Triton X-114 phase partitioning in plant protein purification

PARTIAL PURIFICATION AND CHARACTERIZATION OF A CALCIUM‐DEPENDENT ALKALINE PHOSPHATASE FROM THE CYANOBACTERIUM ARTHROSPIRA PLATENSIS 1

“…Although TX‐114 and TX‐100 have a similar capacity to digest membranes, a solution of TX‐114 is turbid at room temperature and separates into two phases at ∼35°C because of the formation of large micellar aggregates (Sánchez‐Ferrer et al. 1994).…”

“…1994). This characteristic is useful for removing hydrophobic proteins and compounds such as phenols, chls, and lipids (Sánchez‐Ferrer et al. 1994, Pérez‐Gilabert et al.…”

Quantitative Determination of Zwitterionic Detergents Using Salt-Induced Phase Separation of Triton X-100

“…This phase separation could be induced only when salt was added (salt-induced phase separation). A comparable behavior of nonionic detergents has previously been described for members of the Triton, Brij, and Tween series (3)(4)(5). As far as we know, such a delay of phase separation for nonionic detergents, caused by the presence of zwitterionic detergents, is a new phenomenon not described until now, which is of common interest for scientists working with membrane proteins and detergents.…”

High‐Performance Liquid Chromatography of Biological Macromolecules

“…Detergents may be needed to get and keep solutes in solution, such as use of the mild nondenaturing detergent Triton X-114 as a phase-partitioning agent in the purification of plant proteins. 697 Micelles may also be valuable, as in the extraction of hydrophobic peptides. 698 Partitioning in aqueous two-phase systems is a batchoperated soft-separation process for cells, organelles, and membranes, 699,700 that can be used as a sample preparation step for high-performance separation.…”