Tritiation of two potent catecholamine beta-adrenergic receptor agonists

Tritium NMR: A compilation of data and a practical guide

“…Ahern and coworkers [ 71 ] performed tritiation of the potent catecholamine beta‐adrenergic receptor agonist for the beta‐adrenergic receptor, isoproterenol. As a result of the performed tritiation, either one or two tritiums were incorporated into the basic structure, as shown in Figure 48.…”

“…Tritium proton decoupled NMR spectrum of [7‐ 3 H] ( RS )‐isoproterenol (top) and [7,8‐ 3 H] ( RS )‐isoproterenol (bottom) recorded in D 2 O at 320 MHz. Reproduced with minor editing privilege from Ahern et al [ 71 ] with the permission of Springer Nature…”

“…Reproduced with minor editing privilege from Rauh et al [60] with the permission of John Wiley and Sons F I G U R E 4 0 Tritium proton decoupled NMR spectrum of the tritium labeled chlorpromazine (top) and cytochalasin B (bottom) recorded in CD 3 OD at 320 MHz. Reproduced with minor editing privilege from Ahern et al [61] with the permission of Springer Nature Ahern and coworkers [71] performed tritiation of the potent catecholamine beta-adrenergic receptor agonist for the beta-adrenergic receptor, isoproterenol. As a result of the performed tritiation, either one or two tritiums were incorporated into the basic structure, as shown in Figure 48.…”

“…Illustrated in Figures 53 and 54 are accordingly, the 1D hetero-TOCSY 1 H and 3 H NMR spectra with the [7, H] (RS)-isoproterenol (bottom) recorded in D 2 O at 320 MHz. Reproduced with minor editing privilege from Ahern et al [71] with the permission of Springer Nature complete suppression of the uncoupled resonances of the 3 H-labeled trifluoromethyldiazirine substituted paclitaxel derivative and the 1 H and 3 H NMR spectra of the 3 Hlabeled derivative of the same compound with and without heteronuclear decoupling. Shown in Figure 55 are the expanded contour plots of the 2D 1 H-3 H hetero-TOCSY spectra of the paclitaxel derivative using different modifications of this basic pulse sequence (the details are provided in figure caption).…”