Tritrichomonas foetus: induced division synchrony by hydroxyurea

Ribeiro, Karla Consort; Arnholdt, Andrea Cristina Vetö; Benchimol, Marlene

doi:10.1007/s00436-002-0628-1

Cited by 15 publications

(8 citation statements)

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“…3c). Previously published data reported the occurrence of such vacuolization processes in other microorganisms (Christensen et al, 1998;Sperandio et al, 2000;Wyllie & Goldstein, 2001), including T. foetus (Benchimol, 1999(Benchimol, , 2001Ribeiro et al, 2002;Mariante et al, 2003;Madeiro-da-Costa & Benchimol, 2004), when they are under stress conditions such as drug treatments. Cytoplasmic vacuolization is often associated with an autophagic process which in turn, is associated with cell death (Mariante et al, 2006).…”

Section: Discussionmentioning

confidence: 96%

“…Cytoplasmic vacuolization is often associated with an autophagic process which in turn, is associated with cell death (Mariante et al, 2006). Previously published data reported the occurrence of such vacuolization processes in other microorganisms (Christensen et al, 1998;Sperandio et al, 2000;Wyllie & Goldstein, 2001), including T. foetus (Benchimol, 1999(Benchimol, , 2001Ribeiro et al, 2002;Mariante et al, 2003;Madeiro-da-Costa & Benchimol, 2004), when they are under stress conditions such as drug treatments.…”

Section: Discussionmentioning

confidence: 98%

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Effects of three benzimidazoles on growth, general morphology and ultrastructure ofTritrichomonas foetus

Carvalho

Gadelha

2007

FEMS Microbiology Letters

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Tritrichomonas foetus is a venereal pathogen of cattle, which causes infertility, early embryonic death or abortion. In order to evaluate the potential trichomonicidal activity of benzimidazoles, the effects of thiabendazole, mebendazole and albendazole were analyzed on the multiplication, general morphology and ultrastructure of T. foetus. It was found that mebendazole presented the highest IC(50%) (2.3 microM), when compared with albendazole (IC(50%)=9.4 microM) and thiabendazole (IC(50%)=142.6 microM), and that such effects were irreversible. Concerning microscopic analysis, thiabendazole- and mebendazole-treated cells presented increased volume, internalization of the flagella, disruption or multiplication of the nucleus, multiple organelles and cytoplasmic vacuolization. Albendazole-treated cells exhibited slight alterations, because the parasite became slightly rounded, its flagella were not internalized but the cytoplasm was vacuolated. Mebendazole was indeed highly effective as an in vitro trichomonicidal agent, and this might open up new possibilities for the use of mebendazole in the therapy of bovine trichomoniasis.

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 98%

Effects of three benzimidazoles on growth, general morphology and ultrastructure ofTritrichomonas foetus

Carvalho

Gadelha

2007

FEMS Microbiology Letters

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“…Large vacuoles were also reported in G. intestinalis submitted to oxidative stress (Lloyd et al 2000) in L. donovani after induction with antimicrobial peptides (Bera et al 2003), and in T. foetus treated with other drugs, such as hydroxyurea, fibronectin, hydrogen peroxide, nocodazole, or colchicine (Benchimol 1999(Benchimol , 2001Ribeiro et al 2002;Mariante et al 2003;Madeiro da Costa and Benchimol 2004).…”

Section: Discussionmentioning

confidence: 84%

Cell death in trichomonads: new insights

Mariante

Vancini

Benchimol

2005

Histochem Cell Biol

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Tritrichomonas foetus is an amitochondriate parasite that possesses hydrogenosomes, unusual anerobic energy-producing organelles. In these organisms the "mitochondrial cell death machinery" is supposed to be absent, and the mechanisms that lead to cell demise remain to be elucidated. The presence of a cell death program in trichomonads has already been reported, suggesting the existence of a caspase-like execution pathway in such organisms. Here we demonstrate the alterations provoked by the fungicide griseofulvin and raise the possibility that other cell death pathways may exist in T. foetus. Dramatic changes in trichomonads morphology are presented after griseofulvin treatment, such as intense plasma membrane and nuclear envelope blebbing, nucleus fragmentation, and an abnormal number of oversized vacuoles. One important finding was the exposition of phosphatidylserine (PS) in the outer leaflet of the plasma membrane in cells after drug treatment, and also the presence of a high amount of misshapen flagella and tubulin precipitates as vacuolar contents, suggesting an autophagic process of abnormal cellular elements. Interestingly, immunoreactivity for activated caspase-3 was not detected during griseofulvin treatment, a finding distinct from the observed when this cell was treated with H(2)O(2). The possibility of the existence of different pathways to cell death in trichomonads is discussed.

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“…Although several studies using drugs have been successfully performed in different cell types, only very few studies have focused on using drug treatments in important parasites such as T. foetus (Silva-Filho and De Souza 1986;Benchimol 1996;Kulda 1999;Ribeiro et al 2002), although some reports on work done in Tritrichomonas vaginalis are available (Capuccinelli and Varesio 1975;Juliano et al 1986a;Noe¨l et al 2003).…”

Section: Introductionmentioning

confidence: 96%

The effect of drugs on cell structure of Tritrichomonas foetus

Costa

Benchimol

2004

Parasitology Research

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The effects of the microtubule affecting drugs taxol, nocodazole and colchicine on the cell cycle and ultrastructure of Tritrichomonas foetus, a protist parasite of cattle, were studied. Alterations in the cytoskeleton, motility and organellar ultrastructure were followed using anti-tubulin antibodies and fluorescence microscopy, scanning- and transmission-electron microscopy. Flow cytometry was also used to analyze the effect of the drugs on the cell cycle. T. foetus was treated with 10 microM taxol, 15 microM nocodazole or 1.5 mM colchicine for 12 h. The first effect observed was pseudocyst formation and alterations in cell motility. The cell cycle was affected and the cells have blocked cytokinesis, but not karyokinesis. The behavior of Golgi, hydrogenosomes and vacuoles was analyzed. The following effects were seen following drug treatments: (1) cell motility was altered and flagella internalized; (2) microtubules of the pelta-axostyle complex were not depolymerized and the axostyle assumed a curved form; (3) hydrogenosomes were of abnormal size and shape; (4) cells became multinucleate; (5) the division process was blocked in cytokinesis; (6) autophagic vacuoles containing a large amount of microtubules were seen; (7) axoneme organization was altered; (8) zoids were formed; (9) signs of cell death, such as membrane blebbing, were observed.

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Tritrichomonas foetus: induced division synchrony by hydroxyurea

Cited by 15 publications

References 9 publications

Effects of three benzimidazoles on growth, general morphology and ultrastructure ofTritrichomonas foetus

Effects of three benzimidazoles on growth, general morphology and ultrastructure ofTritrichomonas foetus

Cell death in trichomonads: new insights

The effect of drugs on cell structure of Tritrichomonas foetus

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