurons near the injection site, depending on the age of the animal.…”

Section: Possible Actions Of Ngf On Cortical Neuronssupporting

confidence: 92%

“…Almost all neurons expressing NGF receptors are also positive for the cholinergic marker ChAT (p75 NTR : Dawbarn et al, 1988;Batchelor et al, 1989;TrkA: Sobreviela et al, 1994). In the cerebral cortex, axons that express p75 NTR (Pioro and Cuello, 1990) and TrkA (Sobreviela et al, 1994;Hu et al, 1997) probably arise from the basal forebrain, and basal forebrain neurons retrogradely transport NGF exogenously applied to cortex (Seiler and Schwab, 1984;Domenici et al, 1994b). Since the cortical projections of cat basal forebrain neurons do not cross the midline (Ribak and Kramer, 1982;Irle and Markowitsch, 1984), it is possible to assay the e¡ects of delivering NGF locally to the axons of these neurons by comparing basal forebrain nuclei in a hemisphere in which NGF was infused to the corresponding nuclei in a control hemisphere.…”

Section: Local Cortical Ngf Infusion Increases the Number Of Basal Fomentioning

confidence: 99%

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Infusion of nerve growth factor (NGF) into kitten visual cortex increases immunoreactivity for NGF, NGF receptors, and choline acetyltransferase in basal forebrain without affecting ocular dominance plasticity or column development

Silver¹,

Fagiolini²,

Gillespie³

et al. 2001

Neuroscience

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AbstractöIntracerebroventricular or intracortical administration of nerve growth factor (NGF) has been shown to block or attenuate visual cortical plasticity in the rat. In cats and ferrets, the e¡ects of exogenous NGF on development and plasticity of visual cortex have been reported to be small or nonexistent. To determine whether locally delivered NGF a¡ects ocular dominance column formation or the plasticity produced by monocular deprivation in cats at the height of the critical period, we infused recombinant human NGF into the primary visual cortex of kittens using an implanted cannula minipump. NGF had no e¡ect on the normal developmental segregation of geniculocortical a¡erents into ocular dominance columns as determined both physiologically and anatomically. The plasticity of binocular visual cortical responses induced by monocular deprivation was also normal in regions of immunohistochemically detectable NGF infusion, as measured using intrinsic signal optical imaging and single-unit electrophysiology. Immunohistochemical analysis of the basal forebrain regions of the same animals demonstrated that the NGF infused into cortex was biologically active, producing an increase in the number of NGF-, TrkA-, p75 NTR -, and choline acetyltransferase-positive neurons in basal forebrain nuclei in the hemisphere ipsilateral to the NGF minipump compared to the contralateral basal forebrain neurons.We conclude that NGF delivered locally to axon terminals of cholinergic basal forebrain neurons resulted in increases in protein expression at the cell body through retrograde signaling. ß 2001 IBRO. Published by Elsevier Science Ltd. All rights reserved.Key words: TrkA, p75, choline acetyltransferase, retrograde transport, acetylcholine, monocular deprivation.Monocular deprivation (MD) is a well-studied model of experience-dependent cortical plasticity. When one eye of an animal is occluded during a critical period of development, that eye loses most of its ability to drive neurons in primary visual cortex, shifting the distribution of visual responses to favor the nondeprived eye (Wiesel and Hubel, 1963). Intracerebroventricular (ICV) administration of the neurotrophin nerve growth factor (NGF) to rats during a critical period of development blocks the e¡ects of MD (Domenici et al., 1991;Ma¡ei et al., 1992). This has been demonstrated at several levels of analysis, including physiological, anatomical, immunocytochemical, and behavioral (for a review, see Cellerino and Ma¡ei, 1996). Infusion of NGF directly into developing rat visual cortex also prevents physiological ocular dominance plasticity (Lodovichi et al., 2000).In addition, anti-NGF antibodies prevent normal anatomical and physiological development of the visual system when they are administered to rats by implantation of antibody-secreting hybridoma cells in the lateral ventricle (Berardi et al., 1994). These antibodies also prolong the critical period for ocular dominance plasticity in the rat (Domenici et al., 1994a). Together, these results demonstrate an imp...

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Section: Possible Actions Of Ngf On Cortical Neuronssupporting

confidence: 92%

Section: Local Cortical Ngf Infusion Increases the Number Of Basal Fomentioning

confidence: 99%

Infusion of nerve growth factor (NGF) into kitten visual cortex increases immunoreactivity for NGF, NGF receptors, and choline acetyltransferase in basal forebrain without affecting ocular dominance plasticity or column development

Silver¹,

Fagiolini²,

Gillespie³

et al. 2001

Neuroscience

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“…To examine whether Lhx7 is required in the mammalian forebrain to maintain the unique identity of CSIs, we compared the expression of the cholinergic markers ChAT, vesicular acetylcholine transporter (VAChT), and TrkA (19,20) in the striatum of adult ChAT::Cre;Lhx7 +/LacZ (control) and ChAT::Cre;Lhx7 fl/LacZ (conditional mutant) mice. As expected, CSIs coexpressing ChAT, VAChT, and TrkA were readily detected in control animals ( Fig.…”

Section: Resultsmentioning

confidence: 99%

Transcription factor LIM homeobox 7 (Lhx7) maintains subtype identity of cholinergic interneurons in the mammalian striatum

Lopes

Wijk

Neves

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

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The generation and maintenance of a plethora of neuronal subtypes is essential for normal brain function. Nevertheless, little is known about the molecular mechanisms that maintain the defining characteristics of neurons following their initial postmitotic specification. Using conditional gene ablation in mice, we demonstrate here that the homeodomain protein LIM homeobox (Lhx)7 is essential for maintaining the morphological and molecular characteristics of cholinergic interneurons of the striatum. Lhx7-depleted cholinergic interneurons extinguish expression of several subtype-specific markers, including choline acetyl transferase and Isl1, and are respecified into Lhx6-expressing mature GABAergic interneurons. Additional expression studies support a model where Lhx7 controls the choice between cholinergic or GABAergic identity by gating a cross inhibitory regulation between Isl1 and Lhx6. By demonstrating that the switch between alternative striatal interneuron fates depends on persistent activity of a single transcription factor, we provide evidence that the intrinsic plasticity of mammalian forebrain neuronal subtypes is maintained after the initial specification and lineage commitment and possibly throughout life.neuronal subtype specification | medial ganglionic eminence-derived interneurons T he information-processing ability of the nervous system depends on the formation of functional neuronal circuits composed of a highly heterogeneous population of neurons. Recent progress has identified molecular cascades that control the generation of distinct neuronal subtypes during development (1-3) but the mechanisms that maintain the identity of neurons following lineage commitment and differentiation are currently unclear. Identifying such mechanisms is critical for understanding phenotypic plasticity in the nervous system and, ultimately, influencing its ability to adjust during normal development, disease, or injury.The striatum is a subcortical structure that integrates multiple inputs from the cortex, thalamus, and midbrain and relays information to the output domains of the basal ganglia via its principal population of projection neurons (4, 5). Balanced striatal output, which is critical for motor and cognitive activity, depends on local circuits controlled by distinct subpopulations of GABAergic and cholinergic interneurons (5). Both GABAergic and cholinergic striatal interneurons (GSIs and CSIs, respectively) are derived from Nkx2.1-expressing progenitors of the medial ganglionic eminence (MGE) (6, 7), which, upon exit from the cell cycle, express the LIM homeodomain (LIM HD) transcription factors LIM homeobox 6 (Lhx6) and LIM homeobox 7 (Lhx7) (8). A subset of these precursors maintains expression of Lhx6 and differentiate into mature GABAergic interneurons expressing the neuropeptide somatostatin (Sst) or the calcium-binding protein parvalbumin (Pv) (9, 10). The remaining precursors induce expression of the LIM HD protein Isl1, down-regulate Lhx6, and differentiate into CSIs (8). In adult animals, all ...

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“…The p75 receptor is distributed throughout the dentate in a pattern resembling the AChE-containing septodentate input (Peterson et al, 1994). In addition, the p75 and the TrkA receptors are distributed in the dentate similarly to the septal input (Sobreviela et al, 1994;Dougherty and Milner, 1999;Barker-Gibb et al, 2001). The colocalization of p75 and TrkA in the septal fibers may be an important property dictating the probability of high-affinity binding (Hempstead et al, 1991;Esposito et al, 2001) and the consequent proliferation of cholinergic synapses.…”

Section: Discussionmentioning

confidence: 99%

Adeno-Associated Virus Vector Expressing Nerve Growth Factor Enhances Cholinergic Axonal Sprouting after Cortical Injury in Rats

et al. 2003

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Nerve growth factor (NGF) is known to promote both the survival of cholinergic neurons after injury and the regeneration of damaged cholinergic axons. Recent evidence has implicated NGF in the regulation of cholinergic axonal sprouting by intact neurons projecting to the hippocampus of rats, sustaining a lesion of the entorhinal cortex. We explored the possibility that NGF may regulate this lesioninduced cholinergic sprouting by injecting recombinant adeno-associated virus (rAAV) vector expressing NGF and green fluorescent protein (GFP) into the dentate gyrus of rats that were subsequently given unilateral entorhinal lesions. Sprague Dawley rats were unilaterally injected with (1) rAAV vector expressing NGF and GFP or (2) rAAV vector expressing GFP. Fourteen days after injection, the animals received lesions of the entorhinal area ipsilateral to the virus injection. Four days after lesion, GFP expression and the septodentate sprouting response in the dentate gyrus were assessed. Optical densitometric analyses revealed a significant increase in acetylcholinesterase label (a marker for cholinergic septodentate sprouting) in the ipsilateral outer molecular layer of the dentate gyrus in rats injected with rAAV vector expressing NGF. Thus, NGF-expressing rAAV vector enhanced the sprouting response of intact cholinergic neurons after unilateral entorhinal lesions in rats.

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TrkA‐immunoreactive profiles in the central nervous system: Colocalization with neurons containing p75 nerve growth factor receptor, choline acetyltransferase, and serotonin

Cited by 304 publications

References 96 publications

Infusion of nerve growth factor (NGF) into kitten visual cortex increases immunoreactivity for NGF, NGF receptors, and choline acetyltransferase in basal forebrain without affecting ocular dominance plasticity or column development

Infusion of nerve growth factor (NGF) into kitten visual cortex increases immunoreactivity for NGF, NGF receptors, and choline acetyltransferase in basal forebrain without affecting ocular dominance plasticity or column development

Transcription factor LIM homeobox 7 (Lhx7) maintains subtype identity of cholinergic interneurons in the mammalian striatum

Adeno-Associated Virus Vector Expressing Nerve Growth Factor Enhances Cholinergic Axonal Sprouting after Cortical Injury in Rats

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