Tritium labelling of several cancer pathway agents at high specific activity

Tritium NMR: A compilation of data and a practical guide

“…Ahern and coauthors [ 62 ] performed tritium labeling of several cancer pathway agents at high specific activity and recorded their tritium NMR spectra. The resonances at 8.56, 8.28, and 8.13 ppm were attributed to accordingly, sixth, first, and third carbons, which was confirmed by several proton coupled tritium NMR experiments; see Figure 41.…”

“…NMR spectra of [1,3,6‐ 3 H] benzo[ a ]pyrene recorded in CDCl 3 at 320 MHz: (a) proton decoupled tritium NMR; (b) proton coupled tritium NMR; and (c) proton coupled proton NMR. Reproduced with minor editing privilege from Ahern et al [ 62 ] with the permission of Springer Nature…”

“…Ahern and coauthors [62] performed tritium labeling of several cancer pathway agents at high specific activity and recorded their tritium NMR spectra. The resonances at 8.56, 8.28, and 8.13 ppm were attributed to F I G U R E 3 0 Tritium NMR spectrum of dopamine receptor stimulant N-0923, recorded in CDCl 3 at 320 MHz.…”

“…Besides the expected aromatic resonance at δ 7.35 ppm, the tritium NMR spectrum of pargyline clearly revealed that a significant amount of tritium had also been incorporated to the benzyl position, which is seen in the tritium spectrum as two peaks centered at δ 3.57 ppm. The larger singlet at δ 3.59 ppm was assigned by the authors to the isotopomer of pargyline with a single benzyl tritium label, whereas the upfield singlet at δ 3.55 ppm was ascribed to the [62] with the permission of Springer Nature doubly labeled benzyl tritium species. On the other hand, a proton decoupled tritium NMR spectrum of caracemide displayed the expected single methyl resonance at δ 2.82 ppm confirming exclusive monolabeling in this case.…”

Radiochemistry

“…A tritritiomethyl Grignard reagent was used 44 to introduce tritium into a methylpentane cannabidiol. Other compounds recently labelled with tritium have included benzo[a]pyrene, 45 tryptamine and etodolac, 46 primulene, 47 thymidine, 48 the peptide PACAP-38 49 and a sigma-1 receptor, (+)-pentazocine. 50 Carbon ( 11 C & 14 C) Whilst it is interesting to report 51 the direct use of cyclotron produced [ 11 C] carbon dioxide as a tracer in fruit development, it is more usual for [ 11 C] methyl iodide to be prepared 52 which can then be used to introduce labelled methyl groups into a variety of compounds.…”